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. 1988 Feb;26(2):347–353. doi: 10.1128/jcm.26.2.347-353.1988

Evaluation of enzyme-linked immunosorbent assay and reversed passive hemagglutination for detection of Crimean-Congo hemorrhagic fever virus antigen.

A J Shepherd 1, R Swanepoel 1, D E Gill 1
PMCID: PMC266280  PMID: 3125221

Abstract

Enzyme-linked immunosorbent assay (ELISA) and a reversed passive hemagglutination (RPHA) test were evaluated for rapid detection of Crimean-Congo hemorrhagic fever (CCHF) virus antigens. Both RPHA and ELISA detected CCHF antigen in the brains of infant mice 2 to 3 days after infection, several days before the animals sickened and died. Antigen was also detected after 1 to 2 days in infected cell culture extracts and after 2 to 4 days in culture supernatant fluids. Both tests detected CCHF antigen at threshold values of approximately 2.5 log10 tissue culture infective doses per ml and were more sensitive than complement fixation, immunodiffusion, or immunofluorescence. In a comparative study on specimens from CCHF patients, virus was isolated from 38 of 49 sera and 23 of 28 patients. Antigen was detected in 20 of 49 sera (15 of 28 patients) by RPHA and in 29 of 49 sera (18 of 28 patients) by ELISA. Antigenemia was detected more frequently in fatal cases (9 of 11) than in nonfatal cases (9 of 17). Although the antigen detection assays offered a more rapid approach than infectivity assays for diagnosing CCHF, the latter test was more sensitive. The results suggest that RPHA and ELISA may be of use in rapid diagnosis of CCHF infection, particularly in severe cases, in which the danger of nosocomial spread is greatest.

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Selected References

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  1. Al-Nakib W., Lloyd G., El-Mekki A., Platt G., Beeson A., Southee T. Preliminary report on arbovirus-antibody prevalence among patients in Kuwait: evidence of Congo/Crimean virus infection. Trans R Soc Trop Med Hyg. 1984;78(4):474–476. doi: 10.1016/0035-9203(84)90065-8. [DOI] [PubMed] [Google Scholar]
  2. Al-Tikriti S. K., Al-Ani F., Jurji F. J., Tantawi H., Al-Moslih M., Al-Janabi N., Mahmud M. I., Al-Bana A., Habib H., Al-Munthri H. Congo/Crimean haemorrhagic fever in Iraq. Bull World Health Organ. 1981;59(1):85–90. [PMC free article] [PubMed] [Google Scholar]
  3. Blackburn N. K., Swanepoel R. An investigation of flavivirus infections of cattle in Zimbabwe Rhodesia with particular reference to Wesselsbron virus. J Hyg (Lond) 1980 Aug;85(1):1–33. doi: 10.1017/s0022172400027066. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burney M. I., Ghafoor A., Saleen M., Webb P. A., Casals J. Nosocomial outbreak of viral hemorrhagic fever caused by Crimean Hemorrhagic fever-Congo virus in Pakistan, January 1976. Am J Trop Med Hyg. 1980 Sep;29(5):941–947. doi: 10.4269/ajtmh.1980.29.941. [DOI] [PubMed] [Google Scholar]
  5. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  6. CLARKE D. H. FURTHER STUDIES ON ANTIGENIC RELATIONSHIPS AMONG THE VIRUSES OF THE GROUP B TICK-BORNE COMPLEX. Bull World Health Organ. 1964;31:45–56. [PMC free article] [PubMed] [Google Scholar]
  7. Donets M. A., Rezapkin G. V., Ivanov A. P., Tkachenko E. A. Immunosorbent assays for diagnosis of Crimean-Congo hemorrhagic fever (CCHF). Am J Trop Med Hyg. 1982 Jan;31(1):156–162. doi: 10.4269/ajtmh.1982.31.156. [DOI] [PubMed] [Google Scholar]
  8. Jahrling P. B., Niklasson B. S., McCormick J. B. Early diagnosis of human Lassa fever by ELISA detection of antigen and antibody. Lancet. 1985 Feb 2;1(8423):250–252. doi: 10.1016/s0140-6736(85)91029-3. [DOI] [PubMed] [Google Scholar]
  9. Joubert J. R., King J. B., Rossouw D. J., Cooper R. A nosocomial outbreak of Crimean-Congo haemorrhagic fever at Tygerberg Hospital. Part III. Clinical pathology and pathogenesis. S Afr Med J. 1985 Nov 9;68(10):722–728. [PubMed] [Google Scholar]
  10. Klisenko G. A., Gaidamovich S. Ia, Zarubinskii V. Ia, Lapina T. F., Meliev A. M. Ekspress-diagnostika krymskoi gemorragicheskoi likhoradki v reaktsii nepriamoi gemaggliutinatsii. Vopr Virusol. 1984 Sep-Oct;29(5):566–569. [PubMed] [Google Scholar]
  11. Klisenko G. A., Siddiki S. M., Gaidamovich SYa Early diagnosis of viraemia in some experimental arbovirus infections in mice. Acta Virol. 1981 Sep;25(5):322–325. [PubMed] [Google Scholar]
  12. Monath T. P., Hill L. J., Brown N. V., Cropp C. B., Schlesinger J. J., Saluzzo J. F., Wands J. R. Sensitive and specific monoclonal immunoassay for detecting yellow fever virus in laboratory and clinical specimens. J Clin Microbiol. 1986 Jan;23(1):129–134. doi: 10.1128/jcm.23.1.129-134.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Niklasson B. S., Jahrling P. B., Peters C. J. Detection of Lassa virus antigens and Lassa virus-specific immunoglobulins G and M by enzyme-linked immunosorbent assay. J Clin Microbiol. 1984 Aug;20(2):239–244. doi: 10.1128/jcm.20.2.239-244.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Niklasson B., Grandien M., Peters C. J., Gargan T. P., 2nd Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Jun;17(6):1026–1031. doi: 10.1128/jcm.17.6.1026-1031.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saluzzo J. F., Le Guenno B. Rapid diagnosis of human Crimean-Congo hemorrhagic fever and detection of the virus in naturally infected ticks. J Clin Microbiol. 1987 May;25(5):922–924. doi: 10.1128/jcm.25.5.922-924.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sartorelli A. C., Fischer D. S., Downs W. G. Use of sarcoma 180/TG to prepare hyperimmune ascitic fluid in the mouse. J Immunol. 1966 Apr;96(4):676–682. [PubMed] [Google Scholar]
  17. Shepherd A. J., Swanepoel R., Leman P. A., Shepherd S. P. Comparison of methods for isolation and titration of Crimean-Congo hemorrhagic fever virus. J Clin Microbiol. 1986 Oct;24(4):654–656. doi: 10.1128/jcm.24.4.654-656.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shepherd A. J., Swanepoel R., Shepherd S. P., Leman P. A., Blackburn N. K., Hallett A. F. A nosocomial outbreak of Crimean-Congo haemorrhagic fever at Tygerberg Hospital. Part V. Virological and serological observations. S Afr Med J. 1985 Nov 9;68(10):733–736. [PubMed] [Google Scholar]
  19. Smirnova S. E., Karavanov A. S. Detection of Crimean haemorrhagic fever virus antigen by solid phase enzyme immunosorbent assay. Acta Virol. 1985 Jan;29(1):87–90. [PubMed] [Google Scholar]
  20. Smith A. L., Tignor G. H., Mifune K., Motohashi T. Isolation and assay of rabies serogroup viruses in CER cells. Intervirology. 1977;8(2):92–99. doi: 10.1159/000148883. [DOI] [PubMed] [Google Scholar]
  21. Suleiman M. N., Muscat-Baron J. M., Harries J. R., Satti A. G., Platt G. S., Bowen E. T., Simpson D. I. Congo/Crimean haemorrhagic fever in Dubai. An outbreak at the Rashid Hospital. Lancet. 1980 Nov 1;2(8201):939–941. [PubMed] [Google Scholar]
  22. Swanepoel R., Shepherd A. J., Leman P. A., Shepherd S. P., McGillivray G. M., Erasmus M. J., Searle L. A., Gill D. E. Epidemiologic and clinical features of Crimean-Congo hemorrhagic fever in southern Africa. Am J Trop Med Hyg. 1987 Jan;36(1):120–132. doi: 10.4269/ajtmh.1987.36.120. [DOI] [PubMed] [Google Scholar]
  23. Swanepoel R., Struthers J. K., McGillivray G. M. Reversed passive hemagglutination and inhibition with Rift Valley fever and Crimean-Congo hemorrhagic fever viruses. Am J Trop Med Hyg. 1983 May;32(3):610–617. doi: 10.4269/ajtmh.1983.32.610. [DOI] [PubMed] [Google Scholar]
  24. Swanepoel R., Struthers J. K., Shepherd A. J., McGillivray G. M., Nel M. J., Jupp P. G. Crimean-congo hemorrhagic fever in South Africa. Am J Trop Med Hyg. 1983 Nov;32(6):1407–1415. doi: 10.4269/ajtmh.1983.32.1407. [DOI] [PubMed] [Google Scholar]
  25. Woodall J. P., Williams M. C., Simpson D. I. Congo virus: a hitherto undescribed virus occurring in Africa. II. Identification studies. East Afr Med J. 1967 Feb;44(2):93–98. [PubMed] [Google Scholar]
  26. Wulff H., Slenczka W., Gear J. H. Early detection of antigen and estimation of virus yield in specimens from patients with Marburg virus disease. Bull World Health Organ. 1978;56(4):633–639. [PMC free article] [PubMed] [Google Scholar]

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