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. 1988 Oct;26(10):2195–2199. doi: 10.1128/jcm.26.10.2195-2199.1988

Modified spin-amplified adsorption procedure with conventional tissue culture tubes for rapid detection and increased recovery of herpes simplex virus from clinical specimens.

P E Oefinger 1, S H Loo 1, R M Gander 1
PMCID: PMC266847  PMID: 2846642

Abstract

Conventional culture tubes were used in a modification of the spin-amplified adsorption procedure for recovery of herpes simplex virus (HSV) from clinical specimens. The sensitivity of isolation of HSV from 864 specimens adsorbed by the spin-amplified method was 100% (127 of 127), compared with 88.2% (112 of 127) for stationary-phase-adsorbed specimens. Cytopathic effect developed more rapidly in 32.1% (36 of 112) of isolates adsorbed by spin amplification than in those adsorbed by stationary means. In a separate quantitative study, cultures of HSV type 1 adsorbed by spin amplification yielded higher antigen levels and greater cytopathic effect than stationary-phase-adsorbed cultures. Cells grown in conventional tissue culture tubes may be used in a spin-amplified adsorption for rapid detection and increased sensitivity of HSV isolation.

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Selected References

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  1. GORDON F. B., QUAN A. L., TRIMMER R. W. Morphologic observations on trachoma virus in cell cultures. Science. 1960 Mar 11;131(3402):733–734. doi: 10.1126/science.131.3402.733. [DOI] [PubMed] [Google Scholar]
  2. Gleaves C. A., Smith T. F., Shuster E. A., Pearson G. R. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J Clin Microbiol. 1984 Jun;19(6):917–919. doi: 10.1128/jcm.19.6.917-919.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gleaves C. A., Wilson D. J., Wold A. D., Smith T. F. Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation. J Clin Microbiol. 1985 Jan;21(1):29–32. doi: 10.1128/jcm.21.1.29-32.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hudson J. B., Misra V., Mosmann T. R. Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity. Virology. 1976 Jul 1;72(1):235–243. doi: 10.1016/0042-6822(76)90326-3. [DOI] [PubMed] [Google Scholar]
  5. Michalski F. J., Shaikh M., Sahraie F., Desai S., Verano L., Vallabhaneni J. Enzyme-linked immunosorbent assay spin amplification technique for herpes simplex virus antigen detection. J Clin Microbiol. 1986 Aug;24(2):310–311. doi: 10.1128/jcm.24.2.310-311.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morgan M. A., Smith T. F. Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen. J Clin Microbiol. 1984 Jun;19(6):730–732. doi: 10.1128/jcm.19.6.730-732.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Osborn J. E., Walker D. L. Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation. J Virol. 1968 Sep;2(9):853–858. doi: 10.1128/jvi.2.9.853-858.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pruneda R. C., Almanza I. Centrifugation-shell vial technique for rapid detection of herpes simplex virus cytopathic effect in Vero cells. J Clin Microbiol. 1987 Feb;25(2):423–424. doi: 10.1128/jcm.25.2.423-424.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Salmon V. C., Turner R. B., Speranza M. J., Overall J. C., Jr Rapid detection of herpes simplex virus in clinical specimens by centrifugation and immunoperoxidase staining. J Clin Microbiol. 1986 Apr;23(4):683–686. doi: 10.1128/jcm.23.4.683-686.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Shuster E. A., Beneke J. S., Tegtmeier G. E., Pearson G. R., Gleaves C. A., Wold A. D., Smith T. F. Monoclonal antibody for rapid laboratory detection of cytomegalovirus infections: characterization and diagnostic application. Mayo Clin Proc. 1985 Sep;60(9):577–585. doi: 10.1016/s0025-6196(12)60979-3. [DOI] [PubMed] [Google Scholar]
  11. Smith E. M., Gerba C. P. Development of a method for detection of human rotavirus in water and sewage. Appl Environ Microbiol. 1982 Jun;43(6):1440–1450. doi: 10.1128/aem.43.6.1440-1450.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Swenson P. D., Kaplan M. H. Comparison of two rapid culture methods for detection of cytomegalovirus in clinical specimens. J Clin Microbiol. 1987 Dec;25(12):2445–2446. doi: 10.1128/jcm.25.12.2445-2446.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WEISS E., DRESSLER H. R. Centrifugation and Rickettsiae and viruses onto cells and its effect on infection. Proc Soc Exp Biol Med. 1960 Apr;103:691–695. doi: 10.3181/00379727-103-25637. [DOI] [PubMed] [Google Scholar]

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