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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Pharmacol Ther. 2008 Dec 16;122(1):1–8. doi: 10.1016/j.pharmthera.2008.11.008

Functional Selectivity of EGF Family Peptide Growth Factors: Implications for Cancer

Kristy J Wilson 1, Jennifer L Gilmore 2, John Foley 2, Mark A Lemmon 3, David J Riese II 1
PMCID: PMC2665203  NIHMSID: NIHMS83332  PMID: 19135477

Abstract

Breast, prostate, pancreatic, colorectal, lung, and head and neck cancers exploit deregulated signaling by ErbB family receptors and their ligands, EOF family peptide growth factors. EGF family members that stimulate the same receptor are able to stimulate divergent biological responses both in cell culture and in vivo. This is analogous to the functional selectivity exhibited by ligands for G-protein coupled receptors. Here we review this literature and propose that this functional selectivity of EGF family members is due to distinctions in the conformation of the liganded receptor and subsequent differences in the sites of receptor tyrosine phosphorylation and receptor coupling to signaling effectors. We also discuss the roles of divergent ligand activity in establishing and maintaining malignant phenotypes. Finally, we discuss the potential of mutant EGF family ligands as cancer chemotherapeutics targeted to ErbB receptors.

Keywords: Epidermal Growth Factor Receptor, EGF, ErbB, receptors, ErbB4, signal transduction, Neuregulins, Transforming Growth Factor alpha, Amphiregulin

1. Introduction

Ligand functional selectivity is defined as divergent ligand activation of signaling pathways through a single receptor. Functionally selective ligands have been identified for the serotonin, opioid, dopamine, vasopressin, and adrenergic receptors (Urban et al., 2007). Ligand binding to these G-protein coupled receptors (GPCRs) results in receptor association with heterotrimeric G-proteins. Ligand functional selectivity appears to be mediated by distinctions in the conformation of liganded receptors and subsequent differential association of the liganded receptors with heterotrimeric G proteins (Urban et al., 2007). The plethora of functionally selective ligands for GPCRs has facilitated the elucidation of the mechanisms by which GPCRs couple to signaling effectors and biological responses. Moreover, functionally selective ligands portend the discovery of GPCR ligands that have therapeutic signaling properties but lack adverse signaling properties (Mailman, 2007).

Like many other receptor tyrosine kinases, ErbB family receptors have numerous peptide ligands encoded by several distinct genes and by alternatively-spliced transcripts. These peptide ligands exhibit differences in receptor affinity and display exquisite receptor binding specificity. Other factors contribute to ligand specificity, including distinctions in the timing of ligand expression, tissue-specific patterns of ligand expression and differences in post-translational cleavage and processing. Accessory molecules and co-receptors such as heparan sulfate proteoglycans may contribute to ligand specificity by sequestering local high concentrations of these growth factors or by controlling their bioavailability, thereby selectively modulating the duration and/or strength of signaling stimulation by those EGF family members that bind these molecules (Britsch, 2007; Citri & Yarden, 2006; Edwin et al., 2006; Higashiyama et al., 2008; Iwamoto & Mekada, 2006; Jones et al., 2003; Mahtouk et al., 2006; Nanba & Higashiyama, 2004; Nishi & Klagsbrun, 2004; Normanno et al., 2001; Ohtsu et al., 2006; Sanderson et al., 2006; Shilo, 2005; Singh & Harris, 2005).

In addition, peptide ligands that display binding for the same ErbB family receptor have recently been shown to exhibit differences in function. This review will discuss the functional selectivity of ErbB receptor ligands, particularly in the context of the roles that functionally-selective ErbB receptor ligands may play in human malignancies and in the context of the roles that functionally-selective ErbB receptor ligands may play in cancer drug discovery.

The ErbB family of receptor tyrosine kinases includes the epidermal growth factor (EOF) receptor (EGFR/ErbBl/HERl), ErbB2/HER2/Neu, ErbB3/HER3, and ErbB4/HER4. Signaling by ErbB receptors is of principal importance in the control of cell fate, influencing proliferation, survival, or differentiation; hence, deregulated signaling by these receptors plays important roles in human malignancies. Currently, both EGFR and ErbB2 are validated targets for cancer chemotherapeutics (Normanno & Gullick, 2006; Plosker & Keam, 2006) that are being used to treat breast, lung, colorectal, and head and neck cancers. However, the development of resistance to chemotherapeutic agents that target ErbB receptors (Blackball et al., 2006; Nahta et al., 2006) has spurred continuing investigation into the mechanisms by which ErbB family receptor signaling is regulated and may be deregulated. Combining multiple therapeutics that target ErbB receptors via independent mechanism of action can result in enhanced responses. Moreover, ErbB inhibitors with different mechanisms of action elicit therapeutic responses in tumors that are refractory to treatment with other therapeutics that target ErbB receptors (Cameron et al., in press; Geyer et al., 2006; Storniolo et al., 2005; Storniolo et al., 2008). Thus, identifying additional agents that can target ErbB family receptors through novel means is an important goal in the treatment of cancer.

