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. 1993 Nov;31(11):3050–3052. doi: 10.1128/jcm.31.11.3050-3052.1993

Growth in serum-free medium improves isolation of Chlamydia pneumoniae.

M Maass 1, A Essig 1, R Marre 1, W Henkel 1
PMCID: PMC266211  PMID: 8263198

Abstract

Infectivity titers were determined for eight Chlamydia pneumoniae strains simultaneously grown in serum-free and serum-supplemented cell culture media. Use of serum-free medium resulted in a 10- to 50-fold increase in the susceptibility of HL cells to chlamydial infection. Comparative primary isolation of a wild-type strain also produced higher inclusion counts in a serum-free environment. Serum-free cultivation is recommended to increase the efficiency of C. pneumoniae isolation from clinical material and to permit elementary body purification without interference caused by serum components.

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Selected References

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