Abstract
To facilitate the diagnosis of Pneumocystis carinii from bronchoalveolar lavage and sputum specimens, we have defined conditions for optimal use of the cytocentrifuge for this purpose. Centrifugation in the cytocentrifuge at 1,200 rpm for 10 min yielded the best recovery of P. carinii. To reliably ensure complete absorption of the fluid specimen from the cytocentrifuge chamber, it was necessary to use two absorption filters simultaneously. Different methods of treating induced sputum with mucolytic agents to process sputum with the cytocentrifuge were tried. Results of these studies and our current method for treating sputa are discussed. Comparisons of slides prepared by traditional centrifugation and by cytocentrifuge processing showed the latter to be equally effective for detecting P. carinii. The most prominent advantage of the cytocentrifuge was the much smaller area to review and consequently the shortened time required to read the slides.
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Selected References
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- Bigby T. D., Margolskee D., Curtis J. L., Michael P. F., Sheppard D., Hadley W. K., Hopewell P. C. The usefulness of induced sputum in the diagnosis of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome. Am Rev Respir Dis. 1986 Apr;133(4):515–518. doi: 10.1164/arrd.1986.133.4.515. [DOI] [PubMed] [Google Scholar]
- Gill V. J., Evans G., Stock F., Parrillo J. E., Masur H., Kovacs J. A. Detection of Pneumocystis carinii by fluorescent-antibody stain using a combination of three monoclonal antibodies. J Clin Microbiol. 1987 Oct;25(10):1837–1840. doi: 10.1128/jcm.25.10.1837-1840.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gosey L. L., Howard R. M., Witebsky F. G., Ognibene F. P., Wu T. C., Gill V. J., MacLowry J. D. Advantages of a modified toluidine blue O stain and bronchoalveolar lavage for the diagnosis of Pneumocystis carinii pneumonia. J Clin Microbiol. 1985 Nov;22(5):803–807. doi: 10.1128/jcm.22.5.803-807.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin W. J., 2nd, Smith T. F. Rapid detection of cytomegalovirus in bronchoalveolar lavage specimens by a monoclonal antibody method. J Clin Microbiol. 1986 Jun;23(6):1006–1008. doi: 10.1128/jcm.23.6.1006-1008.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ognibene F. P., Shelhamer J., Gill V., Macher A. M., Loew D., Parker M. M., Gelmann E., Fauci A. S., Parrillo J. E., Masur H. The diagnosis of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome using subsegmental bronchoalveolar lavage. Am Rev Respir Dis. 1984 Jun;129(6):929–932. doi: 10.1164/arrd.1984.129.6.929. [DOI] [PubMed] [Google Scholar]
- Pitchenik A. E., Ganjei P., Torres A., Evans D. A., Rubin E., Baier H. Sputum examination for the diagnosis of Pneumocystis carinii pneumonia in the acquired immunodeficiency syndrome. Am Rev Respir Dis. 1986 Feb;133(2):226–229. doi: 10.1164/arrd.1986.133.2.226. [DOI] [PubMed] [Google Scholar]
- Shanholtzer C. J., Schaper P. J., Peterson L. R. Concentrated gram stain smears prepared with a cytospin centrifuge. J Clin Microbiol. 1982 Dec;16(6):1052–1056. doi: 10.1128/jcm.16.6.1052-1056.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]