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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Feb;25(2):427–429. doi: 10.1128/jcm.25.2.427-429.1987

ATP measurements obtained by luminometry provide rapid estimation of Ureaplasma urealyticum growth.

M E Stemler, G W Stemke, J A Robertson
PMCID: PMC265915  PMID: 3818931

Abstract

ATP content obtained by luciferin-luciferase luminometry with commercially available reagents provided rapid estimates of Ureaplasma urealyticum populations. Each cell contained about 4.7 X 10(-18) mol of ATP. We could detect 10(4) CCU50 (color change unit50) per 100 microliters. We correlated urease activity with growth and confirmed the differential response of ureaplasma strains to Mn2+.

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Selected References

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  1. Beaman K. D., Pollack J. D. Adenylate energy charge in Acholeplasma laidlawii. J Bacteriol. 1981 Jun;146(3):1055–1058. doi: 10.1128/jb.146.3.1055-1058.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beaman K. D., Pollack J. D. Synthesis of adenylate nucleotides by Mollicutes (mycoplasmas). J Gen Microbiol. 1983 Oct;129(10):3103–3110. doi: 10.1099/00221287-129-10-3103. [DOI] [PubMed] [Google Scholar]
  3. Beckers B., Lang H. R. Rapid diagnosis of bacteraemia by measuring bacterial adenosine triphosphate in blood culture bottles using bioluminescence. Med Microbiol Immunol. 1983;172(2):117–122. doi: 10.1007/BF02124512. [DOI] [PubMed] [Google Scholar]
  4. Conn R. B., Charache P., Chappelle E. W. Limits of applicability of the firefly luminescence ATP assay for the detection of bacteria in clinical specimens. Am J Clin Pathol. 1975 Apr;63(4):493–501. doi: 10.1093/ajcp/63.4.493. [DOI] [PubMed] [Google Scholar]
  5. Fauchère J. L., Simonet M., Descamps P., Véron M. Détermination rapide de la concentration minimale inhibitrice d'un antibiotique par dosage de l'ATP bactérien. Ann Microbiol (Paris) 1982 Mar-Apr;133(2):293–299. [PubMed] [Google Scholar]
  6. Kimmich G. A., Randles J., Brand J. S. Assay of picomole amounts of ATP, ADP, and AMP using the luciferase enzyme system. Anal Biochem. 1975 Nov;69(1):187–206. doi: 10.1016/0003-2697(75)90580-1. [DOI] [PubMed] [Google Scholar]
  7. Lundin A., Richardsson A., Thore A. Continous monitoring of ATP-converting reactions by purified firefly luciferase. Anal Biochem. 1976 Oct;75(2):611–620. doi: 10.1016/0003-2697(76)90116-0. [DOI] [PubMed] [Google Scholar]
  8. Mackett D., Kessock-Philip S., Bascomb S., Easmon C. S. Evaluation of the Lumac kit for the detection of bacteriuria by bioluminescence. J Clin Pathol. 1982 Jan;35(1):107–110. doi: 10.1136/jcp.35.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McElroy W. D. The Energy Source for Bioluminescence in an Isolated System. Proc Natl Acad Sci U S A. 1947 Nov;33(11):342–345. doi: 10.1073/pnas.33.11.342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McWalter P. W. Determination of susceptibility of Staphylococcus aureus to methicillin by luciferin-luciferase assay of bacterial adenosine triphosphate. J Appl Bacteriol. 1984 Feb;56(1):145–150. doi: 10.1111/j.1365-2672.1984.tb04706.x. [DOI] [PubMed] [Google Scholar]
  11. McWalter P. W., Sharp C. A. Evaluation of a commercially available semi-automated bioluminescence system for bacteriuria screening. Eur J Clin Microbiol. 1982 Aug;1(4):223–227. doi: 10.1007/BF02019712. [DOI] [PubMed] [Google Scholar]
  12. Molin O., Nilsson L., Anséhn S. Rapid detection of bacterial growth in blood cultures by bioluminescent assay of bacterial ATP. J Clin Microbiol. 1983 Sep;18(3):521–525. doi: 10.1128/jcm.18.3.521-525.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Razin S. The mycoplasmas. Microbiol Rev. 1978 Jun;42(2):414–470. doi: 10.1128/mr.42.2.414-470.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Robertson J. A., Alfa M., Boatman E. S. Morphology of the cells and colonies of Mycoplasma hominis. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):232–239. [PubMed] [Google Scholar]
  15. Robertson J. A. Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma). J Clin Microbiol. 1978 Feb;7(2):127–132. doi: 10.1128/jcm.7.2.127-132.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Robertson J. A., Chen M. H. Effects of manganese on the growth and morphology of Ureaplasma urealyticum. J Clin Microbiol. 1984 Jun;19(6):857–864. doi: 10.1128/jcm.19.6.857-864.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robertson J. A., Stemke G. W. Expanded serotyping scheme for Ureaplasma urealyticum strains isolated from humans. J Clin Microbiol. 1982 May;15(5):873–878. doi: 10.1128/jcm.15.5.873-878.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. STREHLER B. L., TOTTER J. R. Firefly luminescence in the study of energy transfer mechanisms. I. Substrate and enzyme determination. Arch Biochem Biophys. 1952 Sep;40(1):28–41. doi: 10.1016/0003-9861(52)90070-2. [DOI] [PubMed] [Google Scholar]
  19. Stemke G. W., Robertson J. A. Comparison of two methods for enumeration of mycoplasmas. J Clin Microbiol. 1982 Nov;16(5):959–961. doi: 10.1128/jcm.16.5.959-961.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wong B. L., Shobe C. R. Single-step purification of urease by affinity chromatography. Can J Microbiol. 1974 Apr;20(4):623–630. doi: 10.1139/m74-095. [DOI] [PubMed] [Google Scholar]

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