Members of the EGF family of peptide growth factors serve as agonists for ErbB family receptors. They include EGF, transforming growth factor-alpha (TGFα), amphiregulin (AR), betacellulin (BTC), heparin-binding EGF-like growth factor (HB-EGF), epiregulin (EPR), epigen (EPG), and the neuregulins (NRGs) (Figure 1) (Kinugasa et al., 2004; Kochupurakkal et al., 2005; Normanno et al., 2005). Collectively, these agonists regulate the activity of the four ErbB family receptors, each of which appears to make a unique set of contributions to a complicated signaling network (Citri & Yarden, 2006). Tumor cell expression of some EGF family members, most notably TGFα, AR, and HB-EGF, is associated with poorer patient prognosis or resistance to chemotherapeutics (Celikel et al., 2007; Eckstein et al., 2008; Gee et al., 2005; Ishikawa et al., 2005; Ritter et al., 2007; Wang et al., 2007).

Figure 1. Amino acid sequence of the EGF homology domain of selected EGF family peptide growth factors.

Figure 1

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(A) Underlined are the six conserved cysteine residues that form the three intramolecular disulfide bridges present in the mature ligands. Selected EGF family peptide growth factors include EGF (NCBI Protein database # NP_001954), TGFα (#AAA61159), AR (#AAA51781), HB-EGF (AAA35956), BTC (#AAB25452), EPR (#BAA22146), EPG (#Q6UW88), NRG1α (#NP_039258), NRG1β (#ABR13844), NRG2α (#NP_004874), NRG2β (#NP_053584), NRG3 (#NP_001010848), NRG4 (#AAH17568), NRG5 (#BAA90820), and NRG6 (#AAC69612). NRG5 is also known as tomoregulin and NRG6 is also known as neuroglycan-C. (B) Carboxyl-terminal NRG2 residues that regulate differences in ligand potency, receptor affinity (residue 43), and intrinsic activity (residue 45) are noted.

Ligand binding causes the homo- and/or heterodimerization of ErbB receptors, leading to the activation of their intracellular tyrosine kinase domains. As a result, ligand binding causes ErbB receptor phosphorylation on cytoplasmic tyrosine residues. This provides a mechanism for coupling to downstream signaling proteins via Src homology-2 (SH2) and phosphotyrosine binding (PTB) domains, which both recognize specific sets of tyrosine phosphorylation sites (Citri & Yarden, 2006; Riese & Stern, 1998; Warren & Landgraf, 2006). The EGF family ligands exhibit a complex pattern of interactions with the four ErbB family receptors; for example, EGFR can bind eight different EGF family members (Figure 2) and Neuregulin 2beta (NRG2β) binds EGFR, ErbB3, and ErbB4 (Figure 2). Given that ErbB2 lacks a EGF family ligand, ErbB3 lacks kinase activity, and the four ErbB receptor display distinct patterns of coupling to signaling effectors (Citri & Yarden, 2006), differences in the affinity of a given EGF family member for each of the four ErbB receptors is a key determinant of ligand signaling specificity (Jones et al., 1999). Here we will review evidence from the literature indicating that specific ErbB ligands that stimulate a given ErbB receptor can direct different biological responses both in cell culture and in vivo. Next, we will propose a novel mechanism that may account for the divergent biological effects exhibited by EGFR and ErbB4 ligands. Finally, we will discuss evidence for this mechanism and discuss how distinctions in ligand activity might be exploited to develop a new class of cancer chemotherapeutics targeted to ErbB receptors.

Figure 2. EGF family ligands bind and activate multiple ErbB receptors.

Figure 2

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A Venn diagram illustrates the interactions of the four ErbB family receptors with EGF family members. This figure summarizes published data (Hobbs et al., 2002; Kinugasa et al., 2004; Kochupurakkal et al., 2005; Normanno et al., 2005).

