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. 1988 Mar;26(3):426–428. doi: 10.1128/jcm.26.3.426-428.1988

Serotyping of Clostridium difficile.

S Toma 1, G Lesiak 1, M Magus 1, H L Lo 1, M Delmée 1
PMCID: PMC266306  PMID: 2833528

Abstract

A total of 246 live Clostridium difficile cultures were serotyped by a slide agglutination technique. Fifteen grouping antisera were produced which serotyped 98% of the cultures (241 of 246). Our results indicated that certain serogroups may have specific pathogenicity. Strains of serogroups A, G, H, K, S1, and S4 were cytotoxigenic and were isolated mainly from adult patients with pseudomembranous colitis or antibiotic-associated diarrhea. Nontoxigenic strains of serogroups D and Cd-5 were isolated mainly from asymptomatic neonates and small children. Some cross-reactions occurred among some strains of serogroups A, Cd-5, G, and K. These strains were further examined by analysis of protein profiles and restriction endonuclease patterns to elucidate their serology. Typing of C. difficile by using slide agglutination is a simple technique suitable for routine examination. Serogrouping may be a useful epidemiological marker and could help in elucidating the medical relevance of some C. difficile isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett J. G., Chang T. W., Gurwith M., Gorbach S. L., Onderdonk A. B. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. N Engl J Med. 1978 Mar 9;298(10):531–534. doi: 10.1056/NEJM197803092981003. [DOI] [PubMed] [Google Scholar]
  2. Delmee M., Homel M., Wauters G. Serogrouping of Clostridium difficile strains by slide agglutination. J Clin Microbiol. 1985 Mar;21(3):323–327. doi: 10.1128/jcm.21.3.323-327.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Delmée M., Laroche Y., Avesani V., Cornelis G. Comparison of serogrouping and polyacrylamide gel electrophoresis for typing Clostridium difficile. J Clin Microbiol. 1986 Dec;24(6):991–994. doi: 10.1128/jcm.24.6.991-994.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fekety R., Kim K. H., Brown D., Batts D. H., Cudmore M., Silva J., Jr Epidemiology of antibiotic-associated colitis; isolation of Clostridium difficile from the hospital environment. Am J Med. 1981 Apr;70(4):906–908. doi: 10.1016/0002-9343(81)90553-2. [DOI] [PubMed] [Google Scholar]
  5. Heard S. R., O'Farrell S., Holland D., Crook S., Barnett M. J., Tabaqchali S. The epidemiology of Clostridium difficile with use of a typing scheme: nosocomial acquisition and cross-infection among immunocompromised patients. J Infect Dis. 1986 Jan;153(1):159–162. doi: 10.1093/infdis/153.1.159. [DOI] [PubMed] [Google Scholar]
  6. Kim K. H., Fekety R., Batts D. H., Brown D., Cudmore M., Silva J., Jr, Waters D. Isolation of Clostridium difficile from the environment and contacts of patients with antibiotic-associated colitis. J Infect Dis. 1981 Jan;143(1):42–50. doi: 10.1093/infdis/143.1.42. [DOI] [PubMed] [Google Scholar]
  7. Kim K., DuPont H. L., Pickering L. K. Outbreaks of diarrhea associated with Clostridium difficile and its toxin in day-care centers: evidence of person-to-person spread. J Pediatr. 1983 Mar;102(3):376–382. doi: 10.1016/s0022-3476(83)80652-0. [DOI] [PubMed] [Google Scholar]
  8. Kliegman R. M., Hack M., Jones P., Fanaroff A. A. Epidemiologic study of necrotizing enterocolitis among low-birth-weight infants. Absence of identifiable risk factors. J Pediatr. 1982 Mar;100(3):440–444. doi: 10.1016/s0022-3476(82)80456-3. [DOI] [PubMed] [Google Scholar]
  9. Larson H. E., Barclay F. E., Honour P., Hill I. D. Epidemiology of Clostridium difficile in infants. J Infect Dis. 1982 Dec;146(6):727–733. doi: 10.1093/infdis/146.6.727. [DOI] [PubMed] [Google Scholar]
  10. Miller S. D., Koornhof H. J. Clostridium difficile colitis associated with the use of antineoplastic agents. Eur J Clin Microbiol. 1984 Feb;3(1):10–13. doi: 10.1007/BF02032807. [DOI] [PubMed] [Google Scholar]
  11. Milligan D. W., Kelly J. K. Pseudomembranous colitis in a leukaemia unit: a report of five fatal cases. J Clin Pathol. 1979 Dec;32(12):1237–1243. doi: 10.1136/jcp.32.12.1237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakamura S., Mikawa M., Nakashio S., Takabatake M., Okado I., Yamakawa K., Serikawa T., Okumura S., Nishida S. Isolation of Clostridium difficile from the feces and the antibody in sera of young and elderly adults. Microbiol Immunol. 1981;25(4):345–351. doi: 10.1111/j.1348-0421.1981.tb00036.x. [DOI] [PubMed] [Google Scholar]
  13. Savage A. M., Alford R. H. Nosocomial spread of Clostridium difficile. Infect Control. 1983 Jan-Feb;4(1):31–33. doi: 10.1017/s0195941700057623. [DOI] [PubMed] [Google Scholar]
  14. Tabaqchali S., Holland D., O'Farrell S., Silman R. Typing scheme for Clostridium difficile: its application in clinical and epidemiological studies. Lancet. 1984 Apr 28;1(8383):935–938. doi: 10.1016/s0140-6736(84)92392-4. [DOI] [PubMed] [Google Scholar]

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