Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Apr;15(4):546–559. doi: 10.1261/rna.1194009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009 RNA Society

PMC Copyright notice

FIGURE 3. — Analysis of deduced telomeric repeats by Southern blot. (A) Chromosomal DNA samples from L. elongisporus CBS5301 (lane 1) and C. parapsilosis CBS2194 (lane 2), SR23 (lane 3), and CBS604^T (lane 4) were separated by pulsed-field gel electrophoresis and subjected to Southern blot analysis using a 5′-end-labeled oligonucleotide consisting of three copies of the putative C. parapsilosis telomeric repeat as a probe (Supplemental Table S1). Ethidium bromide staining of this gel is shown on the left and the autoradiogram on the right. (B) Southern blot analysis of C. parapsilosis SR23 telomeric restriction fragments obtained by digestion of gDNA with AluI (lane 1), Csp6I (lane 2), HaeIII (lane 3), HinPI (lane 4), MseI (lane 5), Sau3A (lane 6), TaqI (lane 7), MspI (lane 8), AvaII (lane 9), and EcoRI (lane 10). Note that AvaII and MspI have a recognition site within the C. parapsilosis telomeric repeat. (C) Southern blot analysis of telomeric DNA fragments obtained by digestion of gDNA from C. metapsilosis MCO448^T (lanes 1–4) and C. orthopsilosis MCO457^T (lanes 5–8) with AluI (lanes 1,5), Csp6I (lanes 2,6), MspI (lanes 3,7), and Sau3A (lanes 4,8). 5′-end-labeled oligonucleotides consisting of three copies of the putative telomeric repeats from C. metapsilosis (lanes 1–4) or C. orthopsilosis (lanes 5–8) were used as probes (Supplemental Table S1).