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. 1988 Feb;26(2):377–379. doi: 10.1128/jcm.26.2.377-379.1988

Comparison of three enzyme-linked immunosorbent assays and a direct fluorescent-antibody test for detection of respiratory syncytial virus antigen.

L R Krilov 1, L Marcoux 1, H D Isenberg 1
PMCID: PMC266288  PMID: 3277999

Abstract

We prospectively evaluated three enzyme immunoassays (EIAs) and a direct fluorescent-antibody (DFA) test for respiratory syncytial virus detection. Of 90 specimens, 79% gave the same results in all four tests (30 positive and 41 negative) and 97% were in agreement in three of the four assays. The agreement between the direct fluorescent-antibody test and each enzyme immunoassay was greater than or equal to 86%.

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Selected References

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  1. Ahluwalia G., Embree J., McNicol P., Law B., Hammond G. W. Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay. J Clin Microbiol. 1987 May;25(5):763–767. doi: 10.1128/jcm.25.5.763-767.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bromberg K., Tannis G., Daidone B., Clarke L., Sierra M. F. Comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and HEp-2 cell culture. J Clin Microbiol. 1985 Dec;22(6):1071–1072. doi: 10.1128/jcm.22.6.1071-1072.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bromberg K., Tannis G., Daidone B., Clarke L., Sierra M. Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay. J Clin Microbiol. 1987 Feb;25(2):434–436. doi: 10.1128/jcm.25.2.434-436.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cheeseman S. H., Pierik L. T., Leombruno D., Spinos K. E., McIntosh K. Evaluation of a commercially available direct immunofluorescent staining reagent for the detection of respiratory syncytial virus in respiratory secretions. J Clin Microbiol. 1986 Jul;24(1):155–156. doi: 10.1128/jcm.24.1.155-156.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chonmaitree T., Bessette-Henderson B. J., Hepler R. E., Lucia H. L. Comparison of three rapid diagnostic techniques for detection of respiratory syncytial virus from nasal wash specimens. J Clin Microbiol. 1987 Apr;25(4):746–747. doi: 10.1128/jcm.25.4.746-747.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Flanders R. T., Lindsay P. D., Chairez R., Brawner T. A., Kumar M. L., Swenson P. D., Bromberg K. Evaluation of clinical specimens for the presence of respiratory syncytial virus antigens using an enzyme immunoassay. J Med Virol. 1986 May;19(1):1–9. doi: 10.1002/jmv.1890190102. [DOI] [PubMed] [Google Scholar]
  7. Freymuth F., Quibriac M., Petitjean J., Amiel M. L., Pothier P., Denis A., Duhamel J. F. Comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques. J Clin Microbiol. 1986 Dec;24(6):1013–1016. doi: 10.1128/jcm.24.6.1013-1016.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hall C. B., Douglas R. G., Jr Clinically useful method for the isolation of respiratory syncytial virus. J Infect Dis. 1975 Jan;131(1):1–5. doi: 10.1093/infdis/131.1.1. [DOI] [PubMed] [Google Scholar]
  9. Hall C. B., Douglas R. G., Jr, Geiman J. M., Messner M. K. Nosocomial respiratory syncytial virus infections. N Engl J Med. 1975 Dec 25;293(26):1343–1346. doi: 10.1056/NEJM197512252932604. [DOI] [PubMed] [Google Scholar]
  10. Kumar M. L., Super D. M., Lembo R. M., Thomas F. C., Prokay S. L. Diagnostic efficacy of two rapid tests for detection of respiratory syncytial virus antigen. J Clin Microbiol. 1987 May;25(5):873–875. doi: 10.1128/jcm.25.5.873-875.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Minnich L. L., Ray C. G. Comparison of direct and indirect immunofluorescence staining of clinical specimens for detection of respiratory syncytial virus antigen. J Clin Microbiol. 1982 May;15(5):969–970. doi: 10.1128/jcm.15.5.969-970.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Popow-Kraupp T., Kern G., Binder C., Tuma W., Kundi M., Kunz C. Detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay, indirect immunofluorescence, and virus isolation: a comparative study. J Med Virol. 1986 Jun;19(2):123–134. doi: 10.1002/jmv.1890190204. [DOI] [PubMed] [Google Scholar]
  13. Ray C. G., Minnich L. L. Efficiency of immunofluorescence for rapid detection of common respiratory viruses. J Clin Microbiol. 1987 Feb;25(2):355–357. doi: 10.1128/jcm.25.2.355-357.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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