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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Apr;25(4):746–747. doi: 10.1128/jcm.25.4.746-747.1987

Comparison of three rapid diagnostic techniques for detection of respiratory syncytial virus from nasal wash specimens.

T Chonmaitree, B J Bessette-Henderson, R E Hepler, H L Lucia
PMCID: PMC266076  PMID: 3553236

Abstract

We report results of three rapid tests for respiratory syncytial virus antigen detection. An immunofluorescence assay using commercial antibody and two commercial enzyme immunoassays (Ortho Diagnostics, Inc., Raritan, N.J., and Abbott Laboratories, North Chicago, Ill.) were applied to 199 nasal wash specimens. The Abbott enzyme immunoassay was the most sensitive technique, with a sensitivity of 93.8%. The specificities of the three techniques were comparable and greater than 95%. The availability of reliable rapid diagnostic techniques will allow for better care of infants with severe respiratory syncytial virus infection.

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Selected References

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  1. Bromberg K., Tannis G., Daidone B., Clarke L., Sierra M. F. Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture. J Clin Microbiol. 1985 Dec;22(6):1071–1072. doi: 10.1128/jcm.22.6.1071-1072.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chao R. K., Fishaut M., Schwartzman J. D., McIntosh K. Detection of respiratory syncytial virus in nasal secretions from infants by enzyme-linked immunosorbent assay. J Infect Dis. 1979 Apr;139(4):483–486. doi: 10.1093/infdis/139.4.483. [DOI] [PubMed] [Google Scholar]
  3. Flanders R. T., Lindsay P. D., Chairez R., Brawner T. A., Kumar M. L., Swenson P. D., Bromberg K. Evaluation of clinical specimens for the presence of respiratory syncytial virus antigens using an enzyme immunoassay. J Med Virol. 1986 May;19(1):1–9. doi: 10.1002/jmv.1890190102. [DOI] [PubMed] [Google Scholar]
  4. Hall C. B., Douglas R. G., Jr, Geiman J. M., Messner M. K. Nosocomial respiratory syncytial virus infections. N Engl J Med. 1975 Dec 25;293(26):1343–1346. doi: 10.1056/NEJM197512252932604. [DOI] [PubMed] [Google Scholar]
  5. Hall C. B., McBride J. T., Gala C. L., Hildreth S. W., Schnabel K. C. Ribavirin treatment of respiratory syncytial viral infection in infants with underlying cardiopulmonary disease. JAMA. 1985 Dec 6;254(21):3047–3051. [PubMed] [Google Scholar]
  6. Hall C. B., McBride J. T., Walsh E. E., Bell D. M., Gala C. L., Hildreth S., Ten Eyck L. G., Hall W. J. Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study. N Engl J Med. 1983 Jun 16;308(24):1443–1447. doi: 10.1056/NEJM198306163082403. [DOI] [PubMed] [Google Scholar]
  7. Lauer B. A., Masters H. A., Wren C. G., Levin M. J. Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. J Clin Microbiol. 1985 Nov;22(5):782–785. doi: 10.1128/jcm.22.5.782-785.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McIntosh K., Hendry R. M., Fahnestock M. L., Pierik L. T. Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples. J Clin Microbiol. 1982 Aug;16(2):329–333. doi: 10.1128/jcm.16.2.329-333.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mintz L., Ballard R. A., Sniderman S. H., Roth R. S., Drew W. L. Nosocomial respiratory syncytial virus infections in an intensive care nursery: rapid diagnosis by direct immunofluorescence. Pediatrics. 1979 Aug;64(2):149–153. [PubMed] [Google Scholar]
  10. Swenson P. D., Kaplan M. H. Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay. J Clin Microbiol. 1986 Mar;23(3):485–488. doi: 10.1128/jcm.23.3.485-488.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Taber L. H., Knight V., Gilbert B. E., McClung H. W., Wilson S. Z., Norton H. J., Thurson J. M., Gordon W. H., Atmar R. L., Schlaudt W. R. Ribavirin aerosol treatment of bronchiolitis associated with respiratory syncytial virus infection in infants. Pediatrics. 1983 Nov;72(5):613–618. [PubMed] [Google Scholar]

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