Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 Sep;26(9):1720–1724. doi: 10.1128/jcm.26.9.1720-1724.1988

Candida albicans- and Candida stellatoidea-specific DNA fragment.

J E Cutler 1, P M Glee 1, H L Horn 1
PMCID: PMC266703  PMID: 2460494

Abstract

DNA was isolated from whole cells of Candida albicans and digested with MspI restriction enzyme. In addition to the expected large number of low-molecular-weight DNA pieces resulting from the digestion, multiple high-molecular-weight (greater than 3.0 kilobase pairs) fragments were generated by this enzyme, which cleaves DNA at CCGG sequences. Some of these fragments appeared highly repeated. An MspI fragment which was similar in size to one of the repeat elements (2.9 kilobase pairs) was cloned into the ClaI site of the plasmid vector pBR322 and replicated in a suitable Escherichia coli host strain. The candidal fragment was radiolabeled and used to probe Southern blots of DNA from several Candida species, various other fungi, and mouse and human cells. Only DNA from C. albicans and a strain of Candida stellatoidea was found to contain sequences of significant homology for hybridization. The cloned fragment may possibly be of use as a DNA probe for detection of the presence of C. albicans.

Full text

PDF
1720

Images in this article

1722Image
on p.1722
1722Image
on p.1722
1723Image
on p.1723

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  2. Glee P. M., Russell P. J., Welsch J. A., Pratt J. C., Cutler J. E. Methods for DNA extraction from Candida albicans. Anal Biochem. 1987 Jul;164(1):207–213. doi: 10.1016/0003-2697(87)90387-3. [DOI] [PubMed] [Google Scholar]
  3. Mason M. M., Lasker B. A., Riggsby W. S. Molecular probe for identification of medically important Candida species and Torulopsis glabrata. J Clin Microbiol. 1987 Mar;25(3):563–566. doi: 10.1128/jcm.25.3.563-566.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Norval M., Bingham R. W. Advances in the use of nucleic acid probes in diagnosis of viral diseases of man. Brief review. Arch Virol. 1987;97(3-4):151–165. doi: 10.1007/BF01314418. [DOI] [PubMed] [Google Scholar]
  5. Peden K. W. Revised sequence of the tetracycline-resistance gene of pBR322. Gene. 1983 May-Jun;22(2-3):277–280. doi: 10.1016/0378-1119(83)90112-9. [DOI] [PubMed] [Google Scholar]
  6. Resnick S., Pappagianis D., McKerrow J. H. Proteinase production by the parasitic cycle of the pathogenic fungus Coccidioides immitis. Infect Immun. 1987 Nov;55(11):2807–2815. doi: 10.1128/iai.55.11.2807-2815.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  8. Rodland K. D., Russell P. J. Regulation of ribosomal RNA cistron number in a strain of Neurospora crassa with a duplication of the nucleolus organizer region. Biochim Biophys Acta. 1982 May 31;697(2):162–169. doi: 10.1016/0167-4781(82)90072-0. [DOI] [PubMed] [Google Scholar]
  9. Russell P. J., Welsch J. A., Rachlin E. M., McCloskey J. A. Different levels of DNA methylation in yeast and mycelial forms of Candida albicans. J Bacteriol. 1987 Sep;169(9):4393–4395. doi: 10.1128/jb.169.9.4393-4395.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SKOOG W. A., BECK W. S. Studies on the fibrinogen, dextran and phytohemagglutinin methods of isolating leukocytes. Blood. 1956 May;11(5):436–454. [PubMed] [Google Scholar]
  11. Schaffer H. E., Sederoff R. R. Improved estimation of DNA fragment lengths from Agarose gels. Anal Biochem. 1981 Jul 15;115(1):113–122. doi: 10.1016/0003-2697(81)90533-9. [DOI] [PubMed] [Google Scholar]
  12. Scherer S., Stevens D. A. A Candida albicans dispersed, repeated gene family and its epidemiologic applications. Proc Natl Acad Sci U S A. 1988 Mar;85(5):1452–1456. doi: 10.1073/pnas.85.5.1452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scherer S., Stevens D. A. Application of DNA typing methods to epidemiology and taxonomy of Candida species. J Clin Microbiol. 1987 Apr;25(4):675–679. doi: 10.1128/jcm.25.4.675-679.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Soll D. R., Langtimm C. J., McDowell J., Hicks J., Galask R. High-frequency switching in Candida strains isolated from vaginitis patients. J Clin Microbiol. 1987 Sep;25(9):1611–1622. doi: 10.1128/jcm.25.9.1611-1622.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  16. Storck R., Alexopoulos C. J. Deoxyribonucleic acid of fungi. Bacteriol Rev. 1970 Jun;34(2):126–154. doi: 10.1128/br.34.2.126-154.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  18. Vincent R. D., Goewert R., Goldman W. E., Kobayashi G. S., Lambowitz A. M., Medoff G. Classification of Histoplasma capsulatum isolates by restriction fragment polymorphisms. J Bacteriol. 1986 Mar;165(3):813–818. doi: 10.1128/jb.165.3.813-818.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wills J. W., Troutman W. B., Riggsby W. S. Circular mitochondrial genome of Candida albicans contains a large inverted duplication. J Bacteriol. 1985 Oct;164(1):7–13. doi: 10.1128/jb.164.1.7-13.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES