Abstract
A chromatofocusing procedure for the purification of staphylococcal enterotoxin D was developed. The purification included the removal of the toxic protein from culture supernatant fluids of Staphylococcus aureus 1151m by batch adsorption with CG-50 resin, chromatofocusing on Polybuffer Exchanger 94, and gel permeation chromatography on Sephacryl S-200. The purity of the staphylococcal enterotoxin D obtained was approximately 98%.
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Selected References
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- Chang H. C., Bergdoll M. S. Purification and some physicochemical properties of staphylococcal enterotoxin D. Biochemistry. 1979 May 15;18(10):1937–1942. doi: 10.1021/bi00577a013. [DOI] [PubMed] [Google Scholar]
- Ende I. A., Terplan G., Kickhöfen B., Hammer D. K. Chromatofocusing: a new method for purification of staphylococcal enterotoxins B and C1. Appl Environ Microbiol. 1983 Dec;46(6):1323–1330. doi: 10.1128/aem.46.6.1323-1330.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fey H., Pfister H., Müller C. Simple production of staphylococcal enterotoxins (SET) with chromatofocusing and isoelectrofocusing in flat bed gels. Zentralbl Veterinarmed B. 1984 Aug;31(7):508–517. [PubMed] [Google Scholar]
- Kato E., Khan M., Kujovich L., Bergdoll M. S. Production of enterotoxin a. Appl Microbiol. 1966 Nov;14(6):966–972. doi: 10.1128/am.14.6.966-972.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Reiser R. F., Robbins R. N., Khoe G. P., Bergdoll M. S. Purification and some physicochemical properties of toxic-shock toxin. Biochemistry. 1983 Aug 2;22(16):3907–3912. doi: 10.1021/bi00285a028. [DOI] [PubMed] [Google Scholar]
- Reiser R. F., Robbins R. N., Noleto A. L., Khoe G. P., Bergdoll M. S. Identification, purification, and some physicochemical properties of staphylococcal enterotoxin C3. Infect Immun. 1984 Sep;45(3):625–630. doi: 10.1128/iai.45.3.625-630.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Robbins R., Gould S., Bergdoll M. Detecting the enterotoxigenicity of Staphylococcus aureus strains. Appl Microbiol. 1974 Dec;28(6):946–950. doi: 10.1128/am.28.6.946-950.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]