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. 1988 Jul;26(7):1416–1418. doi: 10.1128/jcm.26.7.1416-1418.1988

Rapid and sensitive viral culture method for human immunodeficiency virus type 1.

J B Jackson 1, R W Coombs 1, K Sannerud 1, F S Rhame 1, H H Balfour Jr 1
PMCID: PMC266626  PMID: 3165981

Abstract

Peripheral blood mononuclear cells from 142 consecutive patients with antibodies to human immunodeficiency virus type 1 (HIV-1) were cultured for HIV-1. All 72 patients with symptoms of HIV-1 infection were culture positive, as were 69 of 70 asymptomatic patients. Of the 142 patients, 132 (93%) were culture positive within 10 days after initiation of the culture.

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Selected References

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  1. Bolton V., Pedersen N. C., Higgins J., Jennings M., Carlson J. Unique p24 epitope marker to identify multiple human immunodeficiency virus variants in blood from the same individuals. J Clin Microbiol. 1987 Aug;25(8):1411–1415. doi: 10.1128/jcm.25.8.1411-1415.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Feorino P., Forrester B., Schable C., Warfield D., Schochetman G. Comparison of antigen assay and reverse transcriptase assay for detecting human immunodeficiency virus in culture. J Clin Microbiol. 1987 Dec;25(12):2344–2346. doi: 10.1128/jcm.25.12.2344-2346.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gallo D., Kimpton J. S., Dailey P. J. Comparative studies on use of fresh and frozen peripheral blood lymphocyte specimens for isolation of human immunodeficiency virus and effects of cell lysis on isolation efficiency. J Clin Microbiol. 1987 Jul;25(7):1291–1294. doi: 10.1128/jcm.25.7.1291-1294.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goudsmit J., Lange J. M., Paul D. A., Dawson G. J. Antigenemia and antibody titers to core and envelope antigens in AIDS, AIDS-related complex, and subclinical human immunodeficiency virus infection. J Infect Dis. 1987 Mar;155(3):558–560. doi: 10.1093/infdis/155.3.558. [DOI] [PubMed] [Google Scholar]
  5. Jackson B., Sannerud K., Rhame F., Tsang R., Balfour H. H., Jr Comparison of reverse transcriptase assay with the Retro-Tek viral capture assay for detection of human immunodeficiency virus. Diagn Microbiol Infect Dis. 1987 Jul;7(3):185–192. doi: 10.1016/0732-8893(87)90003-4. [DOI] [PubMed] [Google Scholar]
  6. Levy J. A., Shimabukuro J. Recovery of AIDS-associated retroviruses from patients with AIDS or AIDS-related conditions and from clinically healthy individuals. J Infect Dis. 1985 Oct;152(4):734–738. doi: 10.1093/infdis/152.4.734. [DOI] [PubMed] [Google Scholar]
  7. Newell A. L., Malkovsky M., Orr D., Taylor-Robinson D., Dalgleish A. G. Antigen test versus reverse transcriptase assay for detecting HIV. Lancet. 1987 Nov 14;2(8568):1146–1147. doi: 10.1016/s0140-6736(87)91570-4. [DOI] [PubMed] [Google Scholar]
  8. Paul D. A., Falk L. A., Kessler H. A., Chase R. M., Blaauw B., Chudwin D. S., Landay A. L. Correlation of serum HIV antigen and antibody with clinical status in HIV-infected patients. J Med Virol. 1987 Aug;22(4):357–363. doi: 10.1002/jmv.1890220408. [DOI] [PubMed] [Google Scholar]
  9. Ranki A., Valle S. L., Krohn M., Antonen J., Allain J. P., Leuther M., Franchini G., Krohn K. Long latency precedes overt seroconversion in sexually transmitted human-immunodeficiency-virus infection. Lancet. 1987 Sep 12;2(8559):589–593. doi: 10.1016/s0140-6736(87)92985-0. [DOI] [PubMed] [Google Scholar]
  10. Schaffner C. P., Plescia O. J., Pontani D., Sun D., Thornton A., Pandey R. C., Sarin P. S. Anti-viral activity of amphotericin B methyl ester: inhibition of HTLV-III replication in cell culture. Biochem Pharmacol. 1986 Nov 15;35(22):4110–4113. doi: 10.1016/0006-2952(86)90037-7. [DOI] [PubMed] [Google Scholar]
  11. Ward J. W., Grindon A. J., Feorino P. M., Schable C., Parvin M., Allen J. R. Laboratory and epidemiologic evaluation of an enzyme immunoassay for antibodies to HTLV-III. JAMA. 1986 Jul 18;256(3):357–361. [PubMed] [Google Scholar]

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