Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 Feb;26(2):167–170. doi: 10.1128/jcm.26.2.167-170.1988

Multicenter comparative evaluation of two rapid microscopic methods and culture for detection of Chlamydia trachomatis in patient specimens.

R C Tilton 1, F N Judson 1, R C Barnes 1, R P Gruninger 1, R W Ryan 1, O Steingrimsson 1
PMCID: PMC266244  PMID: 3277994

Abstract

Four hundred and seventy-three men and women at high risk for sexually transmitted disease were tested for the presence of Chlamydia trachomatis in the urethra or the endocervix. Four groups were involved in this multicenter study of two direct fluorescent-antibody microscopy tests, Kallestad Pathfinder and Syva Microtrak, compared with culture techniques. Results from the test sites indicated that there was no significant difference overall in the sensitivity and specificity of the two test kits. However, there was some interlaboratory variation seen in the sensitivity of the microscopy, but little difference in the specificity. Either kit could be an effective screening method for C. trachomatis in high-risk populations.

Full text

PDF
167

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coudron P. E., Fedorko D. P., Dawson M. S., Kaplowitz L. G., Brookman R. R., Dalton H. P., Davis B. A. Detection of Chlamydia trachomatis in genital specimens by the Microtrak direct specimen test. Am J Clin Pathol. 1986 Jan;85(1):89–92. doi: 10.1093/ajcp/85.1.89. [DOI] [PubMed] [Google Scholar]
  2. Friis B., Kuo C. C., Wang S. P., Mordhorst C. H., Grayston J. T. Rapid diagnosis of Chlamydia trachomatis pneumonia in infants. Acta Pathol Microbiol Immunol Scand B. 1984 Jun;92(3):139–143. doi: 10.1111/j.1699-0463.1984.tb02808.x. [DOI] [PubMed] [Google Scholar]
  3. Fuster C. D., Neinstein L. S. Vaginal Chlamydia trachomatis prevalence in sexually abused prepurbertal girls. Pediatrics. 1987 Feb;79(2):235–238. [PubMed] [Google Scholar]
  4. Grayston J. T., Kuo C. C., Wang S. P., Altman J. A new Chlamydia psittaci strain, TWAR, isolated in acute respiratory tract infections. N Engl J Med. 1986 Jul 17;315(3):161–168. doi: 10.1056/NEJM198607173150305. [DOI] [PubMed] [Google Scholar]
  5. Hammerschlag M. R., Herrmann J. E., Cox P., Worku M., Laux R., Howard L. V. Enzyme immunoassay for diagnosis of neonatal chlamydial conjunctivitis. J Pediatr. 1985 Nov;107(5):741–743. doi: 10.1016/s0022-3476(85)80404-2. [DOI] [PubMed] [Google Scholar]
  6. Lipkin E. S., Moncada J. V., Shafer M. A., Wilson T. E., Schachter J. Comparison of monoclonal antibody staining and culture in diagnosing cervical chlamydial infection. J Clin Microbiol. 1986 Jan;23(1):114–117. doi: 10.1128/jcm.23.1.114-117.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Paisley J. W., Lauer B. A., Melinkovich P., Gitterman B. A., Feiten D. J., Berman S. Rapid diagnosis of Chlamydia trachomatis pneumonia in infants by direct immunofluorescence microscopy of nasopharyngeal secretions. J Pediatr. 1986 Oct;109(4):653–655. doi: 10.1016/s0022-3476(86)80232-3. [DOI] [PubMed] [Google Scholar]
  8. Rapoza P. A., Quinn T. C., Kiessling L. A., Green W. R., Taylor H. R. Assessment of neonatal conjunctivitis with a direct immunofluorescent monoclonal antibody stain for Chlamydia. JAMA. 1986 Jun 27;255(24):3369–3373. [PubMed] [Google Scholar]
  9. Ripa K. T., Mårdh P. A. Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells. J Clin Microbiol. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ryan R. W., Kwasnik I., Steingrimsson O., Gudmundsson J., Thorarinsson H., Tilton R. C. Rapid detection of Chlamydia trachomatis by an enzyme immunoassay method. Diagn Microbiol Infect Dis. 1986 Sep;5(3):225–234. doi: 10.1016/0732-8893(86)90006-4. [DOI] [PubMed] [Google Scholar]
  11. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]
  12. Tjiam K. H., van Eijk R. V., van Heijst B. Y., Tideman G. J., van Joost T., Stolz E., Michel M. F. Evaluation of the direct fluorescent antibody test for diagnosis of chlamydial infections. Eur J Clin Microbiol. 1985 Dec;4(6):548–552. doi: 10.1007/BF02013392. [DOI] [PubMed] [Google Scholar]
  13. Uyeda C. T., Welborn P., Ellison-Birang N., Shunk K., Tsaouse B. Rapid diagnosis of chlamydial infections with the MicroTrak direct test. J Clin Microbiol. 1984 Nov;20(5):948–950. doi: 10.1128/jcm.20.5.948-950.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES