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. 1987 Apr;25(4):748–750. doi: 10.1128/jcm.25.4.748-750.1987

Differential immunoglobulin G subclass antibody titers to respiratory syncytial virus F and G glycoproteins in adults.

D K Wagner, D L Nelson, E E Walsh, C B Reimer, F W Henderson, B R Murphy
PMCID: PMC266077  PMID: 3571485

Abstract

Two respiratory syncytial virus glycoproteins, F and G, which differ substantially in the amount of glycosylation were used as antigens in an enzyme-linked immunosorbent assay to determine immunoglobulin G (IgG) subclass titers in 30 experimentally infected healthy adults. The titers of antibodies to the F glycoprotein achieved in postinfection sera were highest in the IgG1 subclass, whereas those to the G glycoprotein were highest and comparable in the IgG1 and IgG2 subclasses. The high IgG2 response to the G glycoprotein suggests that it is seen by the immune system as a polysaccharide antigen, a hypothesis consistent with its large carbohydrate content.

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Selected References

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  1. Coleman R. M., Nahmias A. J., Williams S. C., Phillips D. J., Black C. M., Reimer C. B. IgG subclass antibodies to herpes simplex virus. J Infect Dis. 1985 May;151(5):929–936. doi: 10.1093/infdis/151.5.929. [DOI] [PubMed] [Google Scholar]
  2. Collins P. L., Huang Y. T., Wertz G. W. Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7683–7687. doi: 10.1073/pnas.81.24.7683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dall'Olio F., Malagolini N., Speziali V., Campadelli-Fiume G., Serafini-Cessi F. Sialylated oligosaccharides O-glycosidically linked to glycoprotein C from herpes simplex virus type 1. J Virol. 1985 Oct;56(1):127–134. doi: 10.1128/jvi.56.1.127-134.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Elango N., Satake M., Coligan J. E., Norrby E., Camargo E., Venkatesan S. Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus of F1 subunit. Nucleic Acids Res. 1985 Mar 11;13(5):1559–1574. doi: 10.1093/nar/13.5.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fernie B. F., Gerin J. L. Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion. Infect Immun. 1982 Jul;37(1):243–249. doi: 10.1128/iai.37.1.243-249.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hammarström L., Granström M., Oxelius V., Persson M. A., Smith C. I. IgG subclass distribution of antibodies against S. aureus teichoic acid and alpha-toxin in normal and immunodeficient donors. Clin Exp Immunol. 1984 Mar;55(3):593–601. [PMC free article] [PubMed] [Google Scholar]
  7. Hornsleth A., Bech-Thomsen N., Friis B. Detection by ELISA of IgG-subclass-specific antibodies in primary respiratory syncytial (RS) virus infections. J Med Virol. 1985 Aug;16(4):321–328. doi: 10.1002/jmv.1890160404. [DOI] [PubMed] [Google Scholar]
  8. Julkunen I., Hovi T., Seppälä I., Mäkelä O. Immunoglobulin G subclass antibody responses in influenza A and parainfluenza type 1 virus infections. Clin Exp Immunol. 1985 Apr;60(1):130–138. [PMC free article] [PubMed] [Google Scholar]
  9. Keil W., Geyer R., Dabrowski J., Dabrowski U., Niemann H., Stirm S., Klenk H. D. Carbohydrates of influenza virus. Structural elucidation of the individual glycans of the FPV hemagglutinin by two-dimensional 1H n.m.r. and methylation analysis. EMBO J. 1985 Oct;4(10):2711–2720. doi: 10.1002/j.1460-2075.1985.tb03991.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Linde G. A., Hammarström L., Persson M. A., Smith C. I., Sundqvist V. A., Wahren B. Virus-specific antibody activity of different subclasses of immunoglobulins G and A in cytomegalovirus infections. Infect Immun. 1983 Oct;42(1):237–244. doi: 10.1128/iai.42.1.237-244.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Linde G. A. Subclass distribution of rubella virus-specific immunoglobulin G. J Clin Microbiol. 1985 Jan;21(1):117–121. doi: 10.1128/jcm.21.1.117-121.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mills J., 5th, Van Kirk J. E., Wright P. F., Chanock R. M. Experimental respiratory syncytial virus infection of adults. Possible mechanisms of resistance to infection and illness. J Immunol. 1971 Jul;107(1):123–130. [PubMed] [Google Scholar]
