Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1988 Oct;26(10):1962–1966. doi: 10.1128/jcm.26.10.1962-1966.1988

Coagglutination of Vibrio cholerae, Vibrio mimicus, and Vibrio vulnificus with anti-flagellar monoclonal antibody.

J G Simonson 1, R J Siebeling 1
PMCID: PMC266798  PMID: 3182988

Abstract

Monoclonal antibodies (MAbs) with serological activity for purified flagellar (H) core protein prepared from Vibrio cholerae were identified by enzyme-linked immunosorbent assay. Four of these MAbs reacted with the flagella of V. cholerae and V. mimicus exclusively, while eight MAbs reacted with at least 1 of 30 heterologous Vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. It appears that V. cholerae and V. mimicus express similar, if not identical, H determinants unique to these two Vibrio species. Staphylococcus aureus cells or latex beads armed with the four species-specific MAbs coagglutinated each of 47 isolates identified bacteriologically as V. cholerae or V. mimicus from among 103 Vibrio isolates tested. One coagglutination reagent armed with anti-V. vulnificus H MAb exhibited species specificity in that only V. vulnificus cells were coagglutinated from among the 31 Vibrio species examined. This reagent coagglutinated 20 isolates identified bacteriologically as V. vulnificus in a serological survey. MAb coagglutination reagents offer a rapid, specific, and economical alternative to the classical bacteriological approach to identify the human pathogens V. cholerae, V. mimicus, and V. vulnificus.

Full text

PDF
1962

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhattacharyya F. K., Mukerjee S. Serological analysis of the flagellar or H agglutining antigens of cholera and NAG vibrios. Ann Microbiol (Paris) 1974 Feb-Mar;125A(2):167–181. [PubMed] [Google Scholar]
  2. Bhattacharyya F. K. Vibrio cholerae flagellar antigens: a serodiagnostic test, functional implications of H-reactivity and taxonomic importance of cross-reactions within the Vibrio genus. Med Microbiol Immunol. 1975 Dec 30;162(1):29–41. doi: 10.1007/BF02123575. [DOI] [PubMed] [Google Scholar]
  3. Brenner D. J., Davis B. R., Kudoh Y., Ohashi M., Sakazaki R., Shimada T., Smith H. L., Jr Serological comparison of two collections of Vibrio cholerae non O1. J Clin Microbiol. 1982 Aug;16(2):319–323. doi: 10.1128/jcm.16.2.319-323.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Douillard J. Y., Hoffman T. Enzyme-linked immunosorbent assay for screening monoclonal antibody production using enzyme-labeled second antibody. Methods Enzymol. 1983;92:168–174. doi: 10.1016/0076-6879(83)92016-5. [DOI] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. Miwatani T., Shinoda S. Flagellar antigen of Vibrio alginolyticus. Biken J. 1971 Dec;14(4):389–394. [PubMed] [Google Scholar]
  7. Pastoris M. C., Bhattacharyya F. K., Sil J. Evaluation of the phenol-induced flagellar agglutination test for the identification of the cholera group of vibrios. J Med Microbiol. 1980 May;13(2):362–367. doi: 10.1099/00222615-13-2-363. [DOI] [PubMed] [Google Scholar]
  8. Rahman M., Sack D. A., Mahmood S., Hossain A. Rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures. J Clin Microbiol. 1987 Nov;25(11):2204–2206. doi: 10.1128/jcm.25.11.2204-2206.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Richardson K., Parker C. D. Identification and occurrence of Vibrio cholerae flagellar core proteins in isolated outer membrane. Infect Immun. 1985 Mar;47(3):674–679. doi: 10.1128/iai.47.3.674-679.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sakazaki R., Iwanami S., Tamura K. Studies on the enteropathogenic, facultatively halophilic bacterium, Vibrio parahaemolyticus. II. Serological characteristics. Jpn J Med Sci Biol. 1968 Oct;21(5):313–324. doi: 10.7883/yoken1952.21.313. [DOI] [PubMed] [Google Scholar]
  11. Shinoda S., Miwatani T., Fujino T. A common antigenic substance of Vibrio parahaemolyticus. II. Some physicochemical properties. Biken J. 1971 Mar;14(1):75–76. [PubMed] [Google Scholar]
  12. Shinoda S., Nakahara N., Ninomiya Y., Itoh K., Kane H. Serological method for identification of Vibrio parahaemolyticus from marine samples. Appl Environ Microbiol. 1983 Jan;45(1):148–152. doi: 10.1128/aem.45.1.148-152.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sil J., Bhattacharyya F. K. A rapid test for the identification of all serotypes of Vibrio cholerae (including "non-agglutinating" vibrios). J Med Microbiol. 1979 Feb;12(1):63–70. doi: 10.1099/00222615-12-1-63. [DOI] [PubMed] [Google Scholar]
  14. Simonson J., Siebeling R. J. Rapid serological identification of Vibrio vulnificus by anti-H coagglutination. Appl Environ Microbiol. 1986 Dec;52(6):1299–1304. doi: 10.1128/aem.52.6.1299-1304.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith H. L., Jr Serotyping of non-cholera vibrios. J Clin Microbiol. 1979 Jul;10(1):85–90. doi: 10.1128/jcm.10.1.85-90.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tassin M. G., Siebeling R. J., Roberts N. C., Larson A. D. Presumptive identification of Vibrio species with H antiserum. J Clin Microbiol. 1983 Aug;18(2):400–407. doi: 10.1128/jcm.18.2.400-407.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yang G. C., Schrank G. D., Freeman B. A. Purification of flagellar cores of Vibrio cholerae. J Bacteriol. 1977 Feb;129(2):1121–1128. doi: 10.1128/jb.129.2.1121-1128.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES