Abstract
Using purified elementary bodies of 14 Chlamydia trachomatis serovars in an in vitro assay, we compared the staining characteristics of six commercially available monoclonal antibody reagents used for direct immunofluorescent staining of patient smears. Considerable variation in the degree of brightness, consistency of staining, and specificity of the six reagents was found. Monoclonal antibodies against the major outer membrane proteins of C. trachomatis produced brighter fluorescence, more consistent elementary body morphology, and less nonspecific staining than did monoclonal antibodies directed against chlamydial lipopolysaccharide.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alexander I., Paul I. D., Caul E. O. Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence. Genitourin Med. 1985 Aug;61(4):252–254. doi: 10.1136/sti.61.4.252. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Forbes B. A., Bartholoma N., McMillan J., Roefaro M., Weiner L., Welych L. Evaluation of a monoclonal antibody test to detect chlamydia in cervical and urethral specimens. J Clin Microbiol. 1986 Jun;23(6):1136–1137. doi: 10.1128/jcm.23.6.1136-1137.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Munday P. E., Thomas B. J., Taylor-Robinson D. The Micro Trak test for rapid detection of chlamydiae in diagnosing and managing women with abdominal pain. Genitourin Med. 1986 Feb;62(1):15–16. doi: 10.1136/sti.62.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saikku P., Puolakkainen M., Leinonen M., Nurminen M., Nissinen A. Cross-reactivity between Chlamydiazyme and Acinetobacter strains. N Engl J Med. 1986 Apr 3;314(14):922–923. doi: 10.1056/NEJM198604033141413. [DOI] [PubMed] [Google Scholar]
- Stamm W. E. Diagnosis of Chlamydia trachomatis genitourinary infections. Ann Intern Med. 1988 May;108(5):710–717. doi: 10.7326/0003-4819-108-5-710. [DOI] [PubMed] [Google Scholar]
- Stamm W. E., Harrison H. R., Alexander E. R., Cles L. D., Spence M. R., Quinn T. C. Diagnosis of Chlamydia trachomatis infections by direct immunofluorescence staining of genital secretions. A multicenter trial. Ann Intern Med. 1984 Nov;101(5):638–641. doi: 10.7326/0003-4819-101-5-638. [DOI] [PubMed] [Google Scholar]
- Stephens R. S., Tam M. R., Kuo C. C., Nowinski R. C. Monoclonal antibodies to Chlamydia trachomatis: antibody specificities and antigen characterization. J Immunol. 1982 Mar;128(3):1083–1089. [PubMed] [Google Scholar]
- Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]
- Tjiam K. H., van Eijk R. V., van Heijst B. Y., Tideman G. J., van Joost T., Stolz E., Michel M. F. Evaluation of the direct fluorescent antibody test for diagnosis of chlamydial infections. Eur J Clin Microbiol. 1985 Dec;4(6):548–552. doi: 10.1007/BF02013392. [DOI] [PubMed] [Google Scholar]
- Uyeda C. T., Welborn P., Ellison-Birang N., Shunk K., Tsaouse B. Rapid diagnosis of chlamydial infections with the MicroTrak direct test. J Clin Microbiol. 1984 Nov;20(5):948–950. doi: 10.1128/jcm.20.5.948-950.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang S. P., Kuo C. C., Grayston J. T. Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test. J Clin Microbiol. 1979 Aug;10(2):259–261. doi: 10.1128/jcm.10.2.259-261.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]