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. 1993 Dec;31(12):3310–3313. doi: 10.1128/jcm.31.12.3310-3313.1993

Virological diagnosis of progressive multifocal leukoencephalopathy: detection of JC virus DNA in cerebrospinal fluid and brain tissue of AIDS patients.

H Moret 1, M Guichard 1, S Matheron 1, C Katlama 1, V Sazdovitch 1, J M Huraux 1, D Ingrand 1
PMCID: PMC266411  PMID: 8308125

Abstract

Cerebrospinal fluid (CSF) from 12 AIDS patients with clinical signs consistent with progressive multifocal leukoencephalopathy (PML) was examined by the polymerase chain reaction (PCR) for the presence of JC virus (JCV). A specific JCV target sequence was amplified in the CSF from 9 of the 12 patients and also in brain tissue from all nine JCV-positive patients. The clinical course, neuroimaging features, and, in four cases, histopathology of brain tissue proved that the nine patients had PML. In the other three patients with central nervous system disorders, the JCV genome was undetectable by PCR and Southern blot analysis in CSF and brain tissue. The clinical course and neuroimaging features ruled out PML in these three patients. Five CSF samples and five brain tissue specimens from 10 PML-free AIDS patients with central nervous system disorders were all negative for the JCV genome by PCR and Southern blot analysis. These results show that detection of JCV in CSF by PCR is a good alternative to brain tissue studies for the virological diagnosis of PML in AIDS patients.

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Selected References

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  1. Aksamit A. J., Gendelman H. E., Orenstein J. M., Pezeshkpour G. H. AIDS-associated progressive multifocal leukoencephalopathy (PML): comparison to non-AIDS PML with in situ hybridization and immunohistochemistry. Neurology. 1990 Jul;40(7):1073–1078. doi: 10.1212/wnl.40.7.1073. [DOI] [PubMed] [Google Scholar]
  2. Arthur R. R., Dagostin S., Shah K. V. Detection of BK virus and JC virus in urine and brain tissue by the polymerase chain reaction. J Clin Microbiol. 1989 Jun;27(6):1174–1179. doi: 10.1128/jcm.27.6.1174-1179.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berger J. R., Kaszovitz B., Post M. J., Dickinson G. Progressive multifocal leukoencephalopathy associated with human immunodeficiency virus infection. A review of the literature with a report of sixteen cases. Ann Intern Med. 1987 Jul;107(1):78–87. doi: 10.7326/0003-4819-107-1-78. [DOI] [PubMed] [Google Scholar]
  4. Gibson P. E., Gardner S. D., Field A. M. Use of a molecular probe for detecting JCV DNA directly in human brain material. J Med Virol. 1986 Jan;18(1):87–95. doi: 10.1002/jmv.1890180111. [DOI] [PubMed] [Google Scholar]
  5. Gillespie S. M., Chang Y., Lemp G., Arthur R., Buchbinder S., Steimle A., Baumgartner J., Rando T., Neal D., Rutherford G. Progressive multifocal leukoencephalopathy in persons infected with human immunodeficiency virus, San Francisco, 1981-1989. Ann Neurol. 1991 Oct;30(4):597–604. doi: 10.1002/ana.410300413. [DOI] [PubMed] [Google Scholar]
  6. Grinnell B. W., Padgett B. L., Walker D. L. Distribution of nonintegrated DNA from JC papovavirus in organs of patients with progressive multifocal leukoencephalopathy. J Infect Dis. 1983 Apr;147(4):669–675. doi: 10.1093/infdis/147.4.669. [DOI] [PubMed] [Google Scholar]
  7. Henson J., Rosenblum M., Armstrong D., Furneaux H. Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy. Neurology. 1991 Dec;41(12):1967–1971. doi: 10.1212/wnl.41.12.1967. [DOI] [PubMed] [Google Scholar]
  8. Karahalios D., Breit R., Dal Canto M. C., Levy R. M. Progressive multifocal leukoencephalopathy in patients with HIV infection: lack of impact of early diagnosis by stereotactic brain biopsy. J Acquir Immune Defic Syndr. 1992 Oct;5(10):1030–1038. [PubMed] [Google Scholar]
  9. Matsuda M., Jona M., Yasui K., Nagashima K. Genetic characterization of JC virus Tokyo-1 strain, a variant oncogenic in rodents. Virus Res. 1987 Apr;7(2):159–168. doi: 10.1016/0168-1702(87)90077-3. [DOI] [PubMed] [Google Scholar]
  10. Padgett B. L., Walker D. L., ZuRhein G. M., Eckroade R. J., Dessel B. H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. doi: 10.1016/s0140-6736(71)91777-6. [DOI] [PubMed] [Google Scholar]
  11. Rentier-Delrue F., Lubiniecki A., Howley P. M. Analysis of JC virus DNA purified directly from human progressive multifocal leukoencephalopathy brains. J Virol. 1981 May;38(2):761–769. doi: 10.1128/jvi.38.2.761-769.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stoner G. L., Soffer D., Ryschkewitsch C. F., Walker D. L., Webster H. D. A double-label method detects both early (T-antigen) and late (capsid) proteins of JC virus in progressive multifocal leukoencephalopathy brain tissue from AIDS and non-AIDS patients. J Neuroimmunol. 1988 Sep;19(3):223–236. doi: 10.1016/0165-5728(88)90004-5. [DOI] [PubMed] [Google Scholar]
  13. Telenti A., Aksamit A. J., Jr, Proper J., Smith T. F. Detection of JC virus DNA by polymerase chain reaction in patients with progressive multifocal leukoencephalopathy. J Infect Dis. 1990 Oct;162(4):858–861. doi: 10.1093/infdis/162.4.858. [DOI] [PubMed] [Google Scholar]
  14. Tornatore C., Berger J. R., Houff S. A., Curfman B., Meyers K., Winfield D., Major E. O. Detection of JC virus DNA in peripheral lymphocytes from patients with and without progressive multifocal leukoencephalopathy. Ann Neurol. 1992 Apr;31(4):454–462. doi: 10.1002/ana.410310426. [DOI] [PubMed] [Google Scholar]
  15. ZURHEIN G., CHOU S. M. PARTICLES RESEMBLING PAPOVA VIRUSES IN HUMAN CEREBRAL DEMYELINATING DISEASE. Science. 1965 Jun 11;148(3676):1477–1479. doi: 10.1126/science.148.3676.1477. [DOI] [PubMed] [Google Scholar]

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