Fig. 3. Time courses of increases in fluorescence intensity upon addition of egg PC SUV to apoA-I V53C-pyrene (trace a) and F229C-pyrene (trace b).
SUV was added to apoA-I variants at final concentrations of 25 μg/ml protein and 1.0 mg/ml PC. Pyrene fluorescence was monitored at 385 nm with an excitation of 342 nm.