Abstract
We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.
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- Brawner D. L., Anderson G. L., Yuen K. Y. Serotype prevalence of Candida albicans from blood culture isolates. J Clin Microbiol. 1992 Jan;30(1):149–153. doi: 10.1128/jcm.30.1.149-153.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eisenstein B. I. The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis. N Engl J Med. 1990 Jan 18;322(3):178–183. doi: 10.1056/NEJM199001183220307. [DOI] [PubMed] [Google Scholar]
- Fukazawa Y. Antigenic structure of Candida albicans. Immunochemical basis of the serologic specificity of the mannans in yeasts. Immunol Ser. 1989;47:37–62. [PubMed] [Google Scholar]
- Miyakawa Y., Kagaya K., Fukazawa Y., Soe G. Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans. J Clin Microbiol. 1986 May;23(5):881–886. doi: 10.1128/jcm.23.5.881-886.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miyakawa Y., Kuribayashi T., Kagaya K., Suzuki M., Nakase T., Fukazawa Y. Role of specific determinants in mannan of Candida albicans serotype A in adherence to human buccal epithelial cells. Infect Immun. 1992 Jun;60(6):2493–2499. doi: 10.1128/iai.60.6.2493-2499.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miyakawa Y., Mabuchi T., Kagaya K., Fukazawa Y. Isolation and characterization of a species-specific DNA fragment for detection of Candida albicans by polymerase chain reaction. J Clin Microbiol. 1992 Apr;30(4):894–900. doi: 10.1128/jcm.30.4.894-900.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray P. R., Spizzo A. W., Niles A. C. Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system. J Clin Microbiol. 1991 May;29(5):901–905. doi: 10.1128/jcm.29.5.901-905.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pfaller M. A. The use of molecular techniques for epidemiologic typing of Candida species. Curr Top Med Mycol. 1992;4:43–63. doi: 10.1007/978-1-4612-2762-5_2. [DOI] [PubMed] [Google Scholar]
- Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
- Scherer S., Magee P. T. Genetics of Candida albicans. Microbiol Rev. 1990 Sep;54(3):226–241. doi: 10.1128/mr.54.3.226-241.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Soll D. R. High-frequency switching in Candida albicans. Clin Microbiol Rev. 1992 Apr;5(2):183–203. doi: 10.1128/cmr.5.2.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stevens D. A., Odds F. C., Scherer S. Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype. Rev Infect Dis. 1990 Mar-Apr;12(2):258–266. doi: 10.1093/clinids/12.2.258. [DOI] [PubMed] [Google Scholar]
- Suzuki T., Kobayashi I., Kanbe T., Tanaka K. High frequency variation of colony morphology and chromosome reorganization in the pathogenic yeast Candida albicans. J Gen Microbiol. 1989 Feb;135(Pt 2):425–434. doi: 10.1099/00221287-135-2-425. [DOI] [PubMed] [Google Scholar]
- Tarrand J. J., Guillot C., Wenglar M., Jackson J., Lajeunesse J. D., Rolston K. V. Clinical comparison of the resin-containing BACTEC 26 Plus and the Isolator 10 blood culturing systems. J Clin Microbiol. 1991 Oct;29(10):2245–2249. doi: 10.1128/jcm.29.10.2245-2249.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]