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. 1988 Feb;26(2):323–327. doi: 10.1128/jcm.26.2.323-327.1988

Commercial identification systems often fail to identify Providencia stuartii.

G Cornaglia 1, B Dainelli 1, F Berlutti 1, M C Thaller 1
PMCID: PMC266276  PMID: 3277997

Abstract

We tested 145 clinical isolates in an attempt to evaluate some of the most widely used commercial identification systems in Europe in terms of their ability to identify Providencia strains. Two manual miniaturized systems (API 20E and Enterotube II) and three mechanized-automated systems (Cobas-Bact, Sceptor System, and Titertek-Enterobac-Rapid Automated System) were evaluated. Providencia alcalifaciens and Providencia rettgeri strains were correctly identified by all systems in all cases, and in most cases identification was achieved without the aid of supplementary tube tests. By contrast, Providencia stuartii was identified without the aid of supplementary tube tests for only 42.5% (API 20E), 37.5% (Enterotube), 68.7% (Sceptor), and 71.2% (Cobas-Bact) of the isolates. The overall misidentification rates were 16.3, 11.3, 11.3, and 10%, respectively. The Titertek-Enterobac-Rapid Automated System failed to identify only 1 of 80 strains (1.3%) and required supplementary tests in 2 other cases (2.5%). Since four of the multitest systems examined often failed to correctly identify P. stuartii, we conclude that supplementary conventional tube tests should always be used to distinguish this species from the other taxa of the Proteeae tribe.

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Selected References

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  1. Cornaglia G., Pompei R., Dainelli B., Satta G. In vitro activity of ciprofloxacin against aerobic bacteria isolated in a southern European hospital. Antimicrob Agents Chemother. 1987 Oct;31(10):1651–1655. doi: 10.1128/aac.31.10.1651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Farmer J. J., 3rd, Davis B. R., Hickman-Brenner F. W., McWhorter A., Huntley-Carter G. P., Asbury M. A., Riddle C., Wathen-Grady H. G., Elias C., Fanning G. R. Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens. J Clin Microbiol. 1985 Jan;21(1):46–76. doi: 10.1128/jcm.21.1.46-76.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hawkey P. M., Pedler S. J., Turner A. Comparative in vitro activity of semisynthetic penicillins against Proteeae. Antimicrob Agents Chemother. 1983 Apr;23(4):619–621. doi: 10.1128/aac.23.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hawkey P. M. Providencia stuartii: a review of a multiply antibiotic-resistant bacterium. J Antimicrob Chemother. 1984 Mar;13(3):209–226. doi: 10.1093/jac/13.3.209. [DOI] [PubMed] [Google Scholar]
  5. Hickman-Brenner F. W., Farmer J. J., 3rd, Steigerwalt A. G., Brenner D. J. Providencia rustigianii: a new species in the family Enterobacteriaceae formerly known as Providencia alcalifaciens biogroup 3. J Clin Microbiol. 1983 Jun;17(6):1057–1060. doi: 10.1128/jcm.17.6.1057-1060.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hickman F. W., Framer J. J., 3rd, Steigerwalt A. G., Brenner D. J. Unusual groups of Morganella ("Proteus") morganii isolated from clinical specimens: lysine-positive and ornithine-negative biogroups. J Clin Microbiol. 1980 Jul;12(1):88–94. doi: 10.1128/jcm.12.1.88-94.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hickman F. W., Steigerwalt A. G., Farmer J. J., 3rd, Brenner D. J. Identification of Proteus penneri sp. nov., formerly known as Proteus vulgaris indole negative or as Proteus vulgaris biogroup 1. J Clin Microbiol. 1982 Jun;15(6):1097–1102. doi: 10.1128/jcm.15.6.1097-1102.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klastersky J., Bogaerts A. M., Noterman J., van Laer E., Daneau D., Mouawad E. Infections caused by Providence bacilli. Scand J Infect Dis. 1974;6(2):153–160. doi: 10.3109/inf.1974.6.issue-2.09. [DOI] [PubMed] [Google Scholar]
  9. Kocka F. E., Srinivasan S., Mowjood M., Kantor H. S. Nosocomial multiply resistant Providencia stuartii: a long-term outbreak with multiple biotypes and serotypes at one hospital. J Clin Microbiol. 1980 Feb;11(2):167–169. doi: 10.1128/jcm.11.2.167-169.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lapage S. P., Bascomb S., Willcox W. R., Curtis M. A. Identification of bacteria by computer: general aspects and perspectives. J Gen Microbiol. 1973 Aug;77(2):273–290. doi: 10.1099/00221287-77-2-273. [DOI] [PubMed] [Google Scholar]
  11. McHale P. J., Walker F., Scully B., English L., Keane C. T. Providencia stuartii infections: a review of 117 cases over an eight year period. J Hosp Infect. 1981 Jun;2(2):155–165. doi: 10.1016/0195-6701(81)90024-4. [DOI] [PubMed] [Google Scholar]
  12. Penner J. L., Hinton N. A., Hennessy J. Biotypes of Proteus rettgeri. J Clin Microbiol. 1975 Feb;1(2):136–142. doi: 10.1128/jcm.1.2.136-142.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Penner J. L., Preston M. A., Hennessy J. N., Barton L. J., Goodbody M. M. Species differences in susceptibilities of Proteeae spp. to six cephalosporins and three aminoglycosides. Antimicrob Agents Chemother. 1982 Aug;22(2):218–221. doi: 10.1128/aac.22.2.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Prentice B., Robinson B. L. A review of Providencia bacteremia in a general hospital, with a comment on patterns of antimicrobial sensitivity and use. Can Med Assoc J. 1979 Sep 22;121(6):745–749. [PMC free article] [PubMed] [Google Scholar]
  15. Shlaes D. M., Currie C. A. Endemic gentamicin resistance R factors on a spinal cord injury unit. J Clin Microbiol. 1983 Aug;18(2):236–241. doi: 10.1128/jcm.18.2.236-241.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tomfohrde K. M., Rhoden D. L., Smith P. B., Balows A. Evaluation of the redesigned enterotube--a system for the identification of Enterobacteriaceae. Appl Microbiol. 1973 Feb;25(2):301–304. doi: 10.1128/am.25.2.301-304.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Warren J. W. Providencia stuartii: a common cause of antibiotic-resistant bacteriuria in patients with long-term indwelling catheters. Rev Infect Dis. 1986 Jan-Feb;8(1):61–67. doi: 10.1093/clinids/8.1.61. [DOI] [PubMed] [Google Scholar]

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