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. 1993 Oct;31(10):2816–2818. doi: 10.1128/jcm.31.10.2816-2818.1993

Use of the Quantitative Buffy Coat system for detection of parasitemia in patients with babesiosis.

A R Mattia 1, M A Waldron 1, L S Sierra 1
PMCID: PMC266026  PMID: 8253995

Abstract

Quantitative Buffy Coat analysis and blood smears were performed on a total of 47 blood samples. The technique showed 100% correlation with the blood smears in 9 samples containing babesia and 10 samples containing malaria, with some differential features distinguishing the two infections. Quantitative Buffy Coat analysis provides a simple and rapid method for the detection of parasitemia in cases of babesiosis.

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Selected References

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  1. Anthony R. L., Bangs M. J., Anthony J. M., Purnomo On-site diagnosis of Plasmodium falciparum, P. vivax, and P. malariae by using the Quantitative Buffy Coat system. J Parasitol. 1992 Dec;78(6):994–998. [PubMed] [Google Scholar]
  2. Bailey J. W., Smith D. H. The use of the acridine orange QBC technique in the diagnosis of African trypanosomiasis. Trans R Soc Trop Med Hyg. 1992 Nov-Dec;86(6):630–630. doi: 10.1016/0035-9203(92)90160-e. [DOI] [PubMed] [Google Scholar]
  3. Benezra D., Brown A. E., Polsky B., Gold J. W., Armstrong D. Babesiosis and infection with human immunodeficiency virus (HIV) Ann Intern Med. 1987 Dec;107(6):944–944. doi: 10.7326/0003-4819-107-6-944_1. [DOI] [PubMed] [Google Scholar]
  4. Brandt F., Healy G. R., Welch M. Human babesiosis: the isolation of Babesia microti in golden hamsters. J Parasitol. 1977 Oct;63(5):934–937. [PubMed] [Google Scholar]
  5. Chisholm E. S., Sulzer A. J., Ruebush T. K., 2nd Indirect immunofluorescence test for human Babesia microti infection: antigenic specificity. Am J Trop Med Hyg. 1986 Sep;35(5):921–925. doi: 10.4269/ajtmh.1986.35.921. [DOI] [PubMed] [Google Scholar]
  6. Dammin G. J., Spielman A., Benach J. L., Piesman J. The rising incidence of clinical Babesia microti infection. Hum Pathol. 1981 May;12(5):398–400. doi: 10.1016/s0046-8177(81)80020-2. [DOI] [PubMed] [Google Scholar]
  7. Golightly L. M., Hirschhorn L. R., Weller P. F. Fever and headache in a splenectomized woman. Rev Infect Dis. 1989 Jul-Aug;11(4):629–637. doi: 10.1093/clinids/11.4.629. [DOI] [PubMed] [Google Scholar]
  8. Gombert M. E., Goldstein E. J., Benach J. L., Tenenbaum M. J., Grunwaldt E., Kaplan M. H., Eveland L. K. Human babesiosis. Clinical and therapeutic considerations. JAMA. 1982 Dec 10;248(22):3005–3007. [PubMed] [Google Scholar]
  9. Gray A. D., Akin M. T., McLean R., Valeri C. R. Evaluation of the Quantitative Buffy Coat (QBC) method to detect malaria-infected red blood cells. Mil Med. 1991 May;156(5):241–245. [PubMed] [Google Scholar]
  10. Healy G. R., Ruebush T. K., 2nd Morphology of Babesia microti in human blood smears. Am J Clin Pathol. 1980 Jan;73(1):107–109. doi: 10.1093/ajcp/73.1.107. [DOI] [PubMed] [Google Scholar]
  11. Jacoby G. A., Hunt J. V., Kosinski K. S., Demirjian Z. N., Huggins C., Etkind P., Marcus L. C., Spielman A. Treatment of transfusion-transmitted babesiosis by exchange transfusion. N Engl J Med. 1980 Nov 6;303(19):1098–1100. doi: 10.1056/NEJM198011063031906. [DOI] [PubMed] [Google Scholar]
  12. Krause P. J., Telford S. R., 3rd, Pollack R. J., Ryan R., Brassard P., Zemel L., Spielman A. Babesiosis: an underdiagnosed disease of children. Pediatrics. 1992 Jun;89(6 Pt 1):1045–1048. [PubMed] [Google Scholar]
  13. Krause P. J., Telford S. R., 3rd, Ryan R., Hurta A. B., Kwasnik I., Luger S., Niederman J., Gerber M., Spielman A. Geographical and temporal distribution of babesial infection in Connecticut. J Clin Microbiol. 1991 Jan;29(1):1–4. doi: 10.1128/jcm.29.1.1-4.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Long G. W., Rickman L. S., Cross J. H. Rapid diagnosis of Brugia malayi and Wuchereria bancrofti filariasis by an acridine orange/microhematocrit tube technique. J Parasitol. 1990 Apr;76(2):278–281. [PubMed] [Google Scholar]
  15. Mattia A. R., Waldron M. A., Sierra L. S. Use of the UV ParaLens adapter as an alternative to conventional fluorescence microscopy for detection of Pneumocystis carinii in direct immunofluorescent monoclonal antibody-stained pulmonary specimens. J Clin Microbiol. 1993 Mar;31(3):720–721. doi: 10.1128/jcm.31.3.720-721.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Meldrum S. C., Birkhead G. S., White D. J., Benach J. L., Morse D. L. Human babesiosis in New York State: an epidemiological description of 136 cases. Clin Infect Dis. 1992 Dec;15(6):1019–1023. doi: 10.1093/clind/15.6.1019. [DOI] [PubMed] [Google Scholar]
  17. Mintz E. D., Anderson J. F., Cable R. G., Hadler J. L. Transfusion-transmitted babesiosis: a case report from a new endemic area. Transfusion. 1991 May;31(4):365–368. doi: 10.1046/j.1537-2995.1991.31491213305.x. [DOI] [PubMed] [Google Scholar]
  18. Persing D. H., Mathiesen D., Marshall W. F., Telford S. R., Spielman A., Thomford J. W., Conrad P. A. Detection of Babesia microti by polymerase chain reaction. J Clin Microbiol. 1992 Aug;30(8):2097–2103. doi: 10.1128/jcm.30.8.2097-2103.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Polsuwan C., Lumlertdaecha B., Tepsumethanon W., Wilde H. Using the UV ParaLens adapter on a standard laboratory microscope for fluorescent rabies antibody detection. Trans R Soc Trop Med Hyg. 1992 Jan-Feb;86(1):107–107. doi: 10.1016/0035-9203(92)90464-n. [DOI] [PubMed] [Google Scholar]
  20. Popovsky M. A., Lindberg L. E., Syrek A. L., Page P. L. Prevalence of Babesia antibody in a selected blood donor population. Transfusion. 1988 Jan-Feb;28(1):59–61. doi: 10.1046/j.1537-2995.1988.28188127955.x. [DOI] [PubMed] [Google Scholar]
  21. Rickman L. S., Long G. W., Oberst R., Cabanban A., Sangalang R., Smith J. I., Chulay J. D., Hoffman S. L. Rapid diagnosis of malaria by acridine orange staining of centrifuged parasites. Lancet. 1989 Jan 14;1(8629):68–71. doi: 10.1016/s0140-6736(89)91428-1. [DOI] [PubMed] [Google Scholar]
  22. Ruebush T. K., 2nd, Juranek D. D., Chisholm E. S., Snow P. C., Healy G. R., Sulzer A. J. Human babesiosis on Nantucket Island. Evidence for self-limited and subclinical infections. N Engl J Med. 1977 Oct 13;297(15):825–827. doi: 10.1056/NEJM197710132971511. [DOI] [PubMed] [Google Scholar]
  23. Spielman A., Perrone J. B., Teklehaimanot A., Balcha F., Wardlaw S. C., Levine R. A. Malaria diagnosis by direct observation of centrifuged samples of blood. Am J Trop Med Hyg. 1988 Oct;39(4):337–342. doi: 10.4269/ajtmh.1988.39.337. [DOI] [PubMed] [Google Scholar]
  24. Spielman A., Wilson M. L., Levine J. F., Piesman J. Ecology of Ixodes dammini-borne human babesiosis and Lyme disease. Annu Rev Entomol. 1985;30:439–460. doi: 10.1146/annurev.en.30.010185.002255. [DOI] [PubMed] [Google Scholar]
  25. Steketee R. W., Eckman M. R., Burgess E. C., Kuritsky J. N., Dickerson J., Schell W. L., Godsey M. S., Jr, Davis J. P. Babesiosis in Wisconsin. A new focus of disease transmission. JAMA. 1985 May 10;253(18):2675–2678. doi: 10.1001/jama.253.18.2675. [DOI] [PubMed] [Google Scholar]
  26. Sun T., Tenenbaum M. J., Greenspan J., Teichberg S., Wang R. T., Degnan T., Kaplan M. H. Morphologic and clinical observations in human infection with Babesia microti. J Infect Dis. 1983 Aug;148(2):239–248. doi: 10.1093/infdis/148.2.239. [DOI] [PubMed] [Google Scholar]

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