2. EGF Family Ligands Stimulate Different Biological Outcomes From The Same Receptor

In a variety of cultured cell model systems, different EGF family ligands that bind the same receptor can promote divergent biological outcomes. Emerging data indicate that this is true even when the ligands are present at saturating concentrations. Thus, these distinctions in signaling are independent of ligand affinity or potency and appear to reflect differences in ligand intrinsic activity or efficacy. The EGFR ligands TGFα and AR stimulate equivalent levels of DNA synthesis in MDCK cells. AR also stimulates a morphologic change and redistribution of E-cadherin in these cells, but TGFα does not (Chung et al., 2005). In MCF10A human mammary epithelial cells, AR stimulates greater motility and invasiveness than does EGF (Willmarth & Ethier, 2006). Ectopic expression of EGFR in the 32D mouse myeloid cell line permits a saturating concentration of EGF to stimulate EGFR coupling to survival. In contrast, a saturating concentration of Neuregulin 2beta (NRG2β) stimulates EGFR coupling to proliferation in these cells (Gilmore et al., 2006). Finally, EGF, HB-EGF, and TGFα can suppress alcohol-induced apoptosis in human placental cytotrophoblast cells, whereas AR cannot (Wolff et al., 2007).

Different EGF family members can stimulate divergent biological outcomes from the same receptor in animal model systems. Transgenic mice in which AR is expressed in the epidermis from the K14 promoter lack hair follicles and exhibit epidermal hyperplasia, aberrant differentiation, resistance to apoptosis, and increased inflammation characterized by skin plaques (Cook et al., 2004; Cook et al., 1997). In contrast, transgenic mice in which TGFα is expressed from the K14 promoter exhibit only a thicker epidermis and stunted hair growth (Dominey et al., 1993; Vassar & Fuchs, 1991). Transgenic mice that lack AR exhibit more severe stunting of mammary gland outgrowth than do transgenic mice that lack EGF or TGFα. Indeed, AR appears to be the primary EGFR ligand involved in pubertal mammary ductal morphogenesis, whereas EGF and TGFα seem to play more pronounced roles in mammary gland morphogenesis during pregnancy and lactation (Booth & Smith, 2007; McBryan et al., 2008).

The in vitro and in vivo results discussed above are buttressed by emerging data indicating that the expression of specific EGFR ligands in certain tumors is differentially associated with prognosis. EGF expression in breast tumor samples is associated with a more favorable prognosis, whereas TGFα expression is associated with more aggressive tumors (Revillion et al., 2008). Likewise, microarray analyses reveal that early hyperplastic precursors of breast cancer display increased AR transcription and decreased EGF transcription relative to normal breast tissue (Lee et al., 2007). In non-small-cell lung carcinoma (NSCLC) patients, TGFα and AR serum concentrations correlate with tumor aggressiveness, but the serum concentration of EGF does not. In fact, the serum concentration of EGF is significantly higher in healthy individuals than in NSCLC patients (Lemos-Gonzalez et al., 2007). Moreover, NSCLC tumors that are refractory to the EGFR tyrosine kinase inhibitor gefitinib display increased TGFα and AR transcription than do tumors that are sensitive to gefitinib (Kakiuchi et al., 2004). Taken together, these data argue that TGFα and AR stimulate EGFR coupling to tumor cell aggressiveness and chemoresistance, while EGF fails to do so - and may in fact antagonize stimulation of pathogenic signaling by TGFα and AR.

Similarly, individual ErbB4 ligands appear to stimulate ErbB4 coupling to divergent biological responses. Ectopic expression of ErbB4 in the CEM human lymphoid cell line permits the ErbB4 ligands BTC, Neuregulin 1beta (NRG1β), Neuregulin 2beta (NRG2β), and Neuregulin 3 (NRG3) to stimulate similar levels of ErbB4 phosphorylation. However, in these CEM/ErbB4 cells BTC and NRG1 β stimulate greater viability and proliferation than do NRG2β and NRG3 (Sweeney et al., 2000). Ectopic expression of EGFR and ErbB4 in the BaF3 mouse lymphoid cell line permits the ErbB4 ligands NRG1β and NRG2β to stimulate proliferation. However, in these BaF3/EGFR+ErbB4 cells the ErbB4 ligand NRG3 stimulates survival, whereas the ErbB4 ligands Neuregulin 2alpha (NRG2α) and Neuregulin 4 (NRG4) fail to stimulate either survival or proliferation (Hobbs et al., 2002). Likewise, at the neuromuscular junction NRG2β appears to be more effective than NRG2α at stimulating ErbB4 coupling to increased transcription of the acetylcholine receptor (Ponomareva et al., 2005). These functional differences between NRG2α and NRG2β are particularly noteworthy given that these ligands are transcriptional splicing isoforms that differ in the carboxyl-terminus of the canonical EGF homology domain (Normanno et al., 2001; Normanno et al., 2005).

