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. 1987 Mar;25(3):531–535. doi: 10.1128/jcm.25.3.531-535.1987

Production of Vi monoclonal antibodies and their application as diagnostic reagents.

R S Tsang, P Y Chau
PMCID: PMC265979  PMID: 3571457

Abstract

Serum antibodies to Vi antigen were detected in mice immunized with the purified antigen but not with Vi-bearing Salmonella typhi whole cells. Fusion of the spleen cells from one of the Vi antibody-producing mice with NSI myeloma cells produced four stable hybridomas that secreted antibodies to Vi. Monoclonal antibodies from these four clones were all of the immunoglobulin G class and, as determined by competition, appeared to have the same epitope specificity. Despite their immunoglobulin G nature, mouse ascitic fluids induced by one of the hybridomas strongly agglutinated the Vi-positive strains of S. typhi, S. dublin, and Citrobacter strain 5396/38. Thus, 10 clinical isolates of S. typhi but not 98 strains of other bacteria were reactive in slide agglutination tests with the monoclonal antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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