Abstract
BACTEC 13A medium (Johnston Laboratories, Towson, Md.) was compared with Isolator (Du Pont Co., Wilmington, Del.) concentrate for sensitivity, speed, and technical ease of isolation of mycobacteria from paired patient blood samples. Of 72 positive cultures, 63 were positive by both systems. Five positive cultures were detected by BACTEC 13A medium alone, and four were detected by Isolator alone. The median numbers of days to positivity were 12 for BACTEC 13A medium and 14 for Isolator concentrate. BACTEC 13A medium has an advantage over the Isolator in requiring less laboratory manipulation of the specimen but has the disadvantages of not providing isolated colonies or quantitation of organisms. Some technical problems with contamination in both systems are also discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gill V. J., Park C. H., Stock F., Gosey L. L., Witebsky F. G., Masur H. Use of lysis-centrifugation (isolator) and radiometric (BACTEC) blood culture systems for the detection of mycobacteremia. J Clin Microbiol. 1985 Oct;22(4):543–546. doi: 10.1128/jcm.22.4.543-546.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kiehn T. E., Cammarata R. Laboratory diagnosis of mycobacterial infections in patients with acquired immunodeficiency syndrome. J Clin Microbiol. 1986 Nov;24(5):708–711. doi: 10.1128/jcm.24.5.708-711.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lévy-Frébault V., Pangon B., Buré A., Katlama C., Marche C., David H. L. Mycobacterium simiae and Mycobacterium avium-M. intracellulare mixed infection in acquired immune deficiency syndrome. J Clin Microbiol. 1987 Jan;25(1):154–157. doi: 10.1128/jcm.25.1.154-157.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macher A. M., Kovacs J. A., Gill V., Roberts G. D., Ames J., Park C. H., Straus S., Lane H. C., Parrillo J. E., Fauci A. S. Bacteremia due to Mycobacterium avium-intracellulare in the acquired immunodeficiency syndrome. Ann Intern Med. 1983 Dec;99(6):782–785. doi: 10.7326/0003-4819-99-6-782. [DOI] [PubMed] [Google Scholar]
- Masur H., Tuazon C., Gill V., Grimes G., Baird B., Fauci A. S., Lane H. C. Effect of combined clofazimine and ansamycin therapy on Mycobacterium avium-Mycobacterium intracellulare bacteremia in patients with AIDS. J Infect Dis. 1987 Jan;155(1):127–129. doi: 10.1093/infdis/155.1.127. [DOI] [PubMed] [Google Scholar]
- Pierce P. F., DeYoung D. R., Roberts G. D. Mycobacteremia and the new blood culture systems. Ann Intern Med. 1983 Dec;99(6):786–789. doi: 10.7326/0003-4819-99-6-786. [DOI] [PubMed] [Google Scholar]
- Rogers P. L., Walker R. E., Lane H. C., Witebsky F. G., Kovacs J. A., Parrillo J. E., Masur H. Disseminated Mycobacterium haemophilum infection in two patients with the acquired immunodeficiency syndrome. Am J Med. 1988 Mar;84(3 Pt 2):640–642. doi: 10.1016/0002-9343(88)90150-7. [DOI] [PubMed] [Google Scholar]
- Young L. S., Inderlied C. B., Berlin O. G., Gottlieb M. S. Mycobacterial infections in AIDS patients, with an emphasis on the Mycobacterium avium complex. Rev Infect Dis. 1986 Nov-Dec;8(6):1024–1033. doi: 10.1093/clinids/8.6.1024. [DOI] [PubMed] [Google Scholar]