Abstract
Gas-liquid chromatography of cellular fatty acids was used in automatic identification of clinical bacterial isolates. The intraspecies variation in the occurrence of fatty acids and the variation in the relative gas-liquid chromatography peak areas of different fatty acids were evaluated and compared with the relative peak areas of these acids. A new chromatogram comparison method involving the use of an exponential function was developed to adjust to data variation optimally. This method was compared with several previously published methods of correlation analysis with data from representative clinical bacteriological isolates. The efficacies of the methods in separating different bacterial species into distinct clusters were compared. The new exponential function method was superior to the others both in its ability to separate species into different clusters and in giving a greater degree of identity to strains within a proper cluster. The results indicate that the gas-liquid chromatography of bacterial cellular fatty acids can be used effectively in the identification of clinically isolated bacteria. However, the usefulness of the analysis depends on the comparison method used and on its ability to cope with data variations.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Drucker D. B. Chemotaxonomic fatty-acid fingerprints of some streptococci with subsequent statistical analysis. Can J Microbiol. 1974 Dec;20(12):1723–1728. doi: 10.1139/m74-266. [DOI] [PubMed] [Google Scholar]
- Guerrant G. O., Lambert M. A., Moss C. W. Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography. J Clin Microbiol. 1982 Aug;16(2):355–360. doi: 10.1128/jcm.16.2.355-360.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halebian S., Harris B., Finegold S. M., Rolfe R. D. Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria. J Clin Microbiol. 1981 Mar;13(3):444–448. doi: 10.1128/jcm.13.3.444-448.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hansen M. V., Elliott L. P. New presumptive identification test for Clostridium perfringens: reverse CAMP test. J Clin Microbiol. 1980 Oct;12(4):617–619. doi: 10.1128/jcm.12.4.617-619.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jantzen E., Bergan T., Bovre K. Gas chromatography of bacterial whole cell methanolysates; VI. Fatty acid composition of strains within Micrococcaceae;. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):785–798. [PubMed] [Google Scholar]
- Jantzen E., Bryn K., Bergan T., Bovre K. Gas chromatography of bacterial whole cell methanolysates; V. Fatty acid composition of Neisseriae and Moraxellae. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):767–779. doi: 10.1111/j.1699-0463.1974.tb02374.x. [DOI] [PubMed] [Google Scholar]
- Moss C. W., Nunez-Montiel O. L. Analysis of short-chain acids from bacteria by gas-liquid chromatography with a fused-silica capillary column. J Clin Microbiol. 1982 Feb;15(2):308–311. doi: 10.1128/jcm.15.2.308-311.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rizzo A. F., Korkeala H., Mononen I. Gas chromatography analysis of cellular fatty acids and neutral monosaccharides in the identification of lactobacilli. Appl Environ Microbiol. 1987 Dec;53(12):2883–2888. doi: 10.1128/aem.53.12.2883-2888.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]