Abstract
Two amplification steps were made to detect Pneumocystis carinii DNA by polymerase chain reaction (PCR). pAZ102-E and pAZ102-H (standard PCR), pAZ102-L2 (sense), and pAZ102-E (antisense) (two-step PCR) were used as primers. The amplification products were analyzed by ethidium bromide. After the two-step PCR, ethidium bromide detected all samples positive by oligohybridization after one amplification step. Our two-step PCR is a rapid, cost-effective, and clinically suitable method for the detection of P. carinii infection.
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Selected References
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- Blumenfeld W., McCook O., Holodniy M., Katzenstein D. A. Correlation of morphologic diagnosis of Pneumocystis carinii with the presence of pneumocystis DNA amplified by the polymerase chain reaction. Mod Pathol. 1992 Mar;5(2):103–106. [PubMed] [Google Scholar]
- Lipschik G. Y., Gill V. J., Lundgren J. D., Andrawis V. A., Nelson N. A., Nielsen J. O., Ognibene F. P., Kovacs J. A. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet. 1992 Jul 25;340(8813):203–206. doi: 10.1016/0140-6736(92)90469-j. [DOI] [PubMed] [Google Scholar]
- Olsson M., Elvin K., Löfdahl S., Linder E. Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction. J Clin Microbiol. 1993 Feb;31(2):221–226. doi: 10.1128/jcm.31.2.221-226.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tamburrini E., Mencarini P., De Luca A., Maiuro G., Ventura G., Antinori A., Ammassari A., Visconti E., Ortona L., Siracusano A. Diagnosis of Pneumocystis carinii pneumonia: specificity and sensitivity of polymerase chain reaction in comparison with immunofluorescence in bronchoalveolar lavage specimens. J Med Microbiol. 1993 Jun;38(6):449–453. doi: 10.1099/00222615-38-6-449. [DOI] [PubMed] [Google Scholar]
- Wakefield A. E., Pixley F. J., Banerji S., Sinclair K., Miller R. F., Moxon E. R., Hopkin J. M. Detection of Pneumocystis carinii with DNA amplification. Lancet. 1990 Aug 25;336(8713):451–453. doi: 10.1016/0140-6736(90)92008-6. [DOI] [PubMed] [Google Scholar]