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. 1993 Nov;31(11):2882–2889. doi: 10.1128/jcm.31.11.2882-2889.1993

Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.

P Kirschner 1, B Springer 1, U Vogel 1, A Meier 1, A Wrede 1, M Kiekenbeck 1, F C Bange 1, E C Böttger 1
PMCID: PMC266149  PMID: 7505291

Abstract

Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.

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Selected References

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