3. Mechanistic Model for Functionally Selective EGF Family Ligands

3.1. EGF family members differentially stimulate receptor coupling to signaling effectors by stimulating receptor phosphorylation on distinct sets of tyrosine residues

ErbB family receptors couple to a large variety of signaling effectors (Figure 3). We postulate that the complement of signaling effectors recruited to and activated by ligand-stimulated ErbB receptors is specified by the receptor and ligand, thereby accounting for differences in ligand intrinsic activity. For example, AR stimulates NF-κB signaling and Interleukin-1 (IL-1) secretion in MCF10A immortalized breast cells, but EGF does not. This appears to account for the divergent stimulation of motility and invasiveness by AR and EGF (Streicher et al., 2007). TGFα also appears to be functionally distinct from EGF. Both ligands stimulate migration and proliferation in fibroblasts; however, they appear to do so through different EGFR effectors. EGF stimulation of migration and proliferation requires p70s6k and CD44. In contrast, TGFα requires phospholipase Cγ (PLCγ) and integrin αvβ3 (Ellis et al., 2007).

Figure 3. Ligand stimulation of ErbB receptor tyrosine phosphorylation creates docking sites for numerous signaling effectors.

Figure 3

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Putative sites of EGFR, ErbB2, ErbB3, and ErbB4 tyrosine phosphorylation are denoted, as well as signaling effectors predicted or shown to bind to these sites of phosphorylation (Cohen et al., 1996; Hellyer et al., 2001; Kaushansky et al., 2008; Keilhack et al., 1998; Rotin et al., 1992; Schulze et al., 2005; Sorkin et al., 1996; Zrihan-Licht et al., 1998). The ErbB receptors are not drawn to scale.

Differences in the sites of ligand-induced EGFR tyrosine phosphorylation may underlie divergent ligand-induced EGFR coupling to signaling effectors and biological responses. For example, EGF stimulates abundant EGFR phosphorylation at Tyr1045 whereas AR does not (Gilmore et al., 2008). Phosphorylation of Tyr1045 creates a canonical binding site for the E3 ubiquitin ligase c-cbl, leading to EGFR ubiquitination and degradation by the 26S proteasome (Levkowitz et al., 1999; Levkowitz et al., 1998). Thus, unlike AR, EGF stimulates c-cbl binding to EGFR, EGFR ubiquitination, and EGFR degradation (Stern et al., 2008). We hypothesize that differential ligand-induced EGFR phosphorylation at Tyr1045 and differential EGFR coupling to cbl-dependent ubiquitination and turnover leads to distinctions in the duration of ligand-induced EGFR signaling, thereby accounting for the inability of EGF to stimulate motility and invasiveness in cells that do respond to AR.

Differences in the sites of ligand-induced receptor phosphorylation may also account for divergence in the intrinsic activity of ErbB4 ligands. The ErbB4 ligands BTC, NRG1β, NRG2β, and NRG3 differentially stimulate ErbB4 coupling to survival and proliferation in CEM/ErbB4 cells and differentially stimulate ErbB4 coupling to Shc, p85, Akt, and Erk1/2. This is accompanied by distinctions in the sites of ligand-induced ErbB4 tyrosine phosphorylation (Sweeney et al., 2000). NRG1β stimulates ErbB4 phosphorylation at nineteen tyrosine residues (Kaushansky et al., 2008); these residues are candidates for sites of ligand-specific tyrosine phosphorylation. However, ligand-specific sites of ErbB4 tyrosine phosphorylation have yet to be identified and the biological relevance of differences in these sites of ErbB4 phosphorylation has yet to be established.

