Abstract
Heparinase (heparin lyase, EC 4.2.2.7) was isolated from the cell extract of an oral bacterium, Bacteroides heparinolyticus. It was a basic protein with an isoelectric point of 9.5. Its molecular weight was 63,000. The enzyme was the most active against heparin among the tested mucopolysaccharides. Catalytic properties may be similar to those of heparinase of Flavobacterium heparinum, since the enzymatic degradation products obtained by using the two enzymes were the same on the basis of paper chromatography.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dzink J. L., Tanner A. C., Haffajee A. D., Socransky S. S. Gram negative species associated with active destructive periodontal lesions. J Clin Periodontol. 1985 Sep;12(8):648–659. doi: 10.1111/j.1600-051x.1985.tb00936.x. [DOI] [PubMed] [Google Scholar]
- Fujimura S., Nakamura T. Isolation and characterization of a protease from Bacteroides gingivalis. Infect Immun. 1987 Mar;55(3):716–720. doi: 10.1128/iai.55.3.716-720.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Linker A., Hovingh P. Isolation and characterization of oligosaccharides obtained from heparin by the action of heparinase. Biochemistry. 1972 Feb 15;11(4):563–568. doi: 10.1021/bi00754a013. [DOI] [PubMed] [Google Scholar]
- Loesche W. J., Syed S. A., Schmidt E., Morrison E. C. Bacterial profiles of subgingival plaques in periodontitis. J Periodontol. 1985 Aug;56(8):447–456. doi: 10.1902/jop.1985.56.8.447. [DOI] [PubMed] [Google Scholar]
- Moore W. E. Microbiology of periodontal disease. J Periodontal Res. 1987 Sep;22(5):335–341. doi: 10.1111/j.1600-0765.1987.tb01595.x. [DOI] [PubMed] [Google Scholar]
- Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
- Nakamura T., Suginaka Y., Takazoe I. Heparinase activity in lesion of periodontal diseases. Bull Tokyo Dent Coll. 1976 Aug;17(3):147–155. [PubMed] [Google Scholar]
- Takazoe I., Nakamura T. Experimental mixed infection by human gingival crevice material. Bull Tokyo Dent Coll. 1971 May;12(2):85–93. [PubMed] [Google Scholar]
- Taniguchi H., Fujimura S., Takeuchi K., Nakamura T. Purification and characterization of mucopolysaccharidase from an oral strain of Bacteroides sp. Appl Environ Microbiol. 1983 Dec;46(6):1252–1257. doi: 10.1128/aem.46.6.1252-1257.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]