Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 Jan;26(1):34–37. doi: 10.1128/jcm.26.1.34-37.1988

Effects of amines and polyamines on turbidimetric and lysoplate assays for lysozyme.

J W Jenzano 1, R L Lundblad 1
PMCID: PMC266175  PMID: 3343312

Abstract

The lysoplate and turbidimetric assays have often been used to measure lysozyme in biological fluids, such as blood and saliva. When the same purified lysozyme standard is used in both assays, results obtained with the lysoplate assay are much higher than those obtained for the same sample in the turbidimetric assay. It is likely, therefore, that other components in the biological fluid sample influence the expression of lysozyme activity in one or both assays to produce such divergent results. Certain amines or polyamines are found in various biological fluids and have the potential to influence the reactions in these two assay systems. It was the purpose of this study to incorporate selected amines and polyamines into purified lysozyme preparations and saliva or into the assay preparations to compare their effects on observed lysozyme activity between the two assay systems. Results showed that when the amine or polyamine was included in the purified sample, lysozyme activity was considerably greater than that of controls in the lysoplate assay, whereas a negligible effect was observed in the turbidimetric assay. If the amine or polyamine was incorporated into the assay preparation, results were more dramatic, with large increases in activity in the lysoplate assay and concomitant inhibition in the turbidimetric assay. Similar effects were observed in the assay of lysozyme in saliva, suggesting a potential mechanism by which the two assay systems produce markedly different results for the same sample.

Full text

PDF
34

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brandtzaeg P. The 'Lyso-Plate' method for quantification of muramidase activity. Scand J Dent Res. 1972;80(2):166–167. doi: 10.1111/j.1600-0722.1972.tb00278.x. [DOI] [PubMed] [Google Scholar]
  2. Deonier R. C., Williams J. W. Self-association of muramidase (lysozyme) in solution at 25 degrees, pH 7.0, and I = 0.20. Biochemistry. 1970 Oct 27;9(22):4260–4267. doi: 10.1021/bi00824a004. [DOI] [PubMed] [Google Scholar]
  3. Greenwald R. A., Moy W. W. Effect of agarose variability on the measurement of lysozyme activity. Clin Chim Acta. 1976 Dec 1;73(2):299–305. doi: 10.1016/0009-8981(76)90176-5. [DOI] [PubMed] [Google Scholar]
  4. Hyatt A. T., Hayes M. L. Free amino acids and amines in human dental plaque. Arch Oral Biol. 1975 Mar;20(3):203–209. doi: 10.1016/0003-9969(75)90010-2. [DOI] [PubMed] [Google Scholar]
  5. Janssen P. T., van Bijsterveld O. P. Immunochemical determination of human tear lysozyme (muramidase) in keratoconjunctivitis sicca. Clin Chim Acta. 1982 May 20;121(2):251–260. doi: 10.1016/0009-8981(82)90066-3. [DOI] [PubMed] [Google Scholar]
  6. Jenzano J. W., Hogan S. L., Lundblad R. L. Factors influencing measurement of human salivary lysozyme in lysoplate and turbidimetric assays. J Clin Microbiol. 1986 Dec;24(6):963–967. doi: 10.1128/jcm.24.6.963-967.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klass H. J., Hopkins J., Neale G., Peters T. J. The estimation of serum lysozyme: a comparison of four assay methods. Biochem Med. 1977 Aug;18(1):52–57. doi: 10.1016/0006-2944(77)90048-5. [DOI] [PubMed] [Google Scholar]
  8. LITWACK G. Photometric determination of lysozyme activity. Proc Soc Exp Biol Med. 1955 Jul;89(3):401–403. doi: 10.3181/00379727-89-21824. [DOI] [PubMed] [Google Scholar]
  9. McEvoy F. A., Hartley C. B. Polyamines in cystic fibrosis. Pediatr Res. 1975 Sep;9(9):721–724. doi: 10.1203/00006450-197509000-00007. [DOI] [PubMed] [Google Scholar]
  10. Modéer T., Söder P. O. A diffusion method for determination of lysozyme activity. Scand J Dent Res. 1971;79(7):533–535. doi: 10.1111/j.1600-0722.1971.tb02052.x. [DOI] [PubMed] [Google Scholar]
  11. Mörsky P. Turbidimetric determination of lysozyme with Micrococcus lysodeikticus cells: reexamination of reaction conditions. Anal Biochem. 1983 Jan;128(1):77–85. doi: 10.1016/0003-2697(83)90347-0. [DOI] [PubMed] [Google Scholar]
  12. Osserman E. F., Lawlor D. P. Serum and urinary lysozyme (muramidase) in monocytic and monomyelocytic leukemia. J Exp Med. 1966 Nov 1;124(5):921–952. doi: 10.1084/jem.124.5.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peters E. H., Azen E. A. Isolation and partial characterization of human parotid basic proteins. Biochem Genet. 1977 Oct;15(9-10):925–946. doi: 10.1007/BF00483989. [DOI] [PubMed] [Google Scholar]
  14. Ragetli H. W., Weintraub M. Agar derivatives for gel electrophoresis requiring reduced endosmotic liquid flow. Biochim Biophys Acta. 1966 Jan 4;112(1):160–167. doi: 10.1016/s0926-6585(96)90018-7. [DOI] [PubMed] [Google Scholar]
  15. SELA M., STEINER L. A. INHIBITION OF LYSOZYME BY SOME COPOLYMERS OF AMINO ACIDS. Biochemistry. 1963 May-Jun;2:416–421. doi: 10.1021/bi00903a004. [DOI] [PubMed] [Google Scholar]
  16. SHUGAR D. The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme. Biochim Biophys Acta. 1952 Mar;8(3):302–309. doi: 10.1016/0006-3002(52)90045-0. [DOI] [PubMed] [Google Scholar]
  17. STEIN W. H., MOORE S. The free amino acids of human blood plasma. J Biol Chem. 1954 Dec;211(2):915–926. [PubMed] [Google Scholar]
  18. Vasstrand E. N., Jensen H. B. Affinity chromatography of human saliva lysozyme and effect of pH and ionic strength on lytic activity. Scand J Dent Res. 1980 Jun;88(3):219–228. doi: 10.1111/j.1600-0722.1980.tb01218.x. [DOI] [PubMed] [Google Scholar]
  19. Vratsanos S. M., Mandel I. D. Polyamines of dental plaque in caries-resistant vs. caries-susceptible adults. J Dent Res. 1985 Mar;64(3):422–424. doi: 10.1177/00220345850640030501. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES