Abstract
An in situ biotinylated DNA probe assay was evaluated as an adjunct to anti-cytomegalovirus early nuclear antigen indirect immunofluorescence and cytopathic effect on cytomegalovirus-infected monolayers in shell vial cultures. Viral infection was detected by early nuclear antigen indirect immunofluorescence at 24 h and by DNA probe assay and shell vial cytopathic effect at 5 days.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brigati D. J., Myerson D., Leary J. J., Spalholz B., Travis S. Z., Fong C. K., Hsiung G. D., Ward D. C. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology. 1983 Apr 15;126(1):32–50. doi: 10.1016/0042-6822(83)90460-9. [DOI] [PubMed] [Google Scholar]
- Espy M. J., Smith T. F. Detection of herpes simplex virus in conventional tube cell cultures and in shell vials with a DNA probe kit and monoclonal antibodies. J Clin Microbiol. 1988 Jan;26(1):22–24. doi: 10.1128/jcm.26.1.22-24.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fiala M., Payne J. E., Berne T. V., Moore T. C., Henle W., Montgomerie J. Z., Chatterjee S. N., Guze L. B. Epidemiology of cytomegalovirus infection after transplantation and immunosuppression. J Infect Dis. 1975 Oct;132(4):421–433. doi: 10.1093/infdis/132.4.421. [DOI] [PubMed] [Google Scholar]
- Fung J. C., Shanley J., Tilton R. C. Comparison of the detection of herpes simplex virus in direct clinical specimens with herpes simplex virus-specific DNA probes and monoclonal antibodies. J Clin Microbiol. 1985 Nov;22(5):748–753. doi: 10.1128/jcm.22.5.748-753.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gallyas F., Görcs T., Merchenthaler I. High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry. J Histochem Cytochem. 1982 Feb;30(2):183–184. doi: 10.1177/30.2.7061820. [DOI] [PubMed] [Google Scholar]
- Gleaves C. A., Smith T. F., Shuster E. A., Pearson G. R. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens. J Clin Microbiol. 1985 Feb;21(2):217–221. doi: 10.1128/jcm.21.2.217-221.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Griffiths P. D., Panjwani D. D., Stirk P. R., Ball M. G., Ganczakowski M., Blacklock H. A., Prentice H. G. Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci. Lancet. 1984 Dec 1;2(8414):1242–1245. doi: 10.1016/s0140-6736(84)92797-1. [DOI] [PubMed] [Google Scholar]
- Hilborne L. H., Nieberg R. K., Cheng L., Lewin K. J. Direct in situ hybridization for rapid detection of cytomegalovirus in bronchoalveolar lavage. Am J Clin Pathol. 1987 Jun;87(6):766–769. doi: 10.1093/ajcp/87.6.766. [DOI] [PubMed] [Google Scholar]
- Kinney J. S., Onorato I. M., Stewart J. A., Pass R. F., Stagno S., Cheeseman S. H., Chin J., Kumar M. L., Yaeger A. S., Herrmann K. L. Cytomegaloviral infection and disease. J Infect Dis. 1985 May;151(5):772–774. doi: 10.1093/infdis/151.5.772. [DOI] [PubMed] [Google Scholar]
- Landry M. L., Zibello T. A., Hsiung G. D. Comparison of in situ hybridization and immunologic staining with cytopathology for detection and identification of herpes simplex virus infection in cultured cells. J Clin Microbiol. 1986 Dec;24(6):968–971. doi: 10.1128/jcm.24.6.968-971.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lurain N. S., Thompson K. D., Farrand S. K. Rapid detection of cytomegalovirus in clinical specimens by using biotinylated DNA probes and analysis of cross-reactivity with herpes simplex virus. J Clin Microbiol. 1986 Nov;24(5):724–730. doi: 10.1128/jcm.24.5.724-730.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myerson D., Hackman R. C., Meyers J. D. Diagnosis of cytomegaloviral pneumonia by in situ hybridization. J Infect Dis. 1984 Aug;150(2):272–277. doi: 10.1093/infdis/150.2.272. [DOI] [PubMed] [Google Scholar]
- Przepiorka D., Myerson D. A single-step silver enhancement method permitting rapid diagnosis of cytomegalovirus infection in formalin-fixed, paraffin-embedded tissue sections by in situ hybridization and immunoperoxidase detection. J Histochem Cytochem. 1986 Dec;34(12):1731–1734. doi: 10.1177/34.12.3023477. [DOI] [PubMed] [Google Scholar]
- Richman D. D., Cleveland P. H., Redfield D. C., Oxman M. N., Wahl G. M. Rapid viral diagnosis. J Infect Dis. 1984 Mar;149(3):298–310. doi: 10.1093/infdis/149.3.298. [DOI] [PubMed] [Google Scholar]
- Seto E., Yen T. S. Detection of cytomegalovirus infection by means of DNA isolated from paraffin-embedded tissues and dot hybridization. Am J Pathol. 1987 Jun;127(3):409–413. [PMC free article] [PubMed] [Google Scholar]
- Shuster E. A., Beneke J. S., Tegtmeier G. E., Pearson G. R., Gleaves C. A., Wold A. D., Smith T. F. Monoclonal antibody for rapid laboratory detection of cytomegalovirus infections: characterization and diagnostic application. Mayo Clin Proc. 1985 Sep;60(9):577–585. doi: 10.1016/s0025-6196(12)60979-3. [DOI] [PubMed] [Google Scholar]
- Swenson P. D., Kaplan M. H. Rapid detection of cytomegalovirus in cell culture by indirect immunoperoxidase staining with monoclonal antibody to an early nuclear antigen. J Clin Microbiol. 1985 May;21(5):669–673. doi: 10.1128/jcm.21.5.669-673.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unger E. R., Budgeon L. R., Myerson D., Brigati D. J. Viral diagnosis by in situ hybridization. Description of a rapid simplified colorimetric method. Am J Surg Pathol. 1986 Jan;10(1):1–8. doi: 10.1097/00000478-198601000-00001. [DOI] [PubMed] [Google Scholar]
