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. 1988 Nov;26(11):2297–2299. doi: 10.1128/jcm.26.11.2297-2299.1988

Value of semiquantitative cultures of blood drawn through catheter hubs for estimating the risk of catheter tip colonization in cancer patients.

A Andremont 1, R Paulet 1, G Nitenberg 1, C Hill 1
PMCID: PMC266880  PMID: 3069861

Abstract

Colonization of central catheter tips has been associated with catheter-related infections. This colonization is defined as the presence of over 15 CFU in a semiquantitative catheter tip culture performed after catheter removal. Using a simple pour-plate technique, we determined the microbial concentrations in samples of blood collected via the hubs of 205 central catheters while they were in position in 179 cancer patients. All catheters were removed within three days of blood collection via the hub and cultured semiquantitatively. We then compared the results for the hub blood cultures and catheter tip cultures. Cultures from 18% of the hub samples and 29% of the tips were positive. When a cutoff limit of 1,000 CFU/ml was used for the blood cultures, the sensitivity was 20% and the specificity was 99% for estimating catheter tip colonization. These values did not seem to be related to the underlying disease, the site of catheter insertion, or the antibiotic treatment administered at catheter removal. We conclude that, if positive, cultures of blood sampled via the catheter hub can be useful in assessing the risk of catheter colonization in cancer patients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong C. W., Mayhall C. G., Miller K. B., Newsome H. H., Jr, Sugerman H. J., Dalton H. P., Hall G. O., Gennings C. Prospective study of catheter replacement and other risk factors for infection of hyperalimentation catheters. J Infect Dis. 1986 Nov;154(5):808–816. doi: 10.1093/infdis/154.5.808. [DOI] [PubMed] [Google Scholar]
  2. Ascuitto R. J., Gerber M. A., Cates K. L., Tilton R. C. Buffy coat smears of blood drawn through central venous catheters as an aid to rapid diagnosis of systemic fungal infections. J Pediatr. 1985 Mar;106(3):445–447. doi: 10.1016/s0022-3476(85)80675-2. [DOI] [PubMed] [Google Scholar]
  3. Bjornson H. S., Colley R., Bower R. H., Duty V. P., Schwartz-Fulton J. T., Fischer J. E. Association between microorganism growth at the catheter insertion site and colonization of the catheter in patients receiving total parenteral nutrition. Surgery. 1982 Oct;92(4):720–727. [PubMed] [Google Scholar]
  4. Brun Buisson C., Rauss A., Legrand P. Semiquantitative culture of catheter tips. J Clin Microbiol. 1987 Jul;25(7):1343–1344. doi: 10.1128/jcm.25.7.1343-1344.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brun-Buisson C., Abrouk F., Legrand P., Huet Y., Larabi S., Rapin M. Diagnosis of central venous catheter-related sepsis. Critical level of quantitative tip cultures. Arch Intern Med. 1987 May;147(5):873–877. [PubMed] [Google Scholar]
  6. Christensen G. D., Simpson W. A., Bisno A. L., Beachey E. H. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun. 1982 Jul;37(1):318–326. doi: 10.1128/iai.37.1.318-326.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cleri D. J., Corrado M. L., Seligman S. J. Quantitative culture of intravenous catheters and other intravascular inserts. J Infect Dis. 1980 Jun;141(6):781–786. doi: 10.1093/infdis/141.6.781. [DOI] [PubMed] [Google Scholar]
  8. Collignon P. J., Soni N., Pearson I. Y., Woods W. P., Munro R., Sorrell T. C. Is semiquantitative culture of central vein catheter tips useful in the diagnosis of catheter-associated bacteremia? J Clin Microbiol. 1986 Oct;24(4):532–535. doi: 10.1128/jcm.24.4.532-535.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cooper G. L., Hopkins C. C. Rapid diagnosis of intravascular catheter-associated infection by direct Gram staining of catheter segments. N Engl J Med. 1985 May 2;312(18):1142–1147. doi: 10.1056/NEJM198505023121802. [DOI] [PubMed] [Google Scholar]
  10. Fagan T. J. Letter: Nomogram for Bayes theorem. N Engl J Med. 1975 Jul 31;293(5):257–257. doi: 10.1056/NEJM197507312930513. [DOI] [PubMed] [Google Scholar]
  11. Johnson P. R., Decker M. D., Edwards K. M., Schaffner W., Wright P. F. Frequency of broviac catheter infections in pediatric oncology patients. J Infect Dis. 1986 Oct;154(4):570–578. doi: 10.1093/infdis/154.4.570. [DOI] [PubMed] [Google Scholar]
  12. Maki D. G., Weise C. E., Sarafin H. W. A semiquantitative culture method for identifying intravenous-catheter-related infection. N Engl J Med. 1977 Jun 9;296(23):1305–1309. doi: 10.1056/NEJM197706092962301. [DOI] [PubMed] [Google Scholar]
  13. Moyer M. A., Edwards L. D., Farley L. Comparative culture methods on 101 intravenous catheters. Routine, semiquantitative, and blood cultures. Arch Intern Med. 1983 Jan;143(1):66–69. [PubMed] [Google Scholar]
  14. Raucher H. S., Hyatt A. C., Barzilai A., Harris M. B., Weiner M. A., LeLeiko N. S., Hodes D. S. Quantitative blood cultures in the evaluation of septicemia in children with Broviac catheters. J Pediatr. 1984 Jan;104(1):29–33. doi: 10.1016/s0022-3476(84)80584-3. [DOI] [PubMed] [Google Scholar]
  15. Schuman E. S., Winters V., Gross G. F., Hayes J. F. Management of Hickman catheter sepsis. Am J Surg. 1985 May;149(5):627–628. doi: 10.1016/s0002-9610(85)80142-2. [DOI] [PubMed] [Google Scholar]
  16. Snydman D. R., Murray S. A., Kornfeld S. J., Majka J. A., Ellis C. A. Total parenteral nutrition-related infections. Prospective epidemiologic study using semiquantitative methods. Am J Med. 1982 Nov;73(5):695–699. doi: 10.1016/0002-9343(82)90412-0. [DOI] [PubMed] [Google Scholar]
  17. Whimbey E., Wong B., Kiehn T. E., Armstrong D. Clinical correlations of serial quantitative blood cultures determined by lysis-centrifugation in patients with persistent septicemia. J Clin Microbiol. 1984 Jun;19(6):766–771. doi: 10.1128/jcm.19.6.766-771.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wing E. J., Norden C. W., Shadduck R. K., Winkelstein A. Use of quantitative bacteriologic techniques to diagnose catheter-related sepsis. Arch Intern Med. 1979 Apr;139(4):482–483. [PubMed] [Google Scholar]

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