Abstract
A method that uses a 48-well tissue culture cluster tray system for the isolation of Chlamydia trachomatis is described. The cluster tray system was as sensitive (100%) as and more time efficient than the conventional cover slip method, thereby being considerably cost saving. With both culture methods, the prevalence rates of genital carriage of C. trachomatis in women attending clinics for legal abortion and for cervical dysplasia were 5% (31 of 641 patients) and 2% (3 of 148 patients), respectively.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alexander E. R., Harrison H. R. Role of Chlamydia trachomatis in perinatal infection. Rev Infect Dis. 1983 Jul-Aug;5(4):713–719. doi: 10.1093/clinids/5.4.713. [DOI] [PubMed] [Google Scholar]
- BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coudron P. E., Fedorko D. P., Dawson M. S., Kaplowitz L. G., Brookman R. R., Dalton H. P., Davis B. A. Detection of Chlamydia trachomatis in genital specimens by the Microtrak direct specimen test. Am J Clin Pathol. 1986 Jan;85(1):89–92. doi: 10.1093/ajcp/85.1.89. [DOI] [PubMed] [Google Scholar]
- Lipkin E. S., Moncada J. V., Shafer M. A., Wilson T. E., Schachter J. Comparison of monoclonal antibody staining and culture in diagnosing cervical chlamydial infection. J Clin Microbiol. 1986 Jan;23(1):114–117. doi: 10.1128/jcm.23.1.114-117.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mallinson H., Sikotra S., Arya O. P. Cultural method for large-scale screening for Chlamydia trachomatis genital infection. J Clin Pathol. 1981 Jul;34(7):712–718. doi: 10.1136/jcp.34.7.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mårdh P. A., Helin I., Bobeck S., Laurin J., Nilsson T. Colonisation of pregnant and puerperal women and neonates with Chlamydia trachomatis. Br J Vener Dis. 1980 Apr;56(2):96–100. doi: 10.1136/sti.56.2.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Møller B. R., Ahrons S., Laurin J., Mårdh P. A. Pelvic infection after elective abortion associated with Chlamydia trachomatis. Obstet Gynecol. 1982 Feb;59(2):210–213. [PubMed] [Google Scholar]
- Oriel J. D., Johnson A. L., Barlow D., Thomas B. J., Nayyar K., Reeve P. Infection of the uterine cervix with Chlamydia trachomatis. J Infect Dis. 1978 Apr;137(4):443–451. doi: 10.1093/infdis/137.4.443. [DOI] [PubMed] [Google Scholar]
- Paavonen J., Vesterinen E., Meyer B., Saikku P., Suni J., Purola E., Saksela E. Genital Chlamydia trachomatis infections in patients with cervical atypia. Obstet Gynecol. 1979 Sep;54(3):289–291. [PubMed] [Google Scholar]
- Pothier P., Kazmierczak A. Comparison of cell culture with two direct Chlamydia tests using immunofluorescence or enzyme-linked immunosorbent assay. Eur J Clin Microbiol. 1986 Oct;5(5):569–572. doi: 10.1007/BF02017707. [DOI] [PubMed] [Google Scholar]
- Schachter J. Chlamydial infections (second of three parts). N Engl J Med. 1978 Mar 2;298(9):490–495. doi: 10.1056/NEJM197803022980905. [DOI] [PubMed] [Google Scholar]
- Schachter J., Hill E. C., King E. B., Coleman V. R., Jones P., Meyer K. F. Chlamydial infection in women with cervical dysplasia. Am J Obstet Gynecol. 1975 Dec 1;123(7):753–757. doi: 10.1016/0002-9378(75)90501-3. [DOI] [PubMed] [Google Scholar]
- Smith J. W., Rogers R. E., Katz B. P., Brickler J. F., Lineback P. L., Van der Pol B., Jones R. B. Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. J Clin Microbiol. 1987 May;25(5):868–872. doi: 10.1128/jcm.25.5.868-872.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
