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. 1988 Aug;26(8):1555–1560. doi: 10.1128/jcm.26.8.1555-1560.1988

Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II.

C Handl 1, B Rönnberg 1, B Nilsson 1, E Olsson 1, H Jonsson 1, J I Flock 1
PMCID: PMC266659  PMID: 3049659

Abstract

The gene for Escherichia coli heat-stable enterotoxin type II (STII) was fused to the genes for protein A from Staphylococcus aureus and beta-galactosidase in two different expression systems. Antibodies raised in rabbits against the protein A-STII fusion protein recognized the beta-galactosidase-STII fusion protein. The latter fusion protein was used as the immobilized antigen in an enzyme-linked immunosorbent assay (ELISA) for detection of STII. The correlation between the results of the ELISA and the intestinal loop test in piglets was 95%, suggesting that the ELISA can be used to reliably detect STII.

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Selected References

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