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. 2008 Dec 18;150(4):1588–1596. doi: 10.1210/en.2008-1001

Figure 1.

Figure 1

Prolonged suppression of p160 coactivator expression by siRNA transfection and effect on MCF-7 cell growth. MCF-7 cells were transfected with 10 pmol total siRNA Silencer-negative control (siControl; C), SRC-1, SRC-2,or SRC-3 and then treated with VEH (0.1% ethanol), 1 nm E2, or 100 nm 4HT for 5 d. Subsequently expression of SRC-1, SRC-2, SRC-3, and actin was assessed by Western blotting (A) and cell number was determined by Coulter counter (B). Representative blots are shown and demonstrate that coactivator depletion is apparent 5 d after siRNA transfection and hormone treatment. The cell number data are normalized to values obtained for control siRNA-transfected and E2-treated cultures and represent the mean ± sem of three to four experiments. Statistical analyses were by ANOVA. *, P < 0.05 vs. similarly treated (VEH, E2, or 4HT), control siRNA-transfected cells.