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. 1988 May;26(5):842–845. doi: 10.1128/jcm.26.5.842-845.1988

Variation of electrophoretic karyotypes among clinical isolates of Candida albicans.

W G Merz 1, C Connelly 1, P Hieter 1
PMCID: PMC266471  PMID: 3290238

Abstract

Orthogonal-field-alternation gel electrophoresis was used to compare clinical isolates of Candida albicans by resolving chromosome-sized DNA molecules into an electrophoretic karyotype. Seven to nine bands were observed among isolates recovered from 17 patients. In addition, 14 distinct electrophoretic patterns were noted among the isolates from these patients. In a given individual, isolates were likely to have identical electrophoretic patterns. Therefore, the electrophoretic karyotype patterns demonstrated by orthogonal-field-alternation gel electrophoresis can be used to designate a strain for epidemiologic studies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carle G. F., Olson M. V. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. doi: 10.1093/nar/12.14.5647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kakar S. N., Partridge R. M., Magee P. T. A genetic analysis of Candida albicans: isolation of a wide variety of auxotrophs and demonstration of linkage and complementation. Genetics. 1983 Jun;104(2):241–255. doi: 10.1093/genetics/104.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kelly R., Miller S. M., Kurtz M. B., Kirsch D. R. Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants. Mol Cell Biol. 1987 Jan;7(1):199–208. doi: 10.1128/mcb.7.1.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lott T. J., Boiron P., Reiss E. An electrophoretic karyotype for Candida albicans reveals large chromosomes in multiples. Mol Gen Genet. 1987 Aug;209(1):170–174. doi: 10.1007/BF00329854. [DOI] [PubMed] [Google Scholar]
  5. Magee B. B., Magee P. T. Electrophoretic karyotypes and chromosome numbers in Candida species. J Gen Microbiol. 1987 Feb;133(2):425–430. doi: 10.1099/00221287-133-2-425. [DOI] [PubMed] [Google Scholar]
  6. McCreight M. C., Warnock D. W. Enhanced differentiation of isolates of Candida albicans using a modified resistogram method. Mykosen. 1982 Nov;25(11):589–598. doi: 10.1111/j.1439-0507.1982.tb01926.x. [DOI] [PubMed] [Google Scholar]
  7. McCreight M. C., Warnock D. W., Martin M. V. Resistogram typing of Candida albicans isolates from oral and cutaneous sites in irradiated patients. Sabouraudia. 1985 Dec;23(6):403–406. [PubMed] [Google Scholar]
  8. Odds F. C., Abbott A. B. A simple system for the presumptive identification of Candida albicans and differentiation of strains within the species. Sabouraudia. 1980 Dec;18(4):301–317. [PubMed] [Google Scholar]
  9. Odds F. C., Abbott A. B. Modification and extension of tests for differentiation of Candida species and strains. Sabouraudia. 1983 Mar;21(1):79–81. doi: 10.1080/00362178385380111. [DOI] [PubMed] [Google Scholar]
  10. Olaiya A. F., Sogin S. J. Ploidy determination of Canadida albicans. J Bacteriol. 1979 Dec;140(3):1043–1049. doi: 10.1128/jb.140.3.1043-1049.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Olivo P. D., McManus E. J., Riggsby W. S., Jones J. M. Mitochondrial DNA polymorphism in Candida albicans. J Infect Dis. 1987 Jul;156(1):214–215. doi: 10.1093/infdis/156.1.214. [DOI] [PubMed] [Google Scholar]
  12. Polonelli L., Archibusacci C., Sestito M., Morace G. Killer system: a simple method for differentiating Candida albicans strains. J Clin Microbiol. 1983 May;17(5):774–780. doi: 10.1128/jcm.17.5.774-780.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Poulter R. T., Rikkerink E. H. Genetic analysis of red, adenine-requiring mutants of Candida albicans. J Bacteriol. 1983 Dec;156(3):1066–1077. doi: 10.1128/jb.156.3.1066-1077.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Poulter R., Hanrahan V., Jeffery K., Markie D., Shepherd M. G., Sullivan P. A. Recombination analysis of naturally diploid Candida albicans. J Bacteriol. 1982 Dec;152(3):969–975. doi: 10.1128/jb.152.3.969-975.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Riggsby W. S., Torres-Bauza L. J., Wills J. W., Townes T. M. DNA content, kinetic complexity, and the ploidy question in Candida albicans. Mol Cell Biol. 1982 Jul;2(7):853–862. doi: 10.1128/mcb.2.7.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Román M. C., Linares Sicilia M. J. Preliminary investigation of Candida albicans biovars. J Clin Microbiol. 1983 Aug;18(2):430–431. doi: 10.1128/jcm.18.2.430-431.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sarachek A., Rhoads D. D., Schwarzhoff R. H. Hybridization of Candida albicans through fusion of protoplasts. Arch Microbiol. 1981 Mar;129(1):1–8. doi: 10.1007/BF00417169. [DOI] [PubMed] [Google Scholar]
  18. Scherer S., Stevens D. A. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987 Apr;25(4):675–679. doi: 10.1128/jcm.25.4.675-679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
  20. Snell R. G., Wilkins R. J. Separation of chromosomal DNA molecules from C.albicans by pulsed field gel electrophoresis. Nucleic Acids Res. 1986 Jun 11;14(11):4401–4406. doi: 10.1093/nar/14.11.4401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Suzuki T., Kanbe T., Kuroiwa T., Tanaka K. Occurrence of ploidy shift in a strain of the imperfect yeast Candida albicans. J Gen Microbiol. 1986 Feb;132(2):443–453. doi: 10.1099/00221287-132-2-443. [DOI] [PubMed] [Google Scholar]
  22. Vollrath D., Davis R. W. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields. Nucleic Acids Res. 1987 Oct 12;15(19):7865–7876. doi: 10.1093/nar/15.19.7865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Warnock D. W., Speller D. C., Day J. K., Farrell A. J. Resistogram method for differentiation of strains of Candida albicans. J Appl Bacteriol. 1979 Jun;46(3):571–578. doi: 10.1111/j.1365-2672.1979.tb00857.x. [DOI] [PubMed] [Google Scholar]
  24. Whelan W. L., Markie D. M., Simpkin K. G., Poulter R. M. Instability of Candida albicans hybrids. J Bacteriol. 1985 Mar;161(3):1131–1136. doi: 10.1128/jb.161.3.1131-1136.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Whelan W. L., Partridge R. M., Magee P. T. Heterozygosity and segregation in Candida albicans. Mol Gen Genet. 1980;180(1):107–113. doi: 10.1007/BF00267358. [DOI] [PubMed] [Google Scholar]
  26. Williamson M. I., Samaranayake L. P., MacFarlane T. W. Biotypes of oral Candida albicans and Candida tropicalis isolates. J Med Vet Mycol. 1986 Feb;24(1):81–84. [PubMed] [Google Scholar]
  27. de Jonge P., de Jongh F. C., Meijers R., Steensma H. Y., Scheffers W. A. Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts. Yeast. 1986 Sep;2(3):193–204. doi: 10.1002/yea.320020307. [DOI] [PubMed] [Google Scholar]

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