Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Dec;31(12):3340–3343. doi: 10.1128/jcm.31.12.3340-3343.1993

Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments.

M Eyers 1, S Chapelle 1, G Van Camp 1, H Goossens 1, R De Wachter 1
PMCID: PMC266428  PMID: 7508460

Abstract

By comparing nucleic acid sequences determined for one of the most variable areas of 23S rRNA genes of 23 Campylobacter strains, we were able to identify regions specific for thermophilic Campylobacter strains. Oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase chain reaction. One primer pair selectively detected all thermophilic Campylobacter species, while four other primer pairs allowed discrimination among the thermophilic species Campylobacter coli, Campylobacter jejuni subsp. jejuni, Campylobacter lari, and Campylobacter upsaliensis. All primer sets were tested successfully on a large number of clinical isolates.

Full text

PDF

Images in this article

3342Image
on p.3342

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Camp R. C. Benchmarking--a conversation with Robert C. Camp. The source. Interview by Joe Flower. Healthc Forum J. 1993 Jan-Feb;36(1):30–36. [PubMed] [Google Scholar]
  2. De Rijk P., Neefs J. M., Van de Peer Y., De Wachter R. Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res. 1992 May 11;20 (Suppl):2075–2089. doi: 10.1093/nar/20.suppl.2075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Endtz H. P., Ruijs G. J., Zwinderman A. H., van der Reijden T., Biever M., Mouton R. P. Comparison of six media, including a semisolid agar, for the isolation of various Campylobacter species from stool specimens. J Clin Microbiol. 1991 May;29(5):1007–1010. doi: 10.1128/jcm.29.5.1007-1010.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gaydos C. A., Quinn T. C., Eiden J. J. Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene. J Clin Microbiol. 1992 Apr;30(4):796–800. doi: 10.1128/jcm.30.4.796-800.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Giesendorf B. A., Quint W. G., Henkens M. H., Stegeman H., Huf F. A., Niesters H. G. Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction. Appl Environ Microbiol. 1992 Dec;58(12):3804–3808. doi: 10.1128/aem.58.12.3804-3808.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goossens H., Pot B., Vlaes L., Van den Borre C., Van den Abbeele R., Van Naelten C., Levy J., Cogniau H., Marbehant P., Verhoef J. Characterization and description of "Campylobacter upsaliensis" isolated from human feces. J Clin Microbiol. 1990 May;28(5):1039–1046. doi: 10.1128/jcm.28.5.1039-1046.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goossens H., Vlaes L., De Boeck M., Pot B., Kersters K., Levy J., De Mol P., Butzler J. P., Vandamme P. Is "Campylobacter upsaliensis" an unrecognised cause of human diarrhoea? Lancet. 1990 Mar 10;335(8689):584–586. doi: 10.1016/0140-6736(90)90359-d. [DOI] [PubMed] [Google Scholar]
  8. Gumerlock P. H., Tang Y. J., Meyers F. J., Silva J., Jr Use of the polymerase chain reaction for the specific and direct detection of Clostridium difficile in human feces. Rev Infect Dis. 1991 Nov-Dec;13(6):1053–1060. doi: 10.1093/clinids/13.6.1053. [DOI] [PubMed] [Google Scholar]
  9. Hultman T., Bergh S., Moks T., Uhlén M. Bidirectional solid-phase sequencing of in vitro-amplified plasmid DNA. Biotechniques. 1991 Jan;10(1):84–93. [PubMed] [Google Scholar]
  10. Höpfl P., Ludwig W., Schleifer K. H., Larsen N. The 23S ribosomal RNA higher-order structure of Pseudomonas cepacia and other prokaryotes. Eur J Biochem. 1989 Nov 6;185(2):355–364. doi: 10.1111/j.1432-1033.1989.tb15123.x. [DOI] [PubMed] [Google Scholar]
  11. Mullis K. B., Faloona F. A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. doi: 10.1016/0076-6879(87)55023-6. [DOI] [PubMed] [Google Scholar]
  12. Oyofo B. A., Thornton S. A., Burr D. H., Trust T. J., Pavlovskis O. R., Guerry P. Specific detection of Campylobacter jejuni and Campylobacter coli by using polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2613–2619. doi: 10.1128/jcm.30.10.2613-2619.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Penner J. L. The genus Campylobacter: a decade of progress. Clin Microbiol Rev. 1988 Apr;1(2):157–172. doi: 10.1128/cmr.1.2.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  15. Taylor D. E., Eaton M., Yan W., Chang N. Genome maps of Campylobacter jejuni and Campylobacter coli. J Bacteriol. 1992 Apr;174(7):2332–2337. doi: 10.1128/jb.174.7.2332-2337.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taylor D. E., Hiratsuka K., Mueller L. Isolation and characterization of catalase-negative and catalase-weak strains of Campylobacter species, including "Campylobacter upsaliensis," from humans with gastroenteritis. J Clin Microbiol. 1989 Sep;27(9):2042–2045. doi: 10.1128/jcm.27.9.2042-2045.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Totten P. A., Patton C. M., Tenover F. C., Barrett T. J., Stamm W. E., Steigerwalt A. G., Lin J. Y., Holmes K. K., Brenner D. J. Prevalence and characterization of hippurate-negative Campylobacter jejuni in King County, Washington. J Clin Microbiol. 1987 Sep;25(9):1747–1752. doi: 10.1128/jcm.25.9.1747-1752.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Van Camp G., Chapelle S., De Wachter R. Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences. Curr Microbiol. 1993 Sep;27(3):147–151. doi: 10.1007/BF01576012. [DOI] [PubMed] [Google Scholar]
  19. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol. 1991 Jan;41(1):88–103. doi: 10.1099/00207713-41-1-88. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES