Abstract
In order to develop a DNA-based assay to identify all Mycobacterium kansasii clinical isolates, a specific DNA probe was isolated in plasmid p6123. A total of 145 M. kansasii clinical isolates were collected from several countries and were examined with three probes by DNA hybridization. Of the 145 isolates, 115 (79%) were positive with the previously described probe pMK1-9 (Z. H. Huang, B. C. Ross, and B. Dwyer, J. Clin. Microbiol. 29:2125-2129, 1991), 129 (88%) were positive with the commercial Accu-probe assay (Gen-Probe), and 145 (100%) were positive with the p6123 probe. Southern blot analysis of EcoRI-digested M. kansasii chromosomal DNA with p6123 revealed that all Accu-probe-positive M. kansasii strains exhibited a 3-kb fragment, whereas all Accu-probe-negative M. kansasii strains displayed DNA fragments of variable molecular sizes. These results indicate that, unlike the previously described probes for M. kansasii, the fragment cloned into p6123 identified all 145 biochemically typical strains tested and provides an ideal target for future DNA-based speciation assays.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bailey W. C., Brown M., Buechner H. A., Weill H., Ichinose H., Ziskind M. Silico-mycobacterial disease in sandblasters. Am Rev Respir Dis. 1974 Aug;110(2):115–125. doi: 10.1164/arrd.1974.110.2.115. [DOI] [PubMed] [Google Scholar]
- Huang Z. H., Ross B. C., Dwyer B. Identification of Mycobacterium kansasii by DNA hybridization. J Clin Microbiol. 1991 Oct;29(10):2125–2129. doi: 10.1128/jcm.29.10.2125-2129.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johanson W. G., Jr, Nicholson D. P. Pulmonary disease due to Mycobacterium Kansasii. An analysis of some factors affecting prognosis. Am Rev Respir Dis. 1969 Jan;99(1):73–85. doi: 10.1164/arrd.1969.99.1.73. [DOI] [PubMed] [Google Scholar]
- Marks J. "Opportunist" mycobacteria in England and Wales. Tubercle. 1969 Mar;50(Suppl):78–80. [PubMed] [Google Scholar]
- Ross B. C., Jackson K., Yang M., Sievers A., Dwyer B. Identification of a genetically distinct subspecies of Mycobacterium kansasii. J Clin Microbiol. 1992 Nov;30(11):2930–2933. doi: 10.1128/jcm.30.11.2930-2933.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selik R. M., Starcher E. T., Curran J. W. Opportunistic diseases reported in AIDS patients: frequencies, associations, and trends. AIDS. 1987 Sep;1(3):175–182. [PubMed] [Google Scholar]
- Sherer R., Sable R., Sonnenberg M., Cooper S., Spencer P., Schwimmer S., Kocka F., Muthuswamy P., Kallick C. Disseminated infection with Mycobacterium kansasii in the acquired immunodeficiency syndrome. Ann Intern Med. 1986 Nov;105(5):710–712. doi: 10.7326/0003-4819-105-5-710. [DOI] [PubMed] [Google Scholar]
- Wolinsky E. Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis. 1979 Jan;119(1):107–159. doi: 10.1164/arrd.1979.119.1.107. [DOI] [PubMed] [Google Scholar]
- Yang M., Ross B. C., Dwyer B. Identification of an insertion sequence-like element in a subspecies of Mycobacterium kansasii. J Clin Microbiol. 1993 Aug;31(8):2074–2079. doi: 10.1128/jcm.31.8.2074-2079.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]