Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 Jul;26(7):1421–1424. doi: 10.1128/jcm.26.7.1421-1424.1988

Antibody response to Campylobacter coli in children during intestinal infection and carriage.

P M Martin 1, J Mathiot 1, J Ipero 1, A J Georges 1, M C Georges-Courbot 1
PMCID: PMC266628  PMID: 3410956

Abstract

In the Central African Republic, etiological studies of diarrhea have shown that Campylobacter coli accounts for almost 40% of Campylobacter enteric isolations. This prompted us to investigate the antibody response to C. coli infection in children. As expected from the literature on Campylobacter jejuni infections, our results show that both infection and carriage elicited antibodies against glycine-extracted membrane antigens, flagella, and cholera toxin. The human antibody response to C. coli resembles the response to C. jejuni, and this similarity will allow comparative studies on larger numbers of infections, both symptomatic and asymptomatic. Anti-cholera toxin antibodies were directed against both the A and B subunits.

Full text

PDF
1421

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atanasiu P., Perrin P., Delagneau J. F. Use of an enzyme immunoassay with protein A for rabies antigen and antibody determination. Dev Biol Stand. 1980;46:207–215. [PubMed] [Google Scholar]
  2. Burnette W. N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. doi: 10.1016/0003-2697(81)90281-5. [DOI] [PubMed] [Google Scholar]
  3. Butzler J. P., Skirrow M. B. Campylobacter enteritis. Clin Gastroenterol. 1979 Sep;8(3):737–765. [PubMed] [Google Scholar]
  4. Georges-Courbot M. C., Baya C., Beraud A. M., Meunier D. M., Georges A. J. Distribution and serotypes of Campylobacter jejuni and Campylobacter coli in enteric Campylobacter strains isolated from children in the Central African Republic. J Clin Microbiol. 1986 Mar;23(3):592–594. doi: 10.1128/jcm.23.3.592-594.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Georges-Courbot M. C., Beraud-Cassel A. M., Gouandjika I., Georges A. J. Prospective study of enteric Campylobacter infections in children from birth to 6 months in the Central African Republic. J Clin Microbiol. 1987 May;25(5):836–839. doi: 10.1128/jcm.25.5.836-839.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Georges M. C., Wachsmuth I. K., Meunier D. M., Nebout N., Didier F., Siopathis M. R., Georges A. J. Parasitic, bacterial, and viral enteric pathogens associated with diarrhea in the Central African Republic. J Clin Microbiol. 1984 May;19(5):571–575. doi: 10.1128/jcm.19.5.571-575.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Harvey S. M. Hippurate hydrolysis by Campylobacter fetus. J Clin Microbiol. 1980 Apr;11(4):435–437. doi: 10.1128/jcm.11.4.435-437.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Karmali M. A., Penner J. L., Fleming P. C., Williams A., Hennessy J. N. The serotype and biotype distribution of clinical isolates of Campylobacter jejuni and Campylobacter coli over a three-year period. J Infect Dis. 1983 Feb;147(2):243–246. doi: 10.1093/infdis/147.2.243. [DOI] [PubMed] [Google Scholar]
  9. Klipstein F. A., Engert R. F. Immunological relationship of the B subunits of Campylobacter jejuni and Escherichia coli heat-labile enterotoxins. Infect Immun. 1985 Jun;48(3):629–633. doi: 10.1128/iai.48.3.629-633.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Lassen J., Kapperud G. Epidemiological aspects of enteritis due to Campylobacter spp. in Norway. J Clin Microbiol. 1984 Feb;19(2):153–156. doi: 10.1128/jcm.19.2.153-156.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Logan S. M., Trust T. J. Molecular identification of surface protein antigens of Campylobacter jejuni. Infect Immun. 1983 Nov;42(2):675–682. doi: 10.1128/iai.42.2.675-682.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McSweegan E., Walker R. I. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun. 1986 Jul;53(1):141–148. doi: 10.1128/iai.53.1.141-148.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mills S. D., Bradbury W. C. Human antibody response to outer membrane proteins of Campylobacter jejuni during infection. Infect Immun. 1984 Feb;43(2):739–743. doi: 10.1128/iai.43.2.739-743.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mills S. D., Bradbury W. C., Penner J. L. Isolation and characterization of a common antigen in Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 1986 Jul;24(1):69–75. doi: 10.1128/jcm.24.1.69-75.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morooka T., Umeda A., Amako K. Motility as an intestinal colonization factor for Campylobacter jejuni. J Gen Microbiol. 1985 Aug;131(8):1973–1980. doi: 10.1099/00221287-131-8-1973. [DOI] [PubMed] [Google Scholar]
  18. Nachamkin I., Hart A. M. Western blot analysis of the human antibody response to Campylobacter jejuni cellular antigens during gastrointestinal infection. J Clin Microbiol. 1985 Jan;21(1):33–38. doi: 10.1128/jcm.21.1.33-38.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Newell D. G., McBride H., Pearson A. D. The identification of outer membrane proteins and flagella of Campylobacter jejuni. J Gen Microbiol. 1984 May;130(5):1201–1208. doi: 10.1099/00221287-130-5-1201. [DOI] [PubMed] [Google Scholar]
  20. Olsvik O., Wachsmuth K., Morris G., Feeley J. C. Genetic probing of Campylobacter jejuni for cholera toxin and Escherichia coli heat-labile enterotoxin. Lancet. 1984 Feb 25;1(8374):449–449. doi: 10.1016/s0140-6736(84)91773-2. [DOI] [PubMed] [Google Scholar]
  21. Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Roop R. M., 2nd, Smibert R. M., Johnson J. L., Krieg N. R. Differential characteristics of catalase-positive campylobacters correlated with DNA homology groups. Can J Microbiol. 1984 Jul;30(7):938–951. doi: 10.1139/m84-147. [DOI] [PubMed] [Google Scholar]
  23. Ruiz-Palacios G. M., Torres J., Torres N. I., Escamilla E., Ruiz-Palacios B. R., Tamayo J. Cholera-like enterotoxin produced by Campylobacter jejuni. Characterisation and clinical significance. Lancet. 1983 Jul 30;2(8344):250–253. doi: 10.1016/s0140-6736(83)90234-9. [DOI] [PubMed] [Google Scholar]
  24. Skirrow M. B., Benjamin J. Differentiation of enteropathogenic Campylobacter. J Clin Pathol. 1980 Nov;33(11):1122–1122. doi: 10.1136/jcp.33.11.1122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wray W., Boulikas T., Wray V. P., Hancock R. Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981 Nov 15;118(1):197–203. doi: 10.1016/0003-2697(81)90179-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES