Abstract
Commercially available latex agglutination and coagglutination reagents were evaluated for their ability to detect bacterial antigens in the sera of 165 patients to determine their suitability for rapid diagnosis of pneumonia. These reagents were used to detect the polysaccharide capsular antigens of Haemophilus influenzae type b and Streptococcus pneumoniae in nonbacteremic patients known to be respiratory culture positive for these organisms. The reagents were unable to detect the polysaccharide antigens in sera from nonbacteremic patients. Patients with a clinical diagnosis of pneumonia who had respiratory or extrarespiratory infections with a variety of organisms were also tested. No evidence of cross-reactivity or of false-positive reactions was observed with either reagent. Because a negative agglutination test may occur during the course of a nonbacteremic infection, these reagents should not be used alone, and if used, they should be used only in conjunction with standard bacteriological tests.
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Selected References
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- Ingram D. L., Pearson A. W., Occhiuti A. R. Detection of bacterial antigens in body fluids with the Wellcogen Haemophilus influenzae b, Streptococcus pneumoniae, and Neisseria meningitidis (ACYW135) latex agglutination tests. J Clin Microbiol. 1983 Nov;18(5):1119–1121. doi: 10.1128/jcm.18.5.1119-1121.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Reilly R. J., Anderson P., Ingram D. L., Peter G., Smith D. H. Circulating polyribophosphate in Hemophilus influenzae, type b meningitis. Correlation with clinical course and antibody response. J Clin Invest. 1975 Oct;56(4):1012–1022. doi: 10.1172/JCI108148. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scheifele D. W., Ward J. I., Siber G. R. Advantage of latex agglutination over countercurrent immunoelectrophoresis in the detection of Haemophilus influenzae type b antigen in serum. Pediatrics. 1981 Dec;68(6):888–891. [PubMed] [Google Scholar]
- Shaw E. D., Darker R. J., Feldman W. E., Gray B. M., Pifer L. L., Scott G. B. Clinical studies of a new latex particle agglutination test for detection of Haemophilus influenzae type b polyribose phosphate antigen in serum, cerebrospinal fluid, and urine. J Clin Microbiol. 1982 Jun;15(6):1153–1156. doi: 10.1128/jcm.15.6.1153-1156.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Suksanong M., Dajani A. S. Detection of Haemophilus influenzae type b antigens in body fluids, using specific antibody-coated staphylococci. J Clin Microbiol. 1977 Jan;5(1):81–85. doi: 10.1128/jcm.5.1.81-85.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thirumoorthi M. C., Dajani A. S. Comparison of staphylococcal coagglutination, latex agglutination, and counterimmunoelectrophoresis for bacterial antigen detection. J Clin Microbiol. 1979 Jan;9(1):28–32. doi: 10.1128/jcm.9.1.28-32.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward J. I., Siber G. R., Scheifele D. W., Smith D. H. Rapid diagnosis of Hemophilus influenzae type b infections by latex particle agglutination and counterimmunoelectrophoresis. J Pediatr. 1978 Jul;93(1):37–42. doi: 10.1016/s0022-3476(78)80596-4. [DOI] [PubMed] [Google Scholar]
- Welch D. F., Hensel D. Evaluation of Bactogen and Phadebact for detection of Haemophilus influenzae type b antigen in cerebrospinal fluid. J Clin Microbiol. 1982 Nov;16(5):905–908. doi: 10.1128/jcm.16.5.905-908.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]