Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Jan;25(1):162–164. doi: 10.1128/jcm.25.1.162-164.1987

Comparison of Sabouraud dextrose and Pagano-Levin agar media for detection and isolation of yeasts from oral samples.

L P Samaranayake, T W MacFarlane, M I Williamson
PMCID: PMC265849  PMID: 3539988

Abstract

The sensitivities of Sabouraud dextrose agar and modified Pagano-Levin agar for the primary isolation of yeasts and the recovery of multiple yeast species from single clinical samples were compared by using oral-rinse samples. Although there was a highly significant positive correlation between the numbers of yeasts recovered from both media, modified Pagano-Levin agar was far superior in detecting multiple yeast species in a single sample. Of 150 oral samples containing yeasts, 23 (15.3%) contained more than one yeast species. The most frequent combination of different yeasts was Candida albicans and Torulopsis glabrata.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison R. T. An evaluation of Pagano-Levin medium in a quantitative study of Candida albicans: preliminary communication. J Med Lab Technol. 1967 Jul;24(3):199–202. [PubMed] [Google Scholar]
  2. Eschenbach D. A., Buchanan T. M., Pollock H. M., Forsyth P. S., Alexander E. R., Lin J. S., Wang S. P., Wentworth B. B., MacCormack W. M., Holmes K. K. Polymicrobial etiology of acute pelvic inflammatory disease. N Engl J Med. 1975 Jul 24;293(4):166–171. doi: 10.1056/NEJM197507242930403. [DOI] [PubMed] [Google Scholar]
  3. Gilchrist J. E., Donnelly C. B., Peeler J. T., Campbell J. E. Collaborative study comparing the spiral plate and aerobic plate count methods. J Assoc Off Anal Chem. 1977 Jul;60(4):807–812. [PubMed] [Google Scholar]
  4. KUTSCHER A. H., SEGUIN L., ZEGARELLI E. V., RANKOW R. M., CAMPBELL J. B., MERCADANTE J. Pagano-Levin culture medium for differentiation of Candida albicans. American type culture collection studies. I. Antibiot Chemother (Northfield) 1959 Nov;9:649–659. [PubMed] [Google Scholar]
  5. Kiani D., Quinn E. L., Burch K. H., Madhavan T., Saravolatz L. D., Neblett T. R. The increasing importance of polymicrobial bacteremia. JAMA. 1979 Sep 7;242(10):1044–1047. [PubMed] [Google Scholar]
  6. PAGANO J., LEVIN J. D., TREJO W. Diagnostic medium for differentiation of species of Candida. Antibiot Annu. 1957;5:137–143. [PubMed] [Google Scholar]
  7. Stedham M. A., Kelley D. C., Coles E. H. Modified Pagano Levin medium to isolate Candida species. Appl Microbiol. 1966 Jul;14(4):525–528. doi: 10.1128/am.14.4.525-528.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Walsh T. J., Venanzi W. E., Dixon D. M. Quantification of medically important Candida species and Torulopsis glabrata by a spiral inoculation system: correlation with pour plate and spread plate methods. J Clin Microbiol. 1985 Nov;22(5):745–747. doi: 10.1128/jcm.22.5.745-747.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Yamane N., Saitoh Y. Isolation and detection of multiple yeasts from a single clinical sample by use of Pagano-Levin agar medium. J Clin Microbiol. 1985 Feb;21(2):276–277. doi: 10.1128/jcm.21.2.276-277.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES