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. 1988 Mar;26(3):459–463. doi: 10.1128/jcm.26.3.459-463.1988

Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen.

R Matthews 1, J Burnie 1
PMCID: PMC266313  PMID: 3281974

Abstract

A dot immunobinding assay based on the detection of the immunodominant 47-kilodalton (kDa) antigen of Candida albicans is described for the serological diagnosis of systemic candidiasis. It was compared with a reverse passive latex agglutination test and a dot immunobinding assay with total unfractionated hyperimmune serum to C. albicans. Use of the 47-kDa antigen-specific probe increased both the sensitivity and specificity of the assay system. Patients with systemic candidiasis were detected earlier in the course of the infection. The rate of detection of systemic C. albicans infections in neutropenic patients was 77% compared with 55% with total antibody in the dot immunobinding assay and 29% with the latex test. All three assay systems were positive in over 73% of infected patients who were not neutropenic. The 47-kDa antigen-specific probe was relatively specific to C. albicans. Antibody probes to the immunodominant antigens of other yeasts might be incorporated in the same dot immunobinding assay to detect systemic candidiasis caused by other species of yeasts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Au-Young J. K., Troy F. A., Goldstein E. Serologic analysis of antigen-specific reactivity in patients with systemic candidiasis. Diagn Microbiol Infect Dis. 1985 Sep;3(5):419–432. doi: 10.1016/0732-8893(85)90081-1. [DOI] [PubMed] [Google Scholar]
  2. Bailey J. W., Sada E., Brass C., Bennett J. E. Diagnosis of systemic candidiasis by latex agglutination for serum antigen. J Clin Microbiol. 1985 May;21(5):749–752. doi: 10.1128/jcm.21.5.749-752.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burnie J. P., Matthews R. C., Fox A., Tabaqchali S. Use of immunoblotting to identify antigenic differences between the yeast and mycelial phases of Candida albicans. J Clin Pathol. 1985 Jun;38(6):701–706. doi: 10.1136/jcp.38.6.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burnie J. A reverse passive latex agglutination test for the diagnosis of systemic candidosis. J Immunol Methods. 1985 Oct 10;82(2):267–280. doi: 10.1016/0022-1759(85)90359-x. [DOI] [PubMed] [Google Scholar]
  5. Gentry L. O., Wilkinson I. D., Lea A. S., Price M. F. Latex agglutination test for detection of Candida antigen in patients with disseminated disease. Eur J Clin Microbiol. 1983 Apr;2(2):122–128. doi: 10.1007/BF02001577. [DOI] [PubMed] [Google Scholar]
  6. Gold J. W. Opportunistic fungal infections in patients with neoplastic disease. Am J Med. 1984 Mar;76(3):458–463. doi: 10.1016/0002-9343(84)90665-x. [DOI] [PubMed] [Google Scholar]
  7. Greenfield R. A., Jones J. M. Purification and characterization of a major cytoplasmic antigen of Candida albicans. Infect Immun. 1981 Nov;34(2):469–477. doi: 10.1128/iai.34.2.469-477.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kahn F. W., Jones J. M. Latex agglutination tests for detection of Candida antigens in sera of patients with invasive candidiasis. J Infect Dis. 1986 Mar;153(3):579–585. doi: 10.1093/infdis/153.3.579. [DOI] [PubMed] [Google Scholar]
  9. Matthews R. C., Burnie J. P., Tabaqchali S. Immunoblot analysis of the serological response in systemic candidosis. Lancet. 1984 Dec 22;2(8417-8418):1415–1418. doi: 10.1016/s0140-6736(84)91618-0. [DOI] [PubMed] [Google Scholar]
  10. Matthews R. C., Burnie J. P., Tabaqchali S. Isolation of immunodominant antigens from sera of patients with systemic candidiasis and characterization of serological response to Candida albicans. J Clin Microbiol. 1987 Feb;25(2):230–237. doi: 10.1128/jcm.25.2.230-237.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Meunier-Carpentier F., Kiehn T. E., Armstrong D. Fungemia in the immunocompromised host. Changing patterns, antigenemia, high mortality. Am J Med. 1981 Sep;71(3):363–370. doi: 10.1016/0002-9343(81)90162-5. [DOI] [PubMed] [Google Scholar]
  12. Neale T. J., Muir J. C., Drake B. The immunochemical characterisation of circulating immune complex constituents in Candida albicans osteomyelitis by isoelectric focusing, immunoblot, and immunoprint. Aust N Z J Med. 1987 Apr;17(2):201–209. doi: 10.1111/j.1445-5994.1987.tb00041.x. [DOI] [PubMed] [Google Scholar]
  13. Olmsted J. B. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples. J Biol Chem. 1981 Dec 10;256(23):11955–11957. [PubMed] [Google Scholar]
  14. Strockbine N. A., Largen M. T., Zweibel S. M., Buckley H. R. Identification and molecular weight characterization of antigens from Candida albicans that are recognized by human sera. Infect Immun. 1984 Feb;43(2):715–721. doi: 10.1128/iai.43.2.715-721.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Towbin H., Gordon J. Immunoblotting and dot immunobinding--current status and outlook. J Immunol Methods. 1984 Sep 4;72(2):313–340. doi: 10.1016/0022-1759(84)90001-2. [DOI] [PubMed] [Google Scholar]
  16. de Repentigny L., Reiss E. Current trends in immunodiagnosis of candidiasis and aspergillosis. Rev Infect Dis. 1984 May-Jun;6(3):301–312. doi: 10.1093/clinids/6.3.301. [DOI] [PubMed] [Google Scholar]

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