3.2. Differences in the conformation of the liganded receptor extracellular domain may account for distinct patterns of ErbB receptor tyrosine phosphorylation and downstream signaling

The binding of an EGF family member to its cognate ErbB receptor stabilizes the receptor extracellular domains in an extended conformation that exposes a dimerization arm in subdomain II, thereby facilitating dimerization of the extracellular region (Burgess et al., 2003). This is thought to lead to asymmetric dimerization of the receptor intracellular domains, activation of kinase activity, and trans-phosphorylation of cytoplasmic tyrosine residues on one receptor monomer by the kinase domain of the other receptor monomer (Zhang et al., 2006).

We hypothesize that different ErbB ligands could stabilize the extracellular regions of a given ErbB receptor in subtly distinct conformations. This could alter the details of the interaction between the two intracellular domains in the observed asymmetric dimer, in turn influencing which cytoplasmic tyrosine residues of each receptor monomer are most efficiently phosphorylated by their dimerization partner. Conformationally-distinct dimers might possess different complements of phosphorylated tyrosine residues, leading to activation of unique sets of signaling effectors and distinct biological outcomes (Figure 4). Studies using constitutively active ErbB2 and ErbB4 mutants reveal that artificially manipulating the structural relationship between two receptor monomers within a dimer can result in divergent receptor signaling and coupling to downstream events (Burke & Stern, 1998; Pitfield et al., 2006; Williams et al., 2003). In addition, evidence for ligand-specific receptor conformations can be seen in a comparison of the EGFR extracellular region bound to EGF or TGFα. The conformation of the EGFR extracellular subdomain II is subtly different in the EGFR-EGF and EGFR-TGFα complexes (Figure 5) (Garrett et al., 2002; Harte & Gentry, 1995; Ogiso et al., 2002). Since the dimerization interface extends along this entire domain (Dawson et al., 2005), it appears reasonable to suggest that EGF and TGFα promote different spatial relationships between the EGFR monomers within a receptor dimer. It has been postulated that ligand-specific alterations in the conformation of subdomain II may reflect ligand-specific differences in the buttressing of subdomain II by the EGFR extracellular subdomain III (Dawson et al., 2005). The plausibility of this hypothesis is supported by the fact that the EGFR extracellular subdomain III contains one of the two sites for binding EGF family ligands and is therefore a reasonable potential site for ligand-specific conformational changes. Further efforts are required to fully understand the proximal (the structure of receptors bound to functionally selective ligands), intermediate (sites of differential receptor phosphorylation caused by functionally selective ligands), and distal mechanisms (distinctions in signaling pathway activation and gene expression) that underlie the functional selectivity of EGF family ligands.

Figure 4. Model for differential ligand-induced ErbB receptor coupling to biological responses.

Figure 4

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(A) Hypothetical depiction of differences in the conformation of receptor extracellular and intracellular domains and distinctions in the sites of receptor tyrosine phosphorylation following ligand binding. (B) Hypothetical depiction of the difference in the juxtapositioning of receptor extracellular domain monomers within a ligand-induced dimer. (C) Depiction of the distinctions in EGF and AR stimulation of EGFR phosphorylation at Tyr1045, resulting in differences in the binding of c-cbl, alterations in signaling duration, and changes in the biological consequences of EGFR signaling (Gilmore et al., 2008; Stern et al., 2008; Willmarth & Ethier, 2006).

Figure 5. Differences in the conformation of the EGFR extracellular domain following EGF or TGFα binding.

Figure 5

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(A) A worm representation of an EGFR extracellular domain dimer, with subdomain II colored green at the dimer interface. (B) A worm representation of EGFR extracellular subdomain II conformation following binding of EGF (green) or TGFα (blue). Subtle differences, particularly at the upper site of dimerization, are marked with a star.

4. Implications of Functionally Selective EGF Family Ligands

4.1. Analyses of the roles that EGF family ligands and ErbB receptors play in tumorigenesis and tumor progression must account for differences in the intrinsic activity of EGF family members

Many studies of ligand-induced ErbB signaling in tumor cell model systems have employed EGF or NRG1 β as representative ligands for EGFR and ErbB4 signaling, respectively. However, since most human cancers express EGFR ligands other than EGF, differences in intrinsic ligand activities have not been captured by these studies and future work designed to elucidate the roles played by specific ErbB ligands in tumorigenesis and tumor progression should be expanded across the ligand family. Moreover, future studies should account for the possibility that ErbB receptor coupling to biological responses in a given tissue or cell may reflect competition between multiple EGF family ligands with different intrinsic activities. For example, increased EGFR coupling to tumor cell motility and invasiveness may be due to an increase in AR concentration. However, because EGF fails to stimulate EGFR coupling to tumor cell motility and invasiveness in some model systems (Willmarth & Ethier, 2006), a decrease in EGF concentration (and consequently, reduced competitive inhibition of AR-induced EGFR signaling) may result in increased EGFR coupling to tumor cell motility and invasiveness. Likewise, in some model systems it appears that NRG2β stimulates ErbB4 coupling to cell proliferation, whereas NRG2α fails to. Consequently, the latter ligand could function as a competitive antagonist of mitogenesis triggered by NRG2β (Wilson et al., 2007). Thus, increased ErbB4 coupling to cell proliferation may be due to an increase in NRG2β concentration or a decrease in NRG2α concentration.

4.2. EGF family ligand point mutants that display reduced intrinsic activity suggest a new paradigm for therapeutic ErbB receptor antagonists

The high degree of amino acid sequence identity between NRG2α and NRG2β suggests that a relatively small number of amino acid residues are responsible for the differences in their intrinsic activities. This hypothesis has been tested by exchanging individual amino acid residues between NRG2α and NRG2β. The NRG2β Q43L mutant, like wild-type NRG2β, potently stimulates ErbB4 tyrosine phosphorylation and appears to retain high affinity binding to ErbB4. However, unlike wild-type NRG2β, NRG2β/Q43L fails to stimulate ErbB4 coupling to cell proliferation (Wilson et al., 2007). Similarly, a variant of NRG2α with the converse L43Q mutation does not exhibit greater affinity for ErbB4, but stimulates ErbB4 coupling to cell proliferation (Wilson et al., 2007). Thus, the intrinsic activity of these EGF family members can be regulated independently of their affinity for their cognate receptor(s) and amino acid residue 43 of NRG2α and NRG2β appears to be a crucial determinant of differences in their intrinsic activities.

The observation that NRG2β/Q43L potently stimulates ErbB4 phosphorylation (Hobbs et al., 2004; Wilson et al., 2007) yet fails to stimulate ErbB4 coupling to cell proliferation (Wilson et al., 2007) makes it plausible to hypothesize that NRG2β/Q43L will competitively antagonize ligand-induced ErbB4 coupling to cell proliferation. If so, a new paradigm for receptor tyrosine kinase antagonists is suggested in which variants of other receptor tyrosine kinases peptide agonists with reduced intrinsic activity but no change in receptor binding affinity might be developed. These would function as therapeutic competitive antagonists of malignant phenotypes induced by endogenous growth factors. Therapeutic effect could be optimized with novel, functionally selective ligands such that only subsets of effectors from a single receptor are activated or inhibited, thereby increasing the therapeutic effect and minimizing adverse effects.

Moreover, our hypothesis that NRG2β/Q43L antagonizes ErbB4 signaling by stabilizing a receptor conformation that cannot couple to certain effectors suggests that it might be possible to develop small molecules that allosterically antagonize ErbB receptor signaling by stabilizing the receptor in an analogous, inactive conformation. We predict that such molecules would be resistant to the effects of ErbB receptor kinase domain mutations that disrupt the activity of small molecule ATP-competitive kinase inhibitors. Accordingly, such molecules would represent a class of receptor antagonists that are uniquely effective against certain tumors that are resistant to ErbB receptor kinase inhibitors. Clearly, further detailed mechanistic understanding of ErbB receptor activation - with a focus on understanding the effects of different ligands - will be required to take full advantage of this possibility, and could lead to a new generation of ErbB-targeted therapeutics.

Acknowledgments

The authors gratefully acknowledge the support of the National Institutes of Health (AR045585, DK067875, CA080770 and CA114209), the United States Army Medical Research and Materiel Command Breast Cancer Research Program (D AMD-17-00-1-0416), and Susan G. Komen for the Cure.

Abbreviations

AR

Amphiregulin

BTC

Betacellulin

EGF

Epidermal Growth Factor

EGFR

Epidermal Growth Factor Receptor

EPG

Epigen

EPR

Epiregulin

GPCR

G-protein-coupled receptor

HB-EGF

Heparin-binding EGF-like growth factor

MDCK

Madin-Darby canine kidney

NRG

Neuregulin

NSCLC

Non-small-cell lung carcinoma

PLCγ

Phospholipase C gamma

PTB

Phosphotyrosine-binding

SH2

Src-homology domain type 2

TGFα

Transforming Growth Factor Alpha

Footnotes

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