Figure 2.

Effect of p160 coactivator depletion on cell cycle distribution in MCF-7 cells. MCF-7 cells were transfected with either control siRNA or siRNA against SRC1, SRC-2, or SRC-3 and subsequently treated with VEH (0.1% ethanol) or 10 nm E2 for 24 h. Thereafter cells were harvested, stained with propidium iodide, and subjected to flow-cytometric analysis. The data represent the mean ± sem of three to four experiments. Comparisons between VEH and E2-treated samples within the same siRNA treatment group were made by student t test (***, P < 0.001; **, P < 0.01; *, P < 0.05). Comparisons of the effect of siRNA treatments on VEH or E2 groups were done by one-way ANOVA (#, P < 0.05 vs. the respective siControl group).