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. 1987 Jan;25(1):191–192. doi: 10.1128/jcm.25.1.191-192.1987

Modified indirect immunofluorescence test for serotyping large numbers of Ureaplasma urealyticum clinical isolates.

A Naessens, S Lauwers
PMCID: PMC265859  PMID: 3539992

Abstract

A technical modification of the indirect immunofluorescence test for serotyping Ureaplasma urealyticum clinical isolates is described. The use of a tissue culture plate filled with ureaplasma agar made the serotyping easier to perform and proved to be very handy, especially for performance of large series.

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Selected References

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  1. Black F. T., Krogsgaard-Jensen A. Application of indirect immunofluorescence, indirect haemagglutination and polyacrylamide-gel electrophoresis to human T-mycoplasmas. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):345–353. doi: 10.1111/j.1699-0463.1974.tb02336.x. [DOI] [PubMed] [Google Scholar]
  2. Black F. T. Modifications of the growth inhibition test and its application to human T-mycoplasmas. Appl Microbiol. 1973 Apr;25(4):528–533. doi: 10.1128/am.25.4.528-533.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cassell G. H., Davis R. O., Waites K. B., Brown M. B., Marriott P. A., Stagno S., Davis J. K. Isolation of Mycoplasma hominis and Ureaplasma urealyticum from amniotic fluid at 16-20 weeks of gestation: potential effect on outcome of pregnancy. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):294–302. [PubMed] [Google Scholar]
  4. Cassell G. H., Younger J. B., Brown M. B., Blackwell R. E., Davis J. K., Marriott P., Stagno S. Microbiologic study of infertile women at the time of diagnostic laparoscopy. Association of Ureaplasma urealyticum with a defined subpopulation. N Engl J Med. 1983 Mar 3;308(9):502–505. doi: 10.1056/NEJM198303033080906. [DOI] [PubMed] [Google Scholar]
  5. Crăcea E., Constantinescu S., Lazar M. Serotypes of Ureaplasma urealyticum isolated from patients with nongonococcal urethritis and gonorrhea and from asymptomatic urethral carriers. Sex Transm Dis. 1985 Oct-Dec;12(4):219–223. doi: 10.1097/00007435-198510000-00010. [DOI] [PubMed] [Google Scholar]
  6. Embree J. E., Krause V. W., Embil J. A., MacDonald S. Placental infection with Mycoplasma homonis and Ureaplasma urealyticum: clinical correlation. Obstet Gynecol. 1980 Oct;56(4):475–481. [PubMed] [Google Scholar]
  7. Foulon W., Naessens A., Dewaele M., Lauwers S., Amy J. J. Chronic Ureaplasma urealyticum amnionitis associated with abruptio placentae. Obstet Gynecol. 1986 Aug;68(2):280–282. [PubMed] [Google Scholar]
  8. Hewish M. J., Birch D. F., Fairley K. F. Ureaplasma urealyticum serotypes in urinary tract disease. J Clin Microbiol. 1986 Jan;23(1):149–154. doi: 10.1128/jcm.23.1.149-154.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lin J. S., Kass E. H. Fourteen serotypes of ureaplasma urealyticum (T-mycoplasmas) demonstrated by the complement-dependent mycoplasmacidal test. Infection. 1980;8(4):152–155. doi: 10.1007/BF01639122. [DOI] [PubMed] [Google Scholar]
  10. Lin J. S., Kendrick M. I., Kass E. H. Serologic typing of human genital T-mycoplasmas by a complement-dependent mycoplasmacidal test. J Infect Dis. 1972 Dec;126(6):658–663. doi: 10.1093/infdis/126.6.658. [DOI] [PubMed] [Google Scholar]
  11. Naessens A., Foulon W., Volckaert M., Amy J. J., Lauwers S. Cervical and placental colonization by Ureaplasma urealyticum in pregnant women and fetal outcome. J Infect Dis. 1983 Aug;148(2):333–333. doi: 10.1093/infdis/148.2.333. [DOI] [PubMed] [Google Scholar]
  12. Panangala V. S., Lein D. H. Development of a template for use in immunofluorescent identification of mycoplasmas. J Clin Microbiol. 1978 Apr;7(4):399–400. doi: 10.1128/jcm.7.4.399-400.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Piot P. Distribution of eight serotypes of Ureaplasma urealyticum in cases of non-gonococcal urethritis and of gonorrhoea, and in healthy persons. Br J Vener Dis. 1976 Aug;52(4):266–268. doi: 10.1136/sti.52.4.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Purcell R. H., Taylor-Robinson D., Wong D., Chanock R. M. Color test for the measurement of antibody to T-strain mycoplasmas. J Bacteriol. 1966 Jul;92(1):6–12. doi: 10.1128/jb.92.1.6-12.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Robertson J. A., Stemke G. W. Expanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans. J Clin Microbiol. 1982 May;15(5):873–878. doi: 10.1128/jcm.15.5.873-878.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rosendal S., Black F. T. Direct and indirect immunofluorescence of unfixed and fixed Mycoplasma colonies. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(4):615–622. doi: 10.1111/j.1699-0463.1972.tb00186.x. [DOI] [PubMed] [Google Scholar]
  17. Shepard M. C., Lunceford C. D. Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976 Jun;3(6):613–625. doi: 10.1128/jcm.3.6.613-625.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shepard M. C., Lunceford C. D. Serological typing of Ureaplasma urealyticum isolates from urethritis patients by an agar growth inhibition method. J Clin Microbiol. 1978 Nov;8(5):566–574. doi: 10.1128/jcm.8.5.566-574.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stemke G. W., Robertson J. A. Modified colony indirect epifluorescence test for serotyping Ureaplasma urealyticum and an adaptation to detect common antigenic specificity. J Clin Microbiol. 1981 Nov;14(5):582–584. doi: 10.1128/jcm.14.5.582-584.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor-Robinson D. The role of mycoplasmas in non-gonococcal urethritis: a review. Yale J Biol Med. 1983 Sep-Dec;56(5-6):537–543. [PMC free article] [PubMed] [Google Scholar]

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