Abstract
A reproducible method is described for the determination of the MICs of ketoconazole, miconazole, fluconazole, and itraconazole with sharp endpoints when employed with either yeasts or molds. A semisolid medium is used with controlled pH and standardized inoculum. The time of reading results is a critical factor in the conduct of this test. The medium is simple to prepare and has a relatively long refrigerator shelf life in a user-ready state, requiring only the addition of a freshly prepared inoculum after restoration to room temperature.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brass C., Shainhouse J. Z., Stevens D. A. Variability of agar dilution-replicator method of yeast susceptibility testing. Antimicrob Agents Chemother. 1979 Jun;15(6):763–768. doi: 10.1128/aac.15.6.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Galgiani J. N., Reiser J., Brass C., Espinel-Ingroff A., Gordon M. A., Kerkering T. M. Comparison of relative susceptibilities of Candida species to three antifungal agents as determined by unstandardized methods. Antimicrob Agents Chemother. 1987 Sep;31(9):1343–1347. doi: 10.1128/aac.31.9.1343. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haller I. Zum Wirkungstyp von Imidazol-Antimykotika: Konsequenzen für die Empfindlichkeitsbestimmung. Mykosen. 1979 Dec;22(12):423–430. [PubMed] [Google Scholar]
- Hoeprich P. D., Merry J. M. Influence of culture medium on susceptibility testing with BAY n 7133 and ketoconazole. J Clin Microbiol. 1986 Aug;24(2):269–271. doi: 10.1128/jcm.24.2.269-271.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hughes C. E., Bennett R. L., Beggs W. H. Broth dilution testing of Candida albicans susceptibility to ketoconazole. Antimicrob Agents Chemother. 1987 Apr;31(4):643–646. doi: 10.1128/aac.31.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minagawa H., Kitaura K., Nakamizo N. Effects of pH on the activity of ketoconazole against Candida albicans. Antimicrob Agents Chemother. 1983 Jan;23(1):105–107. doi: 10.1128/aac.23.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Odds F. C., Abbott A. B., Pye G., Troke P. F. Improved method for estimation of azole antifungal inhibitory concentrations against Candida species, based on azole/antibiotic interactions. J Med Vet Mycol. 1986 Aug;24(4):305–311. doi: 10.1080/02681218680000461. [DOI] [PubMed] [Google Scholar]