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. 1988 Apr;26(4):681–685. doi: 10.1128/jcm.26.4.681-685.1988

Gas-liquid chromatographic analysis of fatty acid methyl esters of Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae.

F P Canonica 1, M A Pisano 1
PMCID: PMC266410  PMID: 3366862

Abstract

Clinical isolates of Aeromonas hydrophila, A. sobria, and A. caviae whose fatty acid content had been analyzed by gas-liquid chromatography (GLC) displayed the following qualitatively similar GLC profiles: 12:0, 14:0, 15:0, 16:0, 17:0, 18:0, 16:1, 18:1, a-15:0, a-17:0, and 3-OH 14:0. The 16:0/17:0 area-percentage ratio separated the clinical aeromonads in accordance with their species designations. Aeromonads treated with subminimal inhibitory concentrations of the antibiotic cerulenin displayed the following altered qualitative fatty acid GLC profiles: 12:0, 14:0, 16:0, 18:0, 16:1, 18:1, and 3-OH 14:0. Cerulenin-treated cells failed to reproducibly display detectable levels of all odd-numbered-carbon-chain-length fatty acids observed in untreated cells. Cerulenin-treated cells also exhibited overall increases in 14:0 and 3-OH 14:0 and a decrease in total unsaturated fatty acid content.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brian B. L., Gardner E. W. A simple procedure for detecting the presence of cyclopropane fatty acids in bacterial lipids. Appl Microbiol. 1968 Apr;16(4):549–552. doi: 10.1128/am.16.4.549-552.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buttke T. M., Ingram L. O. Inhibition of unsaturated fatty acid synthesis in escherichia coli by the antibiotic cerulenin. Biochemistry. 1978 Nov 28;17(24):5282–5286. doi: 10.1021/bi00617a031. [DOI] [PubMed] [Google Scholar]
  3. Canonica F. P., Pisano M. A. Identification of hydroxy fatty acids in Aeromonas hydrophila, Aeromonas sobria, and Aeromonas caviae. J Clin Microbiol. 1985 Dec;22(6):1061–1062. doi: 10.1128/jcm.22.6.1061-1062.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dees S. B., Hollis D. G., Weaver R. E., Moss C. W. Cellular fatty acid composition of Pseudomonas marginata and closely associated bacteria. J Clin Microbiol. 1983 Nov;18(5):1073–1078. doi: 10.1128/jcm.18.5.1073-1078.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dees S. B., Karr D. E., Hollis D., Moss C. W. Cellular fatty acids of Capnocytophaga species. J Clin Microbiol. 1982 Nov;16(5):779–783. doi: 10.1128/jcm.16.5.779-783.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dees S. B., Moss C. W. Cellular fatty acids of Alcaligenes and Pseudomonas species isolated from clinical specimens. J Clin Microbiol. 1975 May;1(5):414–419. doi: 10.1128/jcm.1.5.414-419.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dees S. B., Moss C. W. Identification of Achromobacter species by cellular fatty acids and by production of keto acids. J Clin Microbiol. 1978 Jul;8(1):61–66. doi: 10.1128/jcm.8.1.61-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dees S. B., Moss C. W., Weaver R. E., Hollis D. Cellular fatty acid composition of Pseudomonas paucimobilis and groups IIk-2, Ve-1, and Ve-2. J Clin Microbiol. 1979 Aug;10(2):206–209. doi: 10.1128/jcm.10.2.206-209.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dees S. B., Powell J., Moss C. W., Hollis D. G., Weaver R. E. Cellular fatty acid composition of organisms frequently associated with human infections resulting from dog bites: Pasteurella multocida and groups of EF-4, IIj, M-5, and DF-2. J Clin Microbiol. 1981 Dec;14(6):612–616. doi: 10.1128/jcm.14.6.612-616.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Greenspan M. D., Alberts A. W., Vagelos P. R. Acyl carrier protein. 13. Beta-ketoacyl acyl carrier protein synthetase from Escherichia coli. J Biol Chem. 1969 Dec 10;244(23):6477–6485. [PubMed] [Google Scholar]
  11. Lambert M. A., Moss C. W. Comparison of the effects of acid and base hydrolyses on hydroxy and cyclopropane fatty acids in bacteria. J Clin Microbiol. 1983 Dec;18(6):1370–1377. doi: 10.1128/jcm.18.6.1370-1377.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lee J. V., Shread P., Furniss A. L., Bryant T. N. Taxonomy and description of Vibrio fluvialis sp. nov. (synonym group F vibrios, group EF6). J Appl Bacteriol. 1981 Feb;50(1):73–94. doi: 10.1111/j.1365-2672.1981.tb00873.x. [DOI] [PubMed] [Google Scholar]
  13. Moss C. W., Bibb W. F., Karr D. E., Guerrant G. O., Lambert M. A. Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov. J Clin Microbiol. 1983 Oct;18(4):917–919. doi: 10.1128/jcm.18.4.917-919.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Moss C. W., Dees S. B. Cellular fatty acid composition of WIGA, a rickettsia-like agent similar to the Legionnaires disease bacterium. J Clin Microbiol. 1979 Sep;10(3):390–391. doi: 10.1128/jcm.10.3.390-391.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Moss C. W., Dees S. B. Cellular fatty acids of Flavobacterium meningosepticum and Flavobacterium species group IIb. J Clin Microbiol. 1978 Dec;8(6):772–774. doi: 10.1128/jcm.8.6.772-774.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moss C. W., Dees S. B. Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids. J Chromatogr. 1975 Oct 29;112:594–604. doi: 10.1016/s0021-9673(00)99988-6. [DOI] [PubMed] [Google Scholar]
  17. Moss C. W., Kai A., Lambert M. A., Patton C. Isoprenoid quinone content and cellular fatty acid composition of Campylobacter species. J Clin Microbiol. 1984 Jun;19(6):772–776. doi: 10.1128/jcm.19.6.772-776.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moss C. W., Shinoda T., Samuels J. W. Determination of cellular fatty acid compositions of various yeasts by gas-liquid chromatography. J Clin Microbiol. 1982 Dec;16(6):1073–1079. doi: 10.1128/jcm.16.6.1073-1079.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. Cellular fatty acid composition of isolates from Legionnaires disease. J Clin Microbiol. 1977 Aug;6(2):140–143. doi: 10.1128/jcm.6.2.140-143.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Popoff M., Véron M. A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group. J Gen Microbiol. 1976 May;94(1):11–22. doi: 10.1099/00221287-94-1-11. [DOI] [PubMed] [Google Scholar]
  21. Rottem S., Markowitz O., Razin S. Cerulenin-induced changes in the lipopolysaccharide content and phospholipid composition of Proteus mirabilis. Eur J Biochem. 1978 Apr 17;85(2):451–456. doi: 10.1111/j.1432-1033.1978.tb12259.x. [DOI] [PubMed] [Google Scholar]
  22. Shaw D. H., Hodder H. J. Lipopolysaccharides of the motile aeromonads; core oligosaccharide analysis as an aid to taxonomic classification. Can J Microbiol. 1978 Jul;24(7):864–868. doi: 10.1139/m78-143. [DOI] [PubMed] [Google Scholar]
  23. Vance D., Goldberg I., Mitsuhashi O., Bloch K. Inhibition of fatty acid synthetases by the antibiotic cerulenin. Biochem Biophys Res Commun. 1972 Aug 7;48(3):649–656. doi: 10.1016/0006-291x(72)90397-x. [DOI] [PubMed] [Google Scholar]

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