Figure 8.

Sections showing hybrid glomeruli. Top panels are from separate samples processed for LacZ, lower panels show corresponding serial sections immunolabeled for laminin. (A and C) Host tissue is intensely blue and can easily be distinguished from graft (dashed black line demarcates margin between host and graft tissue). Note ingress of a number of host-derived cells into graft, and the formation of hybrid glomeruli (arrows) containing host (blue) endothelial cells. (B and D) Immunofluorescence images of serial sections doubly labeled for laminin α1 (green) and α5 (red) chains. Laminin α5 protein is present in GBMs of hybrid glomeruli. (* marks same tubule in serial sections). Reproduced with permission (ref. ¹⁰⁰).