  13. Morell A., Roth-Wicky B., Skvaril F. Immunoglobulin G subclass restriction of antibodies against hepatitis B surface antigen. Infect Immun. 1983 Feb;39(2):565–568. doi: 10.1128/iai.39.2.565-568.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Niemann H., Geyer R., Klenk H. D., Linder D., Stirm S., Wirth M. The carbohydrates of mouse hepatitis virus (MHV) A59: structures of the O-glycosidically linked oligosaccharides of glycoprotein E1. EMBO J. 1984 Mar;3(3):665–670. doi: 10.1002/j.1460-2075.1984.tb01864.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pabst H. F., Kreth H. W. Ontogeny of the immune response as a basis of childhood disease. J Pediatr. 1980 Oct;97(4):519–534. doi: 10.1016/s0022-3476(80)80003-5. [DOI] [PubMed] [Google Scholar]
  16. Sarnesto A., Ranta S., Vänänen P., Mäkelä O. Proportions of Ig classes and subclasses in rubella antibodies. Scand J Immunol. 1985 Mar;21(3):275–282. doi: 10.1111/j.1365-3083.1985.tb01430.x. [DOI] [PubMed] [Google Scholar]
  17. Satake M., Coligan J. E., Elango N., Norrby E., Venkatesan S. Respiratory syncytial virus envelope glycoprotein (G) has a novel structure. Nucleic Acids Res. 1985 Nov 11;13(21):7795–7812. doi: 10.1093/nar/13.21.7795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Siber G. R., Schur P. H., Aisenberg A. C., Weitzman S. A., Schiffman G. Correlation between serum IgG-2 concentrations and the antibody response to bacterial polysaccharide antigens. N Engl J Med. 1980 Jul 24;303(4):178–182. doi: 10.1056/NEJM198007243030402. [DOI] [PubMed] [Google Scholar]
  19. Spiegelberg H. L. Biological activities of immunoglobulins of different classes and subclasses. Adv Immunol. 1974;19(0):259–294. doi: 10.1016/s0065-2776(08)60254-0. [DOI] [PubMed] [Google Scholar]
  20. Sundqvist V. A., Linde A., Wahren B. Virus-specific immunoglobulin G subclasses in herpes simplex and varicella-zoster virus infections. J Clin Microbiol. 1984 Jul;20(1):94–98. doi: 10.1128/jcm.20.1.94-98.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wagner D. K., Clements M. L., Reimer C. B., Snyder M., Nelson D. L., Murphy B. R. Analysis of immunoglobulin G antibody responses after administration of live and inactivated influenza A vaccine indicates that nasal wash immunoglobulin G is a transudate from serum. J Clin Microbiol. 1987 Mar;25(3):559–562. doi: 10.1128/jcm.25.3.559-562.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wagner D. K., Graham B. S., Wright P. F., Walsh E. E., Kim H. W., Reimer C. B., Nelson D. L., Chanock R. M., Murphy B. R. Serum immunoglobulin G antibody subclass responses to respiratory syncytial virus F and G glycoproteins after primary infection. J Clin Microbiol. 1986 Aug;24(2):304–306. doi: 10.1128/jcm.24.2.304-306.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Walsh E. E., Brandriss M. W., Schlesinger J. J. Purification and characterization of the respiratory syncytial virus fusion protein. J Gen Virol. 1985 Mar;66(Pt 3):409–415. doi: 10.1099/0022-1317-66-3-409. [DOI] [PubMed] [Google Scholar]
  24. Walsh E. E., Schlesinger J. J., Brandriss M. W. Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus. J Gen Virol. 1984 Apr;65(Pt 4):761–767. doi: 10.1099/0022-1317-65-4-761. [DOI] [PubMed] [Google Scholar]
  25. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S., Ball L. A. Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4075–4079. doi: 10.1073/pnas.82.12.4075. [DOI] [PMC free article] [PubMed] [Google Scholar]

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