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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 May;26(5):827–830. doi: 10.1128/jcm.26.5.827-830.1988

Congo red as a fluorochrome for the rapid detection of fungi.

M Slifkin 1, R Cumbie 1
PMCID: PMC266468  PMID: 2454936

Abstract

Congo red may be applied as a fluorochrome to rapidly detect fungi in clinical specimens, tissue, and fungal culture preparations. This generally available stain is cost effective and simple to prepare. The stain may be prepared with potassium permanganate as a counterstain or with Formalin or glutaraldehyde as a fungicide.

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Selected References

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  1. Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annu Rev Microbiol. 1968;22:87–108. doi: 10.1146/annurev.mi.22.100168.000511. [DOI] [PubMed] [Google Scholar]
  2. Bernstein M. E., Howard H. M., Carroll G. C. Fluorescence microscopy of Douglas fir foliage epiflora. Can J Microbiol. 1973 Sep;19(9):1129–1130. doi: 10.1139/m73-179. [DOI] [PubMed] [Google Scholar]
  3. Cabib E. Molecular aspects of yeast morphogenesis. Annu Rev Microbiol. 1975;29:191–214. doi: 10.1146/annurev.mi.29.100175.001203. [DOI] [PubMed] [Google Scholar]
  4. DARKEN M. A. Absorption and transport of fluorescent brighteners by microorganisms. Appl Microbiol. 1962 Sep;10:387–393. doi: 10.1128/am.10.5.387-393.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farkas V. Biosynthesis of cell walls of fungi. Microbiol Rev. 1979 Jun;43(2):117–144. doi: 10.1128/mr.43.2.117-144.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KUPER S. W., MAY J. R. Detection of acid-fast organisms in tissue sections by fluorescence microscopy. J Pathol Bacteriol. 1960 Jan;79:59–68. doi: 10.1002/path.1700790108. [DOI] [PubMed] [Google Scholar]
  7. Monheit J. E., Cowan D. F., Moore D. G. Rapid detection of fungi in tissues using calcofluor white and fluorescence microscopy. Arch Pathol Lab Med. 1984 Aug;108(8):616–618. [PubMed] [Google Scholar]
  8. Monheit J. G., Brown G., Kott M. M., Schmidt W. A., Moore D. G. Calcofluor white detection of fungi in cytopathology. Am J Clin Pathol. 1986 Feb;85(2):222–225. doi: 10.1093/ajcp/85.2.222. [DOI] [PubMed] [Google Scholar]
  9. San-Blas G. The cell wall of fungal human pathogens: its possible role in host-parasite relationships. Mycopathologia. 1982 Sep 17;79(3):159–184. doi: 10.1007/BF01837196. [DOI] [PubMed] [Google Scholar]
  10. Sautter R. L., Kwee H. G. Calcofluor white stain for fungi. Am J Clin Pathol. 1987 Feb;87(2):295–296. doi: 10.1093/ajcp/87.2.295a. [DOI] [PubMed] [Google Scholar]
  11. Slifkin M., Bowers H. M., Jr Phialophora mutabilis endocarditis. Am J Clin Pathol. 1975 Jan;63(1):120–130. doi: 10.1093/ajcp/63.3.120. [DOI] [PubMed] [Google Scholar]
  12. TRUANT J. P., BRETT W. A., THOMAS W., Jr Fluorescence microscopy of tubercle bacilli stained with auramine and rhodamine. Henry Ford Hosp Med Bull. 1962 Jun;10:287–296. [PubMed] [Google Scholar]

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