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. 1993 Oct;31(10):2783–2785. doi: 10.1128/jcm.31.10.2783-2785.1993

Direct DNA probe assay for Neisseria gonorrhoeae in pharyngeal and rectal specimens.

J S Lewis 1, O Fakile 1, E Foss 1, G Legarza 1, A Leskys 1, K Lowe 1, D Powning 1
PMCID: PMC266014  PMID: 8253983

Abstract

The direct detection of gonococcal DNA in rectal and pharyngeal specimens was evaluated by using a DNA probe-based assay (Gen-Probe, Inc., San Diego, Calif.). Rectal (234) and pharyngeal (608) swab specimens were obtained from 249 men and 372 women attending sexually transmitted disease clinics in Las Vegas and Reno, Nevada. The prevalence of gonococcal infection by culture at the pharyngeal and rectal sites was 2.9% (16 of 548 specimens) in women and 2.7% (8 of 294 specimens) in men. No false-positive reactions were observed among the 234 rectal specimens tested. Two probe-positive, culture-negative specimens were detected among the 361 pharyngeal specimens obtained from women. Both of these samples were confirmed as Neisseria gonorrhoeae by a probe competition assay. The overall correlation of the DNA probe test with pharyngeal and rectal cultures was 99.4% (837 of 842 cultures), with a sensitivity of 87.5% (21 of 24 cultures) and specificity of 99.7% (816 of 818 cultures). The positive and negative predictive values of the DNA assay were 91.3 and 99.8%, respectively. The direct DNA probe assay provides an alternative to culture screening for rectal and/or pharyngeal gonococcal infections.

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Selected References

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  1. Bro-Jorgensen A., Jensen T. Gonococcal pharyngeal infections. Report of 110 cases. Br J Vener Dis. 1973 Dec;49(6):491–499. doi: 10.1136/sti.49.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dillon J. R., Carballo M., Pauzé M. Evaluation of eight methods for identification of pathogenic Neisseria species: Neisseria-Kwik, RIM-N, Gonobio-Test, Minitek, Gonochek II, GonoGen, Phadebact Monoclonal GC OMNI Test, and Syva MicroTrak Test. J Clin Microbiol. 1988 Mar;26(3):493–497. doi: 10.1128/jcm.26.3.493-497.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Granato P. A., Franz M. R. Use of the Gen-Probe PACE system for the detection of Neisseria gonorrhoeae in urogenital samples. Diagn Microbiol Infect Dis. 1990 May-Jun;13(3):217–221. doi: 10.1016/0732-8893(90)90062-z. [DOI] [PubMed] [Google Scholar]
  4. Janda W. M., Zigler K. L., Bradna J. J. API QuadFERM+ with rapid DNase for identification of Neisseria spp. and Branhamella catarrhalis. J Clin Microbiol. 1987 Feb;25(2):203–206. doi: 10.1128/jcm.25.2.203-206.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kinghorn G. R., Rashid S. Prevalence of rectal and pharyngeal infection in women with gonorrhoea in Sheffield. Br J Vener Dis. 1979 Dec;55(6):408–410. doi: 10.1136/sti.55.6.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klein E. J., Fisher L. S., Chow A. W., Guze L. B. Anorectal gonococcal infection. Ann Intern Med. 1977 Mar;86(3):340–346. doi: 10.7326/0003-4819-86-3-340. [DOI] [PubMed] [Google Scholar]
  7. Limberger R. J., Biega R., Evancoe A., McCarthy L., Slivienski L., Kirkwood M. Evaluation of culture and the Gen-Probe PACE 2 assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis in endocervical specimens transported to a state health laboratory. J Clin Microbiol. 1992 May;30(5):1162–1166. doi: 10.1128/jcm.30.5.1162-1166.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Panke E. S., Yang L. I., Leist P. A., Magevney P., Fry R. J., Lee R. F. Comparison of Gen-Probe DNA probe test and culture for the detection of Neisseria gonorrhoeae in endocervical specimens. J Clin Microbiol. 1991 May;29(5):883–888. doi: 10.1128/jcm.29.5.883-888.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schmale J. D., Martin J. E., Jr, Domescik G. Observations on the culture diagnosis of gonorrhea in women. JAMA. 1969 Oct 13;210(2):312–314. [PubMed] [Google Scholar]
  10. Thin R. N., Shaw E. J. Diagnosis of gonorrhoea in women. Br J Vener Dis. 1979 Feb;55(1):10–13. doi: 10.1136/sti.55.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vlaspolder F., Mutsaers J. A., Blog F., Notowicz A. Value of a DNA probe assay (Gen-Probe) compared with that of culture for diagnosis of gonococcal infection. J Clin Microbiol. 1993 Jan;31(1):107–110. doi: 10.1128/jcm.31.1.107-110.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wiesner P. J. Gonococcal pharyngeal infection. Clin Obstet Gynecol. 1975 Mar;18(1):121–129. doi: 10.1097/00003081-197503000-00011. [DOI] [PubMed] [Google Scholar]

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