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. 1988 Oct;26(10):2087–2091. doi: 10.1128/jcm.26.10.2087-2091.1988

Immunoblot analysis of serologic response to outer membrane proteins of Escherichia coli in elderly individuals with urinary tract infections.

L E Nicolle 1, E Ujack 1, J Brunka 1, L E Bryan 1
PMCID: PMC266821  PMID: 3053763

Abstract

We used immunoblotting to examine the serologic antibody responses to outer membrane proteins (OMP) of Escherichia coli in both symptomatic and asymptomatic elderly subjects with urinary tract infections. Controls with no present or past urinary tract infections showed variable weak immunoglobulin G (IgG) antibodies to OMP of infecting strains. Elderly individuals with asymptomatic infections demonstrated antibody to both lipopolysaccharide (LPS) and OMP of their infecting strain, with consistent cross-reactivity to OMP of other infecting strains. Young females with acute pyelonephritis showed an IgG response to LPS and OMP with cross-reactivity to OMP of other strains. Elderly individuals with symptomatic invasive infections had strong reactions to both LPS and OMP in specimens collected during the acute phase, generally with an increase in intensity in specimens from convalescent patients. They also demonstrated extensive cross-reactivity to LPS and OMP from all other infecting strains. IgM antibody was not observed in any patients. These data confirm other reports of low levels of antibodies to OMP of E. coli in normal populations. Asymptomatic bacteriuria in this population is associated with antibody responses to the LPS and OMP of the infecting strain. Elderly individuals with invasive infections had initial reactions to the infecting strain with an apparent increase in intensity during convalescence. Antibodies to the major OMP appear to be broadly cross-reactive.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boscia J. A., Kobasa W. D., Knight R. A., Abrutyn E., Levison M. E., Kaye D. Epidemiology of bacteriuria in an elderly ambulatory population. Am J Med. 1986 Feb;80(2):208–214. doi: 10.1016/0002-9343(86)90011-2. [DOI] [PubMed] [Google Scholar]
  2. Esposito A. L., Gleckman R. A., Cram S., Crowley M., McCabe F., Drapkin M. S. Community-acquired bacteremia in the elderly: analysis of one hundred consecutive episodes. J Am Geriatr Soc. 1980 Jul;28(7):315–319. doi: 10.1111/j.1532-5415.1980.tb00622.x. [DOI] [PubMed] [Google Scholar]
  3. Hancock R. E., Nikaido H. Outer membranes of gram-negative bacteria. XIX. Isolation from Pseudomonas aeruginosa PAO1 and use in reconstitution and definition of the permeability barrier. J Bacteriol. 1978 Oct;136(1):381–390. doi: 10.1128/jb.136.1.381-390.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hofstra H., Dankert J. Major outer membrane proteins: common antigens in enterobacteriaceae species. J Gen Microbiol. 1980 Jul;119(1):123–131. doi: 10.1099/00221287-119-1-123. [DOI] [PubMed] [Google Scholar]
  5. Holmgren J., Smith J. W. Immunological aspects of urinary tract infections. Prog Allergy. 1975;18:289–352. [PubMed] [Google Scholar]
  6. Kaye D. Urinary tract infections in the elderly. Bull N Y Acad Med. 1980 Mar;56(2):209–220. [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Nicolle L. E., Bjornson J., Harding G. K., MacDonell J. A. Bacteriuria in elderly institutionalized men. N Engl J Med. 1983 Dec 8;309(23):1420–1425. doi: 10.1056/NEJM198312083092304. [DOI] [PubMed] [Google Scholar]
  10. Nicolle L. E., Mayhew W. J., Bryan L. Prospective randomized comparison of therapy and no therapy for asymptomatic bacteriuria in institutionalized elderly women. Am J Med. 1987 Jul;83(1):27–33. doi: 10.1016/0002-9343(87)90493-1. [DOI] [PubMed] [Google Scholar]
  11. Nicolle L. E., McIntyre M., Zacharias H., MacDonell J. A. Twelve-month surveillance of infections in institutionalized elderly men. J Am Geriatr Soc. 1984 Jul;32(7):513–519. doi: 10.1111/j.1532-5415.1984.tb02236.x. [DOI] [PubMed] [Google Scholar]
  12. Overbeeke N., Lugtenberg B. Major outer membrane proteins of Escherichia coli strains of human origin. J Gen Microbiol. 1980 Dec;121(2):373–380. doi: 10.1099/00221287-121-2-373. [DOI] [PubMed] [Google Scholar]
  13. Shand G. H., Anwar H., Kadurugamuwa J., Brown M. R., Silverman S. H., Melling J. In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infect Immun. 1985 Apr;48(1):35–39. doi: 10.1128/iai.48.1.35-39.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weksler M. E. The senescence of the immune system. Hosp Pract (Off Ed) 1981 Oct;16(10):53–64. doi: 10.1080/21548331.1981.11946847. [DOI] [PubMed] [Google Scholar]

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