Abstract
Leptospira interrogans serovar lai was identified in China in 1966 as a new serovar of the icterohaemorrhagiae serogroup by cross-absorption tests. In this study, we established three hybridoma cell lines producing monoclonal antibodies (MAbs) of the immunoglobulin G3 subclass (LW1, LW2, and LW3) and of the immunoglobulin M class (LW4a) against serovar lai strain 017 by the cell fusion technique. Immunological reactivities of the MAbs were determined by the microscopic agglutination test, enzyme-linked immunosorbent assay (ELISA), and indirect immunofluorescence assay. MAbs LW1 and LW3 agglutinated cells of serovars lai, birkini, and gem of serogroup icterohaemorrhagiae. LW2 agglutinated various serovars of serogroup icterohaemorrhagiae, except for serovar tonkini. LW4a reacted positively with various Leptospira species, including a new species, Leptospira parva, in the ELISA and indirect immunofluorescence assay. However, LW4a did not react with Leptonema illini 3055. The results of an inhibition ELISA with heated outer envelope (OE) or periodate-oxidized OE suggested that these MAbs recognize a carbohydrate moiety of the OE as the antigenic determinant.
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- Adler B., Faine S. Species- and genus-specific antigens in Leptospira, revealed by monoclonal antibodies and enzyme immunoassay. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Sep;255(2-3):317–322. [PubMed] [Google Scholar]
- Adler B., Murphy A. M., Locarnini S. A., Faine S. Detection of specific anti-leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay. J Clin Microbiol. 1980 May;11(5):452–457. doi: 10.1128/jcm.11.5.452-457.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Auran N. E., Johnson R. C., Ritzi D. M. Isolation of the outer sheath of Leptospira and its immunogenic properties in hamsters. Infect Immun. 1972 Jun;5(6):968–975. doi: 10.1128/iai.5.6.968-975.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kobayashi Y., Tamai T., Oyama T., Hasegawa H., Sada E., Kusaba T., Hamaji M. Characterization of monoclonal antibodies against etiological agents of Weil's disease. Microbiol Immunol. 1984;28(3):359–370. doi: 10.1111/j.1348-0421.1984.tb00687.x. [DOI] [PubMed] [Google Scholar]
- Kobayashi Y., Tamai T., Sada E. Serological analysis of serogroup icterohaemorrhagiae using monoclonal antibodies. Microbiol Immunol. 1985;29(12):1229–1235. doi: 10.1111/j.1348-0421.1985.tb00912.x. [DOI] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Lukehart S. A., Tam M. R., Hom J., Baker-Zander S. A., Holmes K. K., Nowinski R. C. Characterization of monoclonal antibodies to Treponema pallidum. J Immunol. 1985 Jan;134(1):585–592. [PubMed] [Google Scholar]
- OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. Prog Allergy. 1958;5:1–78. [PubMed] [Google Scholar]
- Ono E., Naiki M., Yanagawa R. Production and characterization of monoclonal antibodies to lipopolysaccharide antigen of Leptospira interrogans serovar kremastos and canicola. Zentralbl Bakteriol Mikrobiol Hyg A. 1982 Jul;252(3):414–424. [PubMed] [Google Scholar]
- Sakamoto N., Ono E., Kida H., Yanagawa R. Characterization of a partially purified leptospiral genus-specific protein antigen. Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Jul;259(4):507–519. doi: 10.1016/s0176-6724(85)80083-3. [DOI] [PubMed] [Google Scholar]
- Sakamoto N., Ono E., Kida H., Yanagawa R. Production of monoclonal antibodies against leptospiral genus-specific protein antigen and localization of the antigen by immunoelectron microscopy. Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Jul;259(4):557–563. doi: 10.1016/s0176-6724(85)80088-2. [DOI] [PubMed] [Google